Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,963,768 C→G 100% V365L (GTA→CTA)  hemG ← protoporphyrinogen oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,963,7680CG100.0% 142.8 / NA 44V365L (GTA→CTA) hemGprotoporphyrinogen oxidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/18);  total (26/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGT  >  CP000730/1963636‑1963902
                                                                                                                                    |                                                                                                                                      
tCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCAc                                                                                                                                >  2:366436/1‑141 (MQ=255)
      aaCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCa                                                                                                                          >  1:344372/1‑141 (MQ=255)
       aCGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGACCGAATCaa                                                                                                                         >  1:322675/1‑141 (MQ=255)
              aCAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAacctta                                                                                                                  <  1:123342/141‑1 (MQ=255)
                aaTTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacc                                                                                                                <  1:245681/141‑1 (MQ=255)
                 aTTGTAAATTCAGTATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacct                                                                                                               <  1:276529/141‑1 (MQ=255)
                  ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACTTGGTATACCTAGATACGCACGAATAAAAaacttacatt                                                                                                              >  1:151337/1‑141 (MQ=255)
                  ttGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAaccttacctt                                                                                                              <  1:355875/141‑1 (MQ=255)
                         ttCAGGATCACCTTTCAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCAGTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgtgt                                                                                                       >  1:294896/1‑141 (MQ=255)
                         gtCAGGATCACCTTTAACTGTCATCATTTGACTTAAACCTCTACGTACAATCGATACTAATTCATTATCGGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGgtgt                                                                                                       <  2:113023/140‑1 (MQ=255)
                             ggATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACNTGGTTTACCTAGATACGCACGNANCAAAANCNTACCTTCCGGTggagta                                                                                                   >  1:320741/1‑141 (MQ=255)
                                  aCCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTGGTAAATgg                                                                                              >  1:131013/1‑141 (MQ=255)
                                   ccTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAACCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGc                                                                                             <  2:303749/141‑1 (MQ=255)
                                       tAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGATTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCAtt                                                                                         >  1:335131/1‑141 (MQ=255)
                                                     tgatttatgtcttTACGTACGATCGTTAGTAATTCGTTATCTGTATGATTATCATCCTCTGTATCAGCTGGTTTACCTAGTGACGCACGAATCGAGTCCTTACCTTCGGGTGTAGTAAATGGCCATTTTTTCGATGTCCaa                                                                           <  2:23843/132‑1 (MQ=255)
                                                      gACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAg                                                                          <  1:113237/141‑1 (MQ=255)
                                                                  tACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTaa                                                              >  1:126923/1‑141 (MQ=255)
                                                                   aCGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAAt                                                             <  1:236402/141‑1 (MQ=255)
                                                                       aCAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACTTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTGATGTCt                                                         >  2:19562/1‑141 (MQ=255)
                                                                        cAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCCAGTACATGCGGTAATGTCTg                                                        <  1:241435/141‑1 (MQ=255)
                                                                               tACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTGGAATGTCCAAGTACATGCGGTAATGTCTGTATCAcc                                                 >  1:268045/1‑140 (MQ=255)
                                                                                   aaTTCATTATCTGTATGATCATCAACCACAGTCTCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGtt                                             <  1:264661/141‑1 (MQ=255)
                                                                                    aTTCATTATCTGTATGATCATCAACCACAGTATCACGTGGTTTACCTAGATACGTACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGCTGTCCAAGTACATGCGGTTATCTCTGTATCACTCGTtc                                            <  1:221299/141‑1 (MQ=255)
                                                                                          tATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATTTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCaa                                      >  1:371550/1‑141 (MQ=255)
                                                                                           aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAt                                     >  2:77510/1‑141 (MQ=255)
                                                                                           aTCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAAt                                     <  1:173350/141‑1 (MQ=255)
                                                                                             cTGTATGATCAGCAACCACTGTATGACCTGGTTTACCTAGACACGGACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCCGTAAGGTCTGTATCACTCGTTCTCGCAATTa                                   <  2:26700/141‑1 (MQ=255)
                                                                                              tGTATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTAc                                  >  1:129742/1‑141 (MQ=255)
                                                                                                tATGATCATCAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGa                                >  2:371996/1‑141 (MQ=255)
                                                                                                    atcatcAACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGcc                            >  1:50964/1‑141 (MQ=255)
                                                                                                      catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGCTGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTTCGAAGCCAg                          >  1:338010/1‑141 (MQ=255)
                                                                                                      catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg                          >  2:85467/1‑141 (MQ=255)
                                                                                                      catcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAg                          >  1:64594/1‑141 (MQ=255)
                                                                                                       atcaACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGACGTAAATGGCCATTTTTTCGATGTCAAAGTACATGCGGTAATGCTCGTATCCCTCGTTTTCGCAATTGCGAAGCCCGt                         >  2:105917/1‑141 (MQ=255)
                                                                                                         caACCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAACTGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATCTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTAc                       <  2:294896/141‑1 (MQ=255)
                                                                                                           aCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACca                     >  2:185011/1‑141 (MQ=255)
                                                                                                           aCCACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACca                     >  2:245913/1‑141 (MQ=255)
                                                                                                            ccACAGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTACCGAGTATTTAATGGCCATTTTTTCTTTGTCTAAGTAGATGCGGTAATGTCTGTATCCCTCGTTCTCGCAATTACGAAGCCAGTAGcat                    >  2:83346/1‑141 (MQ=255)
                                                                                                               caGTATCACCTGGTTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCTCAATTACGAAGCCCGTACcatcat                 >  1:244767/1‑141 (MQ=255)
                                                                                                               caGTATCACCTGGGTTACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACcatcat                 <  2:284769/141‑1 (MQ=255)
                                                                                                                 gTATCACCTGGTTTACATAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATgg               >  1:220917/1‑141 (MQ=255)
                                                                                                                      cccTCGTTTACCTAGATACGCACGAATCAAACCCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTCTTGTCTATATCACGCGTTCTCCGAATTACGAAGCCAGTACCATCATGGGTAtt          <  2:180875/140‑1 (MQ=255)
                                                                                                                      aCCTGTTTTACCTGGATACGCACGCATCAAAACCTTGCCTTCCGGTGTAGTACATGGCCATTTTTTCGATGTCCAAGGACATGCGGTCATGCCTGTATCACTCGTTCTCGCAATTACGTAGCCAGTACCATCATGGGTAtt          <  2:230934/141‑1 (MQ=255)
                                                                                                                              tACCTAGATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGt  >  2:149047/1‑141 (MQ=255)
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TCGGCAAACGATTGACAATTGTAAATTCAGGATCACCTTTAAATGTCATCATTTGACTTAAATCTCTACGTACAATCGATACTAATTCATTATCTGTATGATCATCAACCACAGTATCACCTGGTTTACCTACATACGCACGAATCAAAACCTTACCTTCCGGTGTAGTAAATGGCCATTTTTTCGATGTCCAAGTACATGCGGTAATGTCTGTATCACTCGTTCTCGCAATTACGAAGCCAGTACCATCATGGGTATTTTCAATGT  >  CP000730/1963636‑1963902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: