Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT)  murN → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 163.9 / NA 55T15T (ACA→ACT) murNFemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/34);  total (21/34)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  CP000730/2532161‑2532433
                                                                                                                                         |                                                                                                                                       
tgtgTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTtactac                                                                                                                                       >  1:260483/1‑140 (MQ=255)
tgtgTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATGATAGTTCAATTCAGAGAGGGATGAGATAAAATGAACTTTGTAACGTTGACTCCGGATGAGTTCAATGGGTTtactac                                                                                                                                       >  2:282529/1‑140 (MQ=255)
  tgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTActacaa                                                                                                                                     <  1:5153/140‑1 (MQ=255)
         gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                              >  2:145670/1‑140 (MQ=255)
           aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGCGTTCAATGCATTTACTCCAAAGCAttttt                                                                                                                            >  2:79667/1‑140 (MQ=255)
            aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                           >  1:201761/1‑140 (MQ=255)
              cAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcac                                                                                                                         >  1:395152/1‑140 (MQ=255)
               aGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGACCTTTGTAACGTTGACTTTGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcaca                                                                                                                        <  2:59450/140‑1 (MQ=255)
                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTaca                                                                                                                   <  1:274413/140‑1 (MQ=255)
                      gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                 <  1:603863/140‑1 (MQ=255)
                         aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa                                                                                                              <  1:505061/140‑1 (MQ=255)
                          aaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt                                                                                                             <  1:310049/140‑1 (MQ=255)
                                        cATTAGTAATAAATTCAAAAATTATAGTTCAATGCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTAc                                                                                               <  2:336275/140‑1 (MQ=255)
                                         attattAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACAAACAATCAGCAATTCATTACa                                                                                              >  2:216142/1‑140 (MQ=255)
                                          ttattaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACaa                                                                                             <  1:507792/140‑1 (MQ=255)
                                            attaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATc                                                                                           <  1:6280/140‑1 (MQ=255)
                                                 taaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAAtaaaagc                                                                                      >  2:527743/1‑134 (MQ=255)
                                                    aTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAATGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                   <  1:510954/140‑1 (MQ=255)
                                                         aaaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAttt                                                                              >  2:204723/1‑140 (MQ=255)
                                                              tataGTTCAATTCAGAGAGGGATTGGATAAAATTAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAg                                                                         <  1:298261/140‑1 (MQ=255)
                                                              aaaaGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATGACAATCATAGAGTTGATGTAAAAg                                                                         >  2:611223/4‑140 (MQ=255)
                                                               ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTATAAAGTATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAgg                                                                        <  1:33994/140‑1 (MQ=255)
                                                               ataGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAgg                                                                        <  1:250585/140‑1 (MQ=255)
                                                                taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGc                                                                       >  1:14658/1‑140 (MQ=255)
                                                                    tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtg                                                                   <  1:667310/140‑1 (MQ=255)
                                                                       aTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTAACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGCTGTGc                                                                >  2:84204/1‑140 (MQ=255)
                                                                       aTTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTGTGTATGAGGTCAATGCGTTTACTACAAAGCATTTTTCACATTACACATAATCCGATATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGc                                                                <  2:260483/140‑1 (MQ=255)
                                                                           agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCt                                                            <  2:125096/140‑1 (MQ=255)
                                                                            gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                           >  2:502597/1‑140 (MQ=255)
                                                                            gagagGGATGGGATAAAATGAACTTTGTAACGTTGACGTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                           <  2:142702/140‑1 (MQ=255)
                                                                              gagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCAAAATAAAAACAATCAGCTATTCATTACAATCATAGAGATGCTTTAAAAGGCGATGTGCACATTGt                                                         >  1:465625/1‑140 (MQ=255)
                                                                               agGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTa                                                        >  2:561630/1‑140 (MQ=255)
                                                                               agGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCACAGAGTTGATTTAAAAGGCGATGTGCaaccactg                                                        >  2:327069/1‑133 (MQ=255)
                                                                                ggtaggggatAAAATGACTTTTGTAACGGTGACGTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAg                                                       <  2:579240/135‑1 (MQ=255)
                                                                                    tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGTTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  1:279555/140‑1 (MQ=255)
                                                                                    tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  1:479585/140‑1 (MQ=255)
                                                                                     gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCGTCtgtgaggggct                                                  <  2:645265/140‑11 (MQ=255)
                                                                                     gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                  <  2:239181/140‑1 (MQ=255)
                                                                                      ggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTa                                                 <  2:64157/140‑1 (MQ=255)
                                                                                         tAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAg                                              <  2:14658/140‑1 (MQ=255)
                                                                                           aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACTATTAGCAAGACAGAACAATCATAGAGTTGATTTAAAAGGCGATGTCCATCTTGATGGGGTTAAAGAt                                            >  1:154395/1‑140 (MQ=255)
                                                                                            atagAACTTTGTAACGTTGACCTCGGATGAGTTCAATGCGTTTACTACACAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAACAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                           <  2:330595/137‑1 (MQ=255)
                                                                                            aaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGCTTATTGAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                           <  1:458637/140‑1 (MQ=255)
                                                                                            aaTGAACTTTGTAACGTTGACTTCGGATCAATTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCACAGAGTCGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                           <  1:129762/140‑1 (MQ=255)
                                                                                              tGAACTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAATCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGGGCAACTTGTAGGGGTTAAAGATGAc                                         <  1:192937/140‑1 (MQ=255)
                                                                                                            ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                           <  2:651736/140‑1 (MQ=255)
                                                                                                               aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc                        <  2:459511/140‑1 (MQ=255)
                                                                                                                cTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                       <  1:502597/140‑1 (MQ=255)
                                                                                                                  tCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                     >  1:643535/1‑140 (MQ=255)
                                                                                                                  tCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGAGGTAAAAGATGACAATGGTCAAGTGATTGCAgg                     >  1:327053/1‑140 (MQ=255)
                                                                                                                    ggctGAGTTCAATGCGTTTACTATAAAACATTTTTTACATTACAAACAATCAGCTATTTATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                   <  1:344329/137‑1 (MQ=255)
                                                                                                                     gatgctttCAATGCGCTCACTACAAAGCATTATTCATATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                  <  2:625016/134‑1 (MQ=255)
                                                                                                                           ttCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTAATTCCAAACATAGAGTTGATTTAAAAGCCGATGTGCATCATGTAGCGGTTAAAGATGACATTGTTCAAGTGATTGCAGCATGCTTAtt            >  2:185326/1‑140 (MQ=255)
                                                                                                                            tCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTg           >  2:617321/1‑140 (MQ=255)
                                                                                                                            tCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATAATAGAGTAGATTTAAAAGGCGATGTGCATCATGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGCTGCTTATTg           >  1:268400/1‑140 (MQ=255)
                                                                                                                              aaggCGTTTAATACACAGAATTTTCCACATTATACACAATCAGCTATTCATTACAATCATAGAGTTTATTTAAAAGGCGATGTGTCTCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGAc         <  1:226474/137‑1 (MQ=255)
                                                                                                                                    tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGATTATTGCCAGAAga   >  2:351270/1‑139 (MQ=255)
                                                                                                                                     ttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca  <  2:118696/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  CP000730/2532161‑2532433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑