| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,279,705 | G→A | 100% | intergenic (+1373/+6659) | mtlD → / ← glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,279,705 | 0 | G | A | 100.0% | 114.3 / ‑6.5 | 41 | intergenic (+1373/+6659) | mtlD/glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/18); minor base T (0/1); total (22/19) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT > CP000730/2279567‑2279841 | gTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACt < 2:129141/141‑1 (MQ=255) ttattaGTTGATTCTACTTCTGGTTTAATAGTTACACCGTTCTCCATCGTAGGCCTGTTTGTTGATGACAATCCGTTAGGAAGGTGTAAT‑GCGTGCGTTTTATCAGTGGCATCATTAGCTTCTGTTGTTACTGTTGTTTCa > 2:329752/1‑141 (MQ=255) taGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg > 2:333242/1‑141 (MQ=255) taGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg > 1:82411/1‑141 (MQ=255) taGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTg > 1:82408/1‑141 (MQ=255) taGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCGGATGTTACGGTTGTTTCACCTg > 1:82412/1‑141 (MQ=255) ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 2:270143/141‑1 (MQ=255) ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 1:356917/141‑1 (MQ=255) tGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc > 1:138310/1‑141 (MQ=255) cTTCAGGTTTACTAGTTACATCGTTATCCAATGTCGGACTGTTTGTTGATGCACCTACACTCGAATTGT‑TATTAGCTTGCGGTTTATCATTAGCATCATCAGTTCCTGATGTTACTGTTGTTTCACCTTTTGCCGGATccg > 2:240921/1‑139 (MQ=255) tgggTTTACTAGTTACGTTGTTTTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat < 2:303368/139‑1 (MQ=255) aTCGTTATCCATTGTTCGACTGTTTGTTGATGCATCTACACTATAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg < 1:221241/141‑1 (MQ=255) aTCCATTGTCGGCCTGTTTGTTGCTGCATCTACACTAGTATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGc < 1:18967/141‑1 (MQ=255) ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TGTTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATGACTATTGTTTGGTGTTGTCGGAGAAGCGt > 2:201138/1‑141 (MQ=255) cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc < 1:123947/141‑1 (MQ=255) ggACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCAACTGCATCATCAGGTGCTGATGTTACTGTTGTTTCACATGTTGCCGCATCACTATTGTTTGGTGTTGTCGGAGACGCGTCTGCTTTg > 2:98821/1‑141 (MQ=255) ttgttgATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg < 1:324794/141‑1 (MQ=255) ttgttgAGGCATCGACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg < 1:320258/141‑1 (MQ=255) tttgATGCATCTACATCAGAATTGC‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTTTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGTGAAGCGTCTGCTTTGCCATTAGCt < 1:288454/140‑1 (MQ=255) aTCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtctc < 2:248160/141‑1 (MQ=255) tCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa > 1:216665/1‑141 (MQ=255) cTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAg > 2:315692/1‑141 (MQ=255) tACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa > 1:226438/1‑141 (MQ=255) cTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGGAGCATCAGTTGCTGATGTTACTGGGGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACg > 2:180726/1‑141 (MQ=255) cTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACg > 2:77545/1‑141 (MQ=255) gT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTg > 2:215702/1‑141 (MQ=255) taGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg < 1:159444/141‑1 (MQ=255) gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg < 2:216665/141‑1 (MQ=255) tttaggaTTTGCATCATCAGTTGGTGCTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGGTAGGTGGTCCAGTATTTGCTgg > 2:282554/7‑141 (MQ=255) ttATCATTTGCATTATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt < 2:138310/141‑1 (MQ=255) aTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgtcca > 2:194236/1‑138 (MQ=255) tttGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGATTTGGCATGAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCa > 1:388240/1‑141 (MQ=255) gCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAttt > 1:262154/1‑140 (MQ=255) atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGGTGTTGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATAGGTTGGGTTGTCCAGTATTTTTTGTTGTtgcatttgcatt > 2:109439/1‑141 (MQ=255) tcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGCAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt > 2:90751/1‑141 (MQ=255) cacttGCTGATGTTACTGTGGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTTGCTGTCGTTTCAGATACGTTCGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTg < 1:61758/138‑1 (MQ=255) tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAtt > 1:52778/1‑141 (MQ=255) cTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTg < 2:32418/141‑1 (MQ=255) aTGTTTCTTTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg < 2:180112/141‑1 (MQ=255) aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg < 2:248454/141‑1 (MQ=255) tGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt < 1:333242/141‑1 (MQ=255) | GTTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGT‑TATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT > CP000730/2279567‑2279841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |