Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,479	T→C	30.4%	T25A (ACA→GCA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,479	0	T	C	30.4%	75.8 / 30.2	55	T25A (ACA→GCA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base T (13/25);  new base C (9/8);  total (22/33)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATG  >  CP000730/36340‑36615                                                                                                                                            |                                                                                                                                         taataaGCTTTTTTATGCCTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTATACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                                                                                         <  1:92299/141‑1 (MQ=255)    taaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTatat                                                                                                                                      <  2:336082/141‑1 (MQ=40)       gCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCtt                                                                                                                                   >  1:60057/1‑141 (MQ=38)           ttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAt                                                                                                                               <  1:337162/141‑1 (MQ=35)            ttttTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGCACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                                                                                                                              <  1:152762/141‑1 (MQ=25)            tttATGTTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                                                                                                                              <  1:152820/141‑1 (MQ=25)            tttATGCGTTTTCTTCCAAGTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTATTCCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                                                                                                                              <  1:152768/141‑1 (MQ=255)               aTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagta                                                                                                                           >  1:237534/1‑141 (MQ=35)                         ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCt                                                                                                                 <  1:54437/141‑1 (MQ=35)                         ttcCAAATTGGAGACAAAATTGGGGCCTATTCTTGAACCCAACGGGAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCt                                                                                                                 <  2:168775/141‑1 (MQ=255)                              aaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGt                                                                                                            <  2:155771/141‑1 (MQ=34)                              aaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGt                                                                                                            <  2:155579/141‑1 (MQ=35)                                      aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTg                                                                                                           >  1:301000/1‑134 (MQ=33)                                      aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTg                                                                                                           <  2:301000/134‑1 (MQ=34)                                       cAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATc                                                                                                   >  2:149589/1‑141 (MQ=33)                                           aTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                               <  2:337420/141‑1 (MQ=32)                                             tGGGGCATATTCTTGAACCCACCGGTAGACCGTTGAATGATGACCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGtt                                                                                             <  1:174269/141‑1 (MQ=12)                                                ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                                                                                          >  2:274496/1‑141 (MQ=32)                                                ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAAATCCTTGTTaaa                                                                                          >  2:314020/1‑141 (MQ=17)                                                     atTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGtt                                                                                     >  2:224855/1‑141 (MQ=32)                                                        cTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTtat                                                                                  <  2:161697/141‑1 (MQ=32)                                                            aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                              <  2:60057/141‑1 (MQ=32)                                                                         aCCGTTGAATGATGAAAGCTTAGACCAGGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATa                                                                 >  1:393003/1‑141 (MQ=255)                                                                             ttcaaTGAGGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACaga                                                             <  2:376735/138‑1 (MQ=17)                                                                                    atgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTcc                                                      <  1:314492/141‑1 (MQ=32)                                                                                         cGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttttt                                                 <  2:237534/141‑1 (MQ=17)                                                                                                 ccacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTACATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAt                                         >  2:269971/1‑141 (MQ=17)                                                                                                 ccacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAt                                         >  2:60889/1‑141 (MQ=32)                                                                                                  cacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATCCAGAAGACTCCTTTTTGTTAAAAtt                                        >  2:364218/1‑141 (MQ=17)                                                                                                             aaTATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAAt                             <  2:120899/141‑1 (MQ=21)                                                                                                                atTTCAGTTATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                          <  2:341181/141‑1 (MQ=25)                                                                                                                aattCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                          <  2:313371/139‑1 (MQ=25)                                                                                                                          tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTACAAc                >  2:390579/1‑141 (MQ=25)                                                                                                                          aatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                <  1:334007/140‑1 (MQ=25)                                                                                                                            ttacaatatCTGAATGCATATCTTAGATAATAGCCAACTGCTACAGTTATAACATCCTTGTTAAATTTTTTATATCTGAAATAGTTCATACATAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAg              <  1:132207/136‑1 (MQ=1)                                                                                                                                  tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACATTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt        >  2:231374/4‑141 (MQ=9)                                                                                                                                  tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt        <  1:114297/138‑1 (MQ=9)                                                                                                                                  tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt        <  2:266808/138‑1 (MQ=9)                                                                                                                                  tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt        <  1:341964/138‑1 (MQ=12)                                                                                                                                  tAACTCAATGTATATCTTAGATAGTAGCCAACGTCTACAGTCATAACATACTATTTAAATTGTTTACATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt        <  2:295754/141‑1 (MQ=11)                                                                                                                                  tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt        <  2:312671/141‑1 (MQ=37)                                                                                                                                  tAACTCAATGTATATCTTAGATAGTAGACAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAGACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt        <  2:391034/141‑1 (MQ=255)                                                                                                                                  aaaCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt        <  2:245004/140‑1 (MQ=37)                                                                                                                                     gtCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt     >  2:102875/2‑141 (MQ=16)                                                                                                                                     cgcAATGCGTATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt     <  2:187865/139‑1 (MQ=255)                                                                                                                                     cTCAATGCATATCTTAGATAGTCGCCAACGGCTACAGTGATAACATCCTTGTTAAGTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACAGtatt     >  2:288262/1‑141 (MQ=11)                                                                                                                                     cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGCAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt     >  1:150085/1‑141 (MQ=12)                                                                                                                                     cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAACTTATAGTATAAATTCAACTTTGCAACATAACCGtgat     >  1:138063/1‑138 (MQ=11)                                                                                                                                      tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt    >  1:26978/1‑140 (MQ=12)                                                                                                                                       cAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtattat   >  1:25919/1‑141 (MQ=40)                                                                                                                                       cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt   >  1:32293/1‑139 (MQ=12)                                                                                                                                       cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTCAAAATTAGATTATAAATTCAACTTTGCAACAGAACCGtatttt   <  1:21100/141‑3 (MQ=11)                                                                                                                                       cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTAAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTTCAACAGAACCGtatttt   >  1:261939/1‑139 (MQ=11)                                                                                                                                        aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATTCTTGTTAAATTGTTTATAGCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttta  <  1:380664/141‑4 (MQ=11)                                                                                                                                        aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatttta  >  2:321788/1‑138 (MQ=21)                                                                                                                                        aaTGCATATCGTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTACATTGTTTGTAACTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGATCCGtatttta  <  1:91112/141‑4 (MQ=11)                                                                                                                                            |                                                                                                                                         TAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATG  >  CP000730/36340‑36615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑