Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,941 A→T 35.8% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,9410AT35.8% 82.9 / 110.5 110intergenic (‑1024/‑144)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/37);  new base T (26/13);  total (60/50)
Fisher's exact test for biased strand distribution p-value = 7.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-01

Only 100 of 110 total aligned reads displayed.

ATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATT  >  CP000730/1929804‑1930068
                                                                                                                                            |                                                                                                                                      
ttataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     <  2:615958/139‑1 (MQ=255)
gttaaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     <  2:332591/136‑1 (MQ=255)
gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     >  1:144792/2‑140 (MQ=255)
atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                                      <  2:658037/140‑1 (MQ=255)
atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAg                                                                                                                                      <  2:495638/140‑1 (MQ=255)
 tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                    >  2:558378/1‑140 (MQ=255)
 tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAATTTGCTAATCATTTTCGTTCACATCATAGCACCATTCATCAGTGGCTTTTCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                    >  2:157312/1‑140 (MQ=255)
  ttaataGTAGAGCCGCTGATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTa                                                                                                                                    >  2:146383/2‑140 (MQ=255)
  cgatctatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTGACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                   >  2:6591/8‑139 (MQ=255)
  ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                   >  1:374131/1‑139 (MQ=255)
   taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATAATAGCACCAGTTATCAGTGGCTGTGCCAATGCATTTTTTTCTTATTGGCTAAGTAg                                                                                                                                   <  1:28062/140‑1 (MQ=255)
    aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                                                                                 >  1:667840/1‑140 (MQ=255)
     ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCTTTAGTTCTTAAAGCAATTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                                <  1:154182/140‑1 (MQ=255)
     ataCTAGAGTAGCCTATCTCTCAGGCAAAAATT‑‑GAAGAAGAGAGGAGGTGAATTAATTTGAAAAGCATTTTCAGTTGCATCAGATCACCAGTCATCAGTGGCTGTGGTGTTGCGTATGATACTTATTGGCTTAGTAAACg                                                                                                                                <  2:657606/140‑1 (MQ=255)
       agtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTACTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                                                                               <  1:116665/140‑1 (MQ=255)
         tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                                                                                            >  2:353430/1‑139 (MQ=255)
            agacGCCTATCTCTCAGGCGACAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                         <  1:179096/137‑5 (MQ=255)
            agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCATTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                                                                                          >  2:371126/1‑140 (MQ=255)
            agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                                                                                         >  1:644172/1‑136 (MQ=255)
            agTCGCCTACCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTTATCAGTGGCTGTGTCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                                                                                          <  1:173036/140‑1 (MQ=255)
             gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                        >  1:631400/1‑135 (MQ=255)
             gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                        >  1:438656/1‑135 (MQ=255)
             gTCGCCTATATCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                                                                                        >  2:251746/1‑135 (MQ=255)
              tCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑aa                                                                                                                      >  1:284975/1‑140 (MQ=255)
              tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTTCTCAATTGCCGCCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                                       <  1:484475/140‑7 (MQ=255)
              tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                                                                                       >  2:540239/1‑134 (MQ=255)
                gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTTTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACTCAATAC‑‑aa‑‑aa                                                                                                                  >  1:467819/1‑140 (MQ=255)
                gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑aa‑‑aa                                                                                                                  <  2:177297/140‑1 (MQ=255)
                gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                                                                                                     >  1:186500/1‑132 (MQ=255)
                 cctctctttacggCGTAAATTGAGACCCAGAGAGGAGGTGTATAAGGTGTTGGTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGCAATAC‑‑AA‑‑AAt                                                                                                                 <  2:179563/129‑1 (MQ=255)
                 ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AAt                                                                                                                 >  1:264990/1‑140 (MQ=255)
                 ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                    >  1:53523/1‑131 (MQ=255)
                 ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                    >  2:324772/1‑131 (MQ=255)
                 ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                                                                                    >  1:30862/1‑131 (MQ=255)
                  cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                                                                                                   >  2:100951/1‑130 (MQ=255)
                  cTAGCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCCGTCGATGTGCTGTTGCGTATTATACGTATTGGCTTAGTAAACGCaacaaataatta                                                                                                                   <  2:304254/140‑11 (MQ=255)
                   tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTCCTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGATGTGCTGTTGCGTATTATACTTATTAGCTTAGTAAACACaacaagtaattta                                                                                                                  >  1:649359/1‑129 (MQ=255)
                   tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGATGTTGCGTATAATACTTATTGGCTTGGTAAACGCaacaaataattaa                                                                                                                  >  1:451416/1‑129 (MQ=255)
                   tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                                  >  1:418785/1‑129 (MQ=255)
                   tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                                                                                  >  1:307337/1‑129 (MQ=255)
                    aTCTCTCATGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                 <  2:214678/140‑13 (MQ=255)
                    aTCTCTCAGTCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACCTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                 <  2:532574/140‑13 (MQ=255)
                    aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattact                                                                                                                 >  2:624595/1‑128 (MQ=255)
                    aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                                                                                 >  1:314945/1‑128 (MQ=255)
                      ctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTACTTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                                                                                               <  2:660952/140‑15 (MQ=255)
                           aGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACata                                                                                                      >  2:53056/1‑140 (MQ=255)
                                tccaTTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGcc                                                                                                <  2:654316/137‑1 (MQ=255)
                                  aaTTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGc                                                                                             >  2:298503/1‑140 (MQ=255)
                                   aattgacgcctgagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCa                                                                                            <  1:45457/129‑1 (MQ=255)
                                   aTTTAGGCGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCa                                                                                            <  2:117695/140‑1 (MQ=255)
                                     ttAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTTACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCAcc                                                                                          >  2:355254/1‑140 (MQ=255)
                                           gCAGATACGAGTTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATCGGCTAAGTCGCCGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATaaa                                                                                    <  1:657444/140‑1 (MQ=255)
                                             agagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATaaaaa                                                                                  <  1:546191/140‑1 (MQ=255)
                                              gagagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGAACCAATAAAAAt                                                                                 >  1:373733/1‑140 (MQ=255)
                                               agagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATc                                                                                <  1:581282/140‑1 (MQ=255)
                                               agagGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATc                                                                                >  1:442154/1‑140 (MQ=255)
                                                 gggaggGGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCCAAAAAAATcac                                                                              >  2:541456/2‑138 (MQ=255)
                                                 aggaggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCCATAC‑‑AA‑‑AATAGGTGACATATAGCCGCAAACATAAAAATccc                                                                              >  2:228734/1‑140 (MQ=255)
                                                   gaggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                                                                            <  2:375683/140‑1 (MQ=255)
                                                     ggTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCCTTGCGTTTTTTTCTTATTGGCTAAGTAGAGGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCACTAAAAATCCCCCCa                                                                          >  2:293383/1‑140 (MQ=255)
                                                      gtgtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc                                                                         >  1:556422/1‑140 (MQ=255)
                                                       tgtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACt                                                                        <  1:208517/140‑1 (MQ=255)
                                                        gtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTa                                                                       >  1:634105/1‑140 (MQ=255)
                                                        gtATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGAAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCACCTCACTa                                                                       >  1:433186/1‑140 (MQ=255)
                                                        gtATAAGGTGATGCTTATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGAAATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTa                                                                       >  1:354392/1‑140 (MQ=255)
                                                             aaGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGc                                                                   <  1:359776/140‑1 (MQ=255)
                                                              aGGTGATGCTTATTTTCTTTCACATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑CCCCGGTCACAGATAGCCGCACCAATAAAAATCCCCTCACTACCGCa                                                                  <  2:607591/140‑1 (MQ=255)
                                                                 gTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa                                                                >  2:255216/1‑140 (MQ=255)
                                                                 gTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTACGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa                                                                >  1:17989/1‑140 (MQ=255)
                                                                  tgttGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTatttgcggt                                                               <  1:601680/137‑9 (MQ=255)
                                                                        cTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGa                                                          >  1:60442/1‑140 (MQ=255)
                                                                         ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGCCATATAGCCGCACCAATACACAACCCCTCACTACCGCAAGTaacgcg                                                         >  2:552250/1‑135 (MQ=255)
                                                                         ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATCGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATTAAAATCCCCTCACTACCACAAagagagag                                                         >  2:424809/1‑140 (MQ=255)
                                                                          tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        <  2:58478/140‑1 (MQ=255)
                                                                          tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGGTTTGTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        >  1:457144/1‑140 (MQ=255)
                                                                          tATTTCCGTTCCTATTATAGCCCCAGTCATCAGTGGCTGTGCCATTTCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        <  2:355065/140‑1 (MQ=255)
                                                                            ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                                      <  1:130121/140‑1 (MQ=255)
                                                                            ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                                      <  1:290266/140‑1 (MQ=255)
                                                                                  ttCACATCATAGCACCAATCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGgt                                                >  2:25765/1‑140 (MQ=255)
                                                                                        tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCCAAAAGTGAGGGGATTGGTGTATaa                                          <  2:643649/140‑1 (MQ=255)
                                                                                        tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGAATaa                                          >  2:665917/1‑140 (MQ=255)
                                                                                         catAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAg                                         <  1:146383/140‑1 (MQ=255)
                                                                                           tAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGAAAAAATCCCCACACTACTGCAATAGTGTGGGGATTGgtgcat                                            <  1:102347/140‑4 (MQ=255)
                                                                                              cACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCAACAATAAAAATCCCCTCACTACCGCAAATAATGAGGGGATTGGTGTATAAGTAAAt                                    >  1:196179/1‑140 (MQ=255)
                                                                                                   gTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTa                               >  1:562388/1‑140 (MQ=255)
                                                                                                     catcaGTGGCTGCGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGATATATAGCCGCACCAATAAAAATCCCCTCACTAGCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtt                             <  1:53056/140‑1 (MQ=255)
                                                                                                      atcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                            <  1:240119/140‑1 (MQ=255)
                                                                                                      atcaGTGGCTGTGCAATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTATCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                            <  1:343325/140‑1 (MQ=255)
                                                                                                       tcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTATGTAAACGTAATAC‑‑TA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTAttttt                          <  2:467819/140‑2 (MQ=255)
                                                                                                        caGTGGCTGTGCCATTGCGTTTTTTTTTTGTTGGGTGGGAAGACGCAATAC‑‑AA‑‑AAGAGGAGACGTATAGCCGCACCAATGAAAATCCCCTCACTACCGCAAATAGTAGGGGGATTGGTGTATAAGGAAATACTTTTTTTc                          >  2:341920/1‑140 (MQ=255)
                                                                                                        caGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑CA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTc                          >  1:221683/1‑140 (MQ=255)
                                                                                                               tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCt                   >  2:374244/1‑140 (MQ=255)
                                                                                                               tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATACGGAGGGGGTAGGTGTATAAGTAAATACTTTTTTTTGTTTTCt                   >  2:370638/1‑140 (MQ=255)
                                                                                                                 tgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAACACTTATTTTCGTTGTCTTa                 <  1:371126/140‑1 (MQ=255)
                                                                                                                    cATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGAGTATAAGTAAATACTTATTTTCGTTGTCTTAAtt              >  1:611717/1‑140 (MQ=255)
                                                                                                                    cATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAAAAAAAAACCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAAAACTTATTTTCGTTGTCTTAAtt              >  1:596654/1‑140 (MQ=255)
                                                                                                                     aTTGCGTTTTTTTCGTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTTCCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATta             <  1:180334/140‑1 (MQ=255)
                                                                                                                        gCGTTTTTTTCTCTTTGGCTAAGTAGACTCAATAC‑‑AA‑‑AATAGGGCACATATAGCCGCACCAATAAAAATCCCCTCACTTCCGTAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTTTCTTAATTATAc          <  2:43113/140‑1 (MQ=255)
                                                                                                                                ttCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑ATTAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAATTTTTTGCTTTGTTTTATACTGCTAAtt  <  2:642826/140‑1 (MQ=255)
                                                                                                                                ttCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAAtt  <  1:25765/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                      
ATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTATAAGGTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAC‑‑AA‑‑AATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATT  >  CP000730/1929804‑1930068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: