Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,784,861 C→T 10.5% T309T (ACG→ACA)  aur ← M04 family aureolysin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,784,8610CT10.5% 94.6 / 3.1 38T309T (ACG→ACA) aurM04 family aureolysin
Reads supporting (aligned to +/- strand):  ref base C (14/20);  new base T (2/2);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TGTGTTAATTCGTGTGCTACTACGTCATTTGCACCCGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCTTTTACGAAGTTTTCAT  >  CP000730/2784721‑2784997
                                                                                                                                            |                                                                                                                                        
tgtgtTAATTCGTGTGCTACTACGTCATTTGCACCCGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGc                                                                                                                                          >  2:207548/1‑141 (MQ=255)
tgtgtTAATTCGTGTGCTACTACGTCATTTGCACCCGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGc                                                                                                                                          >  1:224790/1‑141 (MQ=255)
     tAATTCGTGTGCTACTACGTCATTTGCACCCGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCTTTATTTCTGTTATCTTGACCACCGCAGTTCTTAACATGCGTTaa                                                                                                                                     <  1:216309/141‑1 (MQ=255)
       aTTCGTGTGCTACTACGACATTTGCACCCGATAAACTTGTGAATGTGCGACCATCATCATCACCATCGATCATTTTGTCACCGAGCCATGCGGCATTATTTCTGTTATCTTGACCACTGTAGTTATTAACATGCGTTAATg                                                                                                                                   <  1:83671/141‑1 (MQ=255)
                       gTCATTTGCACCCGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTTTCTTGACCACCCTAGTTATTAACTTGCGTTAATGAAATCTTTGGATTAcc                                                                                                                   >  2:222482/1‑141 (MQ=255)
                            ttGTACCCGTTTAACTTGTGAATTGGCTACCATCACCTGCACCATAGATCATTTTGTCACCGATCCATGCGGTATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACAAccaaggt                                                                                                              <  1:277194/141‑6 (MQ=255)
                            ttGCACCCGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATTCGTTCATTGAACAATTTGCCTCTCTTGGt                                                                                                              >  2:152220/1‑141 (MQ=255)
                                   cGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCata                                                                                                       <  2:337391/141‑1 (MQ=255)
                                   cGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCata                                                                                                       <  2:209843/141‑1 (MQ=255)
                                           ttGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCAc                                                                                               <  2:251808/141‑1 (MQ=255)
                                             gtgAATGTGCGACCATCACCATCACCAGAGATCATTTTGTCACCGATCCATGCGGCATTATTTGTGTTATCTTGACCACCGTAGTTATTACCATGCTGTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGa                                                                                             >  2:188/1‑141 (MQ=255)
                                              tgAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGAc                                                                                            >  1:281206/1‑141 (MQ=255)
                                                   gtgCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAAt                                                                                       <  2:245110/141‑1 (MQ=255)
                                                    tgCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAAtg                                                                                      <  2:32957/141‑1 (MQ=255)
                                                                            tCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATAtgttt                                                              <  1:32937/141‑1 (MQ=255)
                                                                                 ttGTCACTGATCCATGCGGCATTATTTCTGTTATCTTGCCCACCGTAGTTATTAACATTCGTTAATGAAACAATTGGACTACCTTGGTTGTCATCTGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTa                                                         >  2:23780/1‑141 (MQ=255)
                                                                                  tGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTATGTAATAATCATATgtttgttttc                                                        >  2:63063/1‑139 (MQ=255)
                                                                                   gTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATTTTTGTTTAGc                                                       >  2:24018/1‑141 (MQ=255)
                                                                                   gTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGc                                                       >  1:176691/1‑141 (MQ=255)
                                                                                   gTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTGGTTGAGc                                                       >  1:285327/1‑141 (MQ=255)
                                                                                      aCCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGta                                                    >  1:116036/1‑141 (MQ=255)
                                                                                            ccATGCGGCATTATTTCTGTTATTTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAAtt                                              <  1:105992/141‑1 (MQ=255)
                                                                                             cATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGTGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGCCTTTGTAATAATCATATGTTTGTTTAGCGTAATAAttt                                             >  2:72405/1‑141 (MQ=255)
                                                                                             cATGCGGCATCATTTCTGTTATCTTGACCACCGTAGTTATTAACATGTGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAAttt                                             >  2:267302/1‑141 (MQ=255)
                                                                                                  ggCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATc                                        <  1:342579/141‑1 (MQ=255)
                                                                                                     attattTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTAc                                     <  2:165106/141‑1 (MQ=255)
                                                                                                                 aTCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTg                         <  2:121407/141‑1 (MQ=255)
                                                                                                                   cTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCt                       <  1:207548/141‑1 (MQ=255)
                                                                                                                   cTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCt                       <  2:224790/141‑1 (MQ=255)
                                                                                                                    ttGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTc                      <  1:146671/141‑1 (MQ=255)
                                                                                                                    ttGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATACGTTTGTTTAGCGTAATAAGTTGCATCTACTCCAGCACGTTGCTc                      <  1:216280/141‑1 (MQ=255)
                                                                                                                      gACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCAt                    >  2:142135/1‑141 (MQ=255)
                                                                                                                       accatctttgTTATTAACATGCGTTAATGAAACAATTGGACTACGTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATc                   <  2:73241/132‑1 (MQ=255)
                                                                                                                        ccaccGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCt                  >  1:13616/1‑141 (MQ=255)
                                                                                                                               aGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCTTTTACGa           >  1:214016/1‑141 (MQ=255)
                                                                                                                               aGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCTTTTACGa           >  1:190894/1‑141 (MQ=255)
                                                                                                                                   attaACATGCGTTAATGAAAGAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCTTTTACGAAGtt       <  2:32514/141‑1 (MQ=255)
                                                                                                                                       aCATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCTTTTACGAAGTTTTCa   <  1:142135/141‑1 (MQ=255)
                                                                                                                                        cATGTGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCTTTTACGAAGTTTTCAt  <  1:267302/141‑1 (MQ=255)
                                                                                                                                        cATGTGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCTTTTACGAAGTTTTCAt  <  1:72405/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
TGTGTTAATTCGTGTGCTACTACGTCATTTGCACCCGATAAACTTGTGAATGTGCGACCATCACCATCACCATAGATCATTTTGTCACCGATCCATGCGGCATTATTTCTGTTATCTTGACCACCGTAGTTATTAACATGCGTTAATGAAACAATTGGACTACCTTGGTTGTCATATGATTCACGACCAAATGTGTCTTTGTAATAATCATATGTTTGTTTAGCGTAATAATTTGCATCTACGCCAGCACGTTGCTCATCTTTTACGAAGTTTTCAT  >  CP000730/2784721‑2784997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑