Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,925 T→A 41.6% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,9250TA41.6% 73.5 / 143.7 127intergenic (‑1008/‑160)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (35/39);  new base A (35/18);  total (70/57)
Fisher's exact test for biased strand distribution p-value = 4.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 130 total aligned reads displayed.

GTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATAC  >  CP000730/1929789‑1930060
                                                                                                                                             |                                                                                                                                              
gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                                                                            <  1:652921/140‑3 (MQ=255)
   tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                                                                                                         >  2:424596/9‑135 (MQ=255)
     ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                                                                       >  1:378478/7‑140 (MQ=255)
      tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                      >  2:262819/6‑140 (MQ=255)
      tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                      >  2:223806/6‑140 (MQ=255)
      tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATGACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                      <  2:226637/135‑1 (MQ=255)
       cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGATGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                     >  1:297137/5‑140 (MQ=255)
       cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                     >  1:262654/5‑140 (MQ=255)
       cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                     >  2:250494/5‑140 (MQ=255)
         ccGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTg                                                                                                                                    >  2:156940/1‑140 (MQ=255)
         cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAAAGGAGGTGCATTACTTTGCTAATCATTTTCGGTAACAACATAGGACCAGTCATCAGTGGCTGTGCTGTTTCGTATTATACTTATTgg                                                                                                                                   >  1:337951/3‑140 (MQ=255)
          agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                                  <  2:36775/139‑1 (MQ=255)
           gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                                                                                  <  1:437332/140‑1 (MQ=255)
           gatgatATAATAGTAAAGTCGCCTATCTCTCAGGCGTCAATTTATACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                                                                                  <  1:110705/140‑1 (MQ=255)
              ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTACTATACTTATTGGCTTAg                                                                                                                              <  2:335683/138‑1 (MQ=255)
               ttataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                             <  2:615958/139‑1 (MQ=255)
               gttaaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                             <  2:332591/136‑1 (MQ=255)
               atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                              <  2:495638/140‑1 (MQ=255)
                tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                            >  2:558378/1‑140 (MQ=255)
                tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAATTTGCTAATCATTTTCGTTCACATCATAGCACCATTCATCAGTGGCTTTTCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                            >  2:157312/1‑140 (MQ=255)
                 ttaataGTAGAGCCGCTGATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                                                                                            >  2:146383/2‑140 (MQ=255)
                 cgatctatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTGACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                           >  2:6591/8‑139 (MQ=255)
                 ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCCGTGGCTGTGCTGTTGCGTATTATACTTCTTGGcggagaat                                                                                                                           >  2:135965/1‑133 (MQ=255)
                  taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATAATAGCACCAGTTATCAGTGGCTGTGCCAATGCATTTTTTTCTTATTGGCTAAGTAg                                                                                                                           <  1:28062/140‑1 (MQ=255)
                   aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                                                                         >  1:667840/1‑140 (MQ=255)
                    ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCTTTAGTTCTTAAAGCAATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                        <  1:154182/140‑1 (MQ=255)
                    ataCTAGAGTAGCCTATCTCTCAGGCAAAAATT‑‑GAAGAAGAGAGGAGGTGAATTAATTTGAAAAGCATTTTCAGTTGCATCAGATCACCAGTCATCAGTGGCTGTGGTGTTGCGTATGATACTTATTGGCTTAGTAAACg                                                                                                                        <  2:657606/140‑1 (MQ=255)
                      agtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTACTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                                                                       <  1:116665/140‑1 (MQ=255)
                       atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGACGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                                                                     >  2:433154/2‑140 (MQ=255)
                           agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCATTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                                                                                  >  2:371126/1‑140 (MQ=255)
                           agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                 >  1:644172/1‑136 (MQ=255)
                           agTCGCCTACCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTTATCAGTGGCTGTGTCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                                                                                  <  1:173036/140‑1 (MQ=255)
                            gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                >  1:438656/1‑135 (MQ=255)
                            gTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGATCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATCCTTATTGGCTTAGTAAACGCaaaaaat                                                                                                                >  2:188322/1‑135 (MQ=255)
                            gTCGCCTATATCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                >  2:251746/1‑135 (MQ=255)
                             tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTTCTCAATTGCCGCCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                               <  1:484475/140‑7 (MQ=255)
                               gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑aa‑‑aa                                                                                                          <  2:177297/140‑1 (MQ=255)
                                ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AAt                                                                                                         >  1:264990/1‑140 (MQ=255)
                                ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                            >  2:324772/1‑131 (MQ=255)
                                ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                            >  1:30862/1‑131 (MQ=255)
                                ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                            >  1:53523/1‑131 (MQ=255)
                                 cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                           >  2:100951/1‑130 (MQ=255)
                                  tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGATGTTGCGTATAATACTTATTGGCTTGGTAAACGCaacaaataattaa                                                                                                          >  1:451416/1‑129 (MQ=255)
                                  tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                          >  1:418785/1‑129 (MQ=255)
                                   aTCTCTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                         <  2:214678/140‑13 (MQ=255)
                                   aTCTCTCAGTCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACCTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                         <  2:532574/140‑13 (MQ=255)
                                   aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                         >  1:314945/1‑128 (MQ=255)
                                     ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                                                                                       <  2:660952/140‑15 (MQ=255)
                                     ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                                                                                       >  2:607649/1‑126 (MQ=255)
                                          aGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACata                                                                                              >  2:53056/1‑140 (MQ=255)
                                               tccaTTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGcc                                                                                        <  2:654316/137‑1 (MQ=255)
                                                 aaTTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGc                                                                                     >  2:298503/1‑140 (MQ=255)
                                                  aattgacgcctgagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCa                                                                                    <  1:45457/129‑1 (MQ=255)
                                                  aTTTAGGCGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCa                                                                                    <  2:117695/140‑1 (MQ=255)
                                                    ttAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTTACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCAcc                                                                                  >  2:355254/1‑140 (MQ=255)
                                                          gCAGATACGAGTTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATCGGCTAAGTCGCCGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATaaa                                                                            <  1:657444/140‑1 (MQ=255)
                                                            agagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATaaaaa                                                                          <  1:546191/140‑1 (MQ=255)
                                                             gagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGAACCAATAAAAAt                                                                         >  1:373733/1‑140 (MQ=255)
                                                              agagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATc                                                                        <  1:581282/140‑1 (MQ=255)
                                                              agagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATc                                                                        >  1:442154/1‑140 (MQ=255)
                                                                gggaggGGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCCAAAAAAATcac                                                                      >  2:541456/2‑138 (MQ=255)
                                                                 ggaggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATcccc                                                                     <  1:420496/140‑1 (MQ=255)
                                                                  gaggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                                                                    <  2:375683/140‑1 (MQ=255)
                                                                    ggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCCTTGCGTTTTTTTCTTATTGGCTAAGTAGAGGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCACTAAAAATCCCCCCa                                                                  >  2:293383/1‑140 (MQ=255)
                                                                     gtgtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc                                                                 >  1:556422/1‑140 (MQ=255)
                                                                      tgtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACt                                                                <  1:208517/140‑1 (MQ=255)
                                                                       gtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTa                                                               >  1:634105/1‑140 (MQ=255)
                                                                       gtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGAAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCACCTCACTa                                                               >  1:433186/1‑140 (MQ=255)
                                                                            aaGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGc                                                           <  1:359776/140‑1 (MQ=255)
                                                                             aGGTGATGCTTATTTTCTTTCACATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑CCCCGGTCACAGATAGCCGCACCAATAAAAATCCCCTCACTACCGCa                                                          <  2:607591/140‑1 (MQ=255)
                                                                                 gTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa                                                        >  2:255216/1‑140 (MQ=255)
                                                                                 gTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTACGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa                                                        >  1:17989/1‑140 (MQ=255)
                                                                                   tgttGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTatttgcggt                                                       <  1:601680/137‑9 (MQ=255)
                                                                                    taTGCTTATTTTTGTTCACATCATAGCACCAGTCATCAGTGGCTTTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATa                                                      <  1:301929/139‑1 (MQ=255)
                                                                                         cTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGa                                                  >  1:60442/1‑140 (MQ=255)
                                                                                          ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGCCATATAGCCGCACCAATACACAACCCCTCACTACCGCAAGTaacgcg                                                 >  2:552250/1‑135 (MQ=255)
                                                                                          ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATCGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATTAAAATCCCCTCACTACCACAAagagagag                                                 >  2:424809/1‑140 (MQ=255)
                                                                                           tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                <  2:58478/140‑1 (MQ=255)
                                                                                           tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGGTTTGTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                >  1:457144/1‑140 (MQ=255)
                                                                                           tATTTCCGTTCCTATTATAGCCCCAGTCATCAGTGGCTGTGCCATTTCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                <  2:355065/140‑1 (MQ=255)
                                                                                             ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                              <  1:130121/140‑1 (MQ=255)
                                                                                             ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                              <  1:290266/140‑1 (MQ=255)
                                                                                                         tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCCAAAAGTGAGGGGATTGGTGTATaa                                  <  2:643649/140‑1 (MQ=255)
                                                                                                         tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGAATaa                                  >  2:665917/1‑140 (MQ=255)
                                                                                                          catAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAg                                 <  1:146383/140‑1 (MQ=255)
                                                                                                            tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGAAAAAATCCCCACACTACTGCAATAGTGTGGGGATTGgtgcat                                    <  1:102347/140‑4 (MQ=255)
                                                                                                               cACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCAACAATAAAAATCCCCTCACTACCGCAAATAATGAGGGGATTGGTGTATAAGTAAAt                            >  1:196179/1‑140 (MQ=255)
                                                                                                                aCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATa                           >  1:185855/1‑140 (MQ=255)
                                                                                                                    gTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTa                       >  1:562388/1‑140 (MQ=255)
                                                                                                                      catcaGTGGCTGCGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGATATATAGCCGCACCAATAAAAATCCCCTCACTAGCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt                     <  1:53056/140‑1 (MQ=255)
                                                                                                                       atcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                    <  1:240119/140‑1 (MQ=255)
                                                                                                                       atcaGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTATCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                    <  1:343325/140‑1 (MQ=255)
                                                                                                                         caGTGGCTGTGCCATTGCGTTTTTTTTTTGTTGGGTGGGAAGACGCAATAC‑‑AA‑‑AAGAGGAGACGTATAGCCGCACCAATGAAAATCCCCTCACTACCGCAAATAGTAGGGGGATTGGTGTATAAGGAAATACTTTTTTTc                  >  2:341920/1‑140 (MQ=255)
                                                                                                                         caGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑CA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTc                  >  1:221683/1‑140 (MQ=255)
                                                                                                                                tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCt           >  2:374244/1‑140 (MQ=255)
                                                                                                                                  tgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAACACTTATTTTCGTTGTCTTa         <  1:371126/140‑1 (MQ=255)
                                                                                                                                   gCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTaa        <  1:637807/140‑1 (MQ=255)
                                                                                                                                     cATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGAGTATAAGTAAATACTTATTTTCGTTGTCTTAAtt      >  1:611717/1‑140 (MQ=255)
                                                                                                                                      aTTGCGTTTTTTTCGTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTTCCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATta     <  1:180334/140‑1 (MQ=255)
                                                                                                                                         gCGTTTTTTTCTCTTTGGCTAAGTAGACTCAATAC‑‑AA‑‑AATAGGGCACATATAGCCGCACCAATAAAAATCCCCTCACTTCCGTAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTTTCTTAATTATAc  <  2:43113/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                              
GTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATAC  >  CP000730/1929789‑1930060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: