Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,929,875 T→C 66.9% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8750TC66.9% 173.9 / 90.3 134intergenic (‑958/‑210)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/23);  new base C (42/47);  total (64/70)
Fisher's exact test for biased strand distribution p-value = 8.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

TTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGG  >  CP000730/1929736‑1930014
                                                                                                                                                  |                                                                                                                                           
ttCAATCTATAAGCGAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTa                                                                                                                                            >  2:268194/1‑141 (MQ=255)
    aTCTATCAGCTAGAATCATAGAACTTATCAGAAGCAAGTGACTGGGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAGCGCTCAGGCGTCAATTTAGACTCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTAtttt                                                                                                                                        >  2:216176/1‑141 (MQ=255)
      cTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCCATCAGGCGTCAATTTAGACTCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCg                                                                                                                                      <  1:93088/141‑1 (MQ=255)
                  aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAg                                                                                                                          >  2:326067/1‑141 (MQ=255)
                  aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAACAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTGTTTTCGTTGACATCATAg                                                                                                                          >  2:149856/1‑141 (MQ=255)
                   ggatctcagtccacAGATGCAAGTGACTGTGCTCGTATCTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGc                                                                                                                         >  2:86747/14‑141 (MQ=255)
                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCTTAGCACCAGTCATCAGTGGc                                                                                                         <  1:239216/127‑1 (MQ=255)
                                  atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATCGCCCCAGTCATCAGGGGc                                                                                                         >  2:230999/15‑141 (MQ=255)
                                   aaGCAAGTGACTGTGCTTGTATATTTGCCGATCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGATG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                         <  1:264700/141‑1 (MQ=255)
                                     ggaagtcgatatgCTTGTATATTCACAGCTGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTCCT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                      <  1:164810/130‑1 (MQ=255)
                                      gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                     >  1:167636/11‑141 (MQ=255)
                                      gaagtagatatgCTTGTATAATCCCAGACGGTATCATAAAAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGACGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCGCCAGTCATCAGTGGCtgtg                                                                                                     <  1:209770/131‑1 (MQ=255)
                                      gaagtagatatgCTTGTATAATCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAACCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                     >  1:241671/11‑141 (MQ=255)
                                       gagtagatattgttGTACATTCACGGATGGTATAATAATAGAGTCGCCTATCTCTCAGGAGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                                    <  2:351849/129‑1 (MQ=255)
                                       aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                                    <  1:361961/132‑1 (MQ=255)
                                         gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCCCATCCTAGCACCAGTCATCAGTGGCTGTGctg                                                                                                  >  1:158112/8‑139 (MQ=255)
                                         gtaaatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                                  <  2:153668/134‑3 (MQ=255)
                                         gTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGcc                                                                                                   <  1:204145/141‑1 (MQ=255)
                                          tGACCGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑G‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGccc                                                                                                  >  1:269461/1‑140 (MQ=255)
                                           agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCAACAGTCATCAGTGGCTGTGctgtt                                                                                                >  2:18440/6‑137 (MQ=255)
                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                               <  2:175540/137‑6 (MQ=255)
                                            gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                               <  2:372132/137‑6 (MQ=255)
                                              tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                              >  1:262361/1‑141 (MQ=255)
                                                  cTTGTATAGTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                         <  2:21179/141‑4 (MQ=255)
                                                   ttGTATATTCACAGATGGTTTAATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                                        <  2:4917/141‑5 (MQ=255)
                                                    tGTATATTTGCCGATGATATAATAGGAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GC‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                                        <  1:41819/141‑1 (MQ=255)
                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCTTCAGTGGCTGTGCTGTTGCGtattat                                                                                       <  1:375820/141‑6 (MQ=255)
                                                    tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAAGCATATATTT‑‑CTTTAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                       <  1:11342/141‑6 (MQ=255)
                                                      tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt                                                                                      <  1:396339/141‑1 (MQ=255)
                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                     <  2:300593/141‑8 (MQ=255)
                                                      tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                     <  2:170422/141‑8 (MQ=255)
                                                        tattcactgatggtATAATAATAGATTCGCCTATCTCTGAGGCGTCAA‑‑TGGACGAAGAGAGGAGGTGCGT‑TAC‑T‑T‑T‑GC‑TAAGCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt                                                                                   <  1:178478/133‑7 (MQ=255)
                                                         attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                  >  2:327679/8‑141 (MQ=255)
                                                         attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑T‑TACTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                                  >  1:379275/8‑141 (MQ=255)
                                                           ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑CGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAt                                                                                 <  1:60499/141‑1 (MQ=255)
                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGCGCTGTTGCGTATTATACGTATTg                                                                               >  1:210517/5‑141 (MQ=255)
                                                            cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCGGTGCTGTTGCGTATTATCCTTATTg                                                                               >  1:134399/5‑141 (MQ=255)
                                                            cacagatggtATAATAATAGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                               >  1:331669/5‑141 (MQ=255)
                                                            cacagacggaaaaataatAGATTCGCCTATCATTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                               <  1:138513/129‑1 (MQ=255)
                                                             acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGGGCTGTTGCGTATTATACTTATTgg                                                                              <  1:85048/138‑1 (MQ=255)
                                                              cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTGC‑T‑T‑T‑GC‑TAATCATTTTCGTTCAGATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTCTTATACTTATTggt                                                                             >  2:223025/3‑140 (MQ=255)
                                                              cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                             >  1:154708/3‑141 (MQ=255)
                                                                 atggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtta                                                                          >  1:317410/5‑139 (MQ=255)
                                                                  tggtataATCATAGGGCCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                         <  2:173884/138‑4 (MQ=255)
                                                                  tggtagaataatAGAGTCGCTTATCTCTCAGGCGTCAATT‑‑GACGGAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TACTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                         <  2:69275/135‑4 (MQ=255)
                                                                  tgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTTTTGGCTaa                                                                          >  2:63992/1‑141 (MQ=255)
                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGcctagt                                                                        <  2:240761/139‑6 (MQ=255)
                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                        <  1:198195/139‑1 (MQ=255)
                                                                   ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                        <  1:117363/139‑1 (MQ=255)
                                                                   ggtataATAATAGAGTCGCCTATCGCTCAGTCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                        <  2:276286/139‑1 (MQ=255)
                                                                   gatATAATAGTAGAGTCGCCTATCTCTCAGGCGACAATTTAGACGCAGAGAGGAGGGG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCATCTTGGCTAAg                                                                         >  2:211056/1‑141 (MQ=255)
                                                                    gtataATAATAGAGTCGCCTATCCCTCAGGCGCCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                       <  1:755/140‑1 (MQ=255)
                                                                     tataATAGTAGAGTCGCCTATCTCTTAGGCGTAAATTTAGACGCAGAGAGGAGGGG‑TG‑TAA‑G‑G‑G‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGtt                                                                       >  2:273363/1‑140 (MQ=255)
                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTAGCTGTGCTGTTGCGTATGATACTTATTGGCTGAGTaa                                                                      >  2:236414/1‑140 (MQ=255)
                                                                     tataATAATAGAGTCGCCTATCTCTCAGGCGTAAATT‑‑GACGAAGAGCGGAGGTG‑CA‑TTA‑G‑T‑TAGT‑TACTCATTTTGGCTTATATCATAGAACCAGTTCTCAGTGGCTGTGTGTTTGCTGTTTATACTTATTGGCTTAGTaa                                                                      >  2:329658/1‑140 (MQ=255)
                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACTAAGAGAGGAGGTG‑CA‑TTTCT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                     <  1:178075/141‑3 (MQ=255)
                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGCAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGGATTATACTTATTGGCTTAGTaaa                                                                     >  2:86108/1‑139 (MQ=255)
                                                                      ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                     >  1:27226/1‑139 (MQ=255)
                                                                      ataataataGAGTCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGGGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                     <  1:99730/141‑3 (MQ=255)
                                                                         ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAA‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                  >  1:32389/5‑141 (MQ=255)
                                                                         ataataGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                  <  1:107586/141‑1 (MQ=255)
                                                                         ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAGTGGCTTAGTAAACGc                                                                  <  1:294771/141‑1 (MQ=255)
                                                                         agaagagcggcGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCAGCAGTGGCTTTGCCGTTGCTTATTATACTTATTGGCTTAGTAAACGc                                                                  <  2:143877/131‑1 (MQ=255)
                                                                          tagtagAGTCGCCGATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                  <  1:147474/141‑1 (MQ=255)
                                                                          atgtagAGTCGTCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGc                                                                  <  1:267220/139‑1 (MQ=255)
                                                                           aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                >  1:69935/3‑141 (MQ=255)
                                                                           aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                <  1:59507/139‑1 (MQ=255)
                                                                           aatagAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCCCCCATTAGTAGTGGCTGGTTTCTTTCGTATTATACTTATTGGCTTAGTAAACGCaa                                                                <  1:92973/139‑1 (MQ=255)
                                                                            atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                               <  1:61452/140‑2 (MQ=255)
                                                                              agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                             <  2:360076/141‑4 (MQ=255)
                                                                              agagTCCCCTATTTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                             <  2:10224/141‑4 (MQ=255)
                                                                               gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCGCCGGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                            >  1:389272/1‑137 (MQ=255)
                                                                                agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                                            <  1:370019/141‑1 (MQ=255)
                                                                                agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                           <  2:175061/141‑6 (MQ=255)
                                                                                agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                           <  1:228455/141‑6 (MQ=255)
                                                                                   cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GT‑TAATCATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTGAGTCAACGCaacaaataat                                                        <  1:294894/141‑9 (MQ=255)
                                                                                   cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                                        >  1:32827/1‑133 (MQ=255)
                                                                                    gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAGGCaacaaataatt                                                       >  2:223362/1‑132 (MQ=255)
                                                                                    gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGccacaaataatt                                                       >  2:128686/1‑130 (MQ=255)
                                                                                     ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGGAGCAATACCAAATa                                                       >  2:350998/1‑141 (MQ=255)
                                                                                     ccTATCTCTCAGGCATCAAATTAGAAGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATa                                                       >  1:216671/1‑141 (MQ=255)
                                                                                      cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                     <  2:367696/141‑12 (MQ=255)
                                                                                       tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                    <  2:315908/141‑13 (MQ=255)
                                                                                       tATCTCTCAGGCGTCAACT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                                    <  1:359212/141‑13 (MQ=255)
                                                                                        aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCCCTATTAGCTTAGTAAACGaaacaaataattaata                                                   >  1:304464/1‑128 (MQ=255)
                                                                                        aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                   >  2:349620/1‑128 (MQ=255)
                                                                                        aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                   >  1:149819/1‑128 (MQ=255)
                                                                                        aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattcatc                                                   >  2:293934/1‑128 (MQ=255)
                                                                                        aTCTCTCAGGCGTCAATT‑‑GAAGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATAATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                                   >  2:121391/1‑128 (MQ=255)
                                                                                        aTCTCTCAGGCGTCAATT‑‑GAAGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑T‑TACTAATCATTTTCGCTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGTaacaaataattaata                                                   >  1:240248/1‑128 (MQ=255)
                                                                                         tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                                  <  1:323452/141‑15 (MQ=255)
                                                                                              cAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                              <  1:273782/141‑1 (MQ=255)
                                                                                               aGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACatat                                             <  2:262361/141‑1 (MQ=255)
                                                                                                ggCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata                                            >  1:159331/1‑141 (MQ=255)
                                                                                                 gCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAg                                           <  1:335541/141‑1 (MQ=255)
                                                                                                    ccAATGTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCg                                        <  2:170324/140‑1 (MQ=255)
                                                                                                             aCGCAGAGGTGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaa                               <  2:220766/141‑1 (MQ=255)
                                                                                                             aCGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaa                               <  2:287627/141‑1 (MQ=255)
                                                                                                                    gaggagGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATccc                        <  1:86747/141‑1 (MQ=255)
                                                                                                                                                cTTATTTTCGTTCATATCATAGCACCAGTCATCAGTGGCTGTGCCATCGCGTTTTTTTATTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGTACCCATAAAAATCCCCTCACTACCGCAAATAGTGAg   <  2:101414/141‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
TTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGG  >  CP000730/1929736‑1930014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑