Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,501	G→A	10.7%	A17A (GCC→GCT)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,501	0	G	A	10.7%	152.8 / 9.4	77	A17A (GCC→GCT)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base G (27/39);  new base A (3/5);  total (30/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

TTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACA  >  CP000730/36364‑36635                                                                                                                                          |                                                                                                                                       ttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCt                                                                                                                                     <  1:54437/141‑1 (MQ=35) ttcCAAATTGGAGACAAAATTGGGGCCTATTCTTGAACCCAACGGGAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCt                                                                                                                                     <  2:168775/141‑1 (MQ=255)      aaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGt                                                                                                                                <  2:155579/141‑1 (MQ=35)      aaTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGt                                                                                                                                <  2:155771/141‑1 (MQ=34)              aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTg                                                                                                                               >  1:301000/1‑134 (MQ=33)              aCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTg                                                                                                                               <  2:301000/134‑1 (MQ=34)               cAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATc                                                                                                                       >  2:149589/1‑141 (MQ=33)                   aTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTg                                                                                                                   <  2:337420/141‑1 (MQ=32)                     tGGGGCATATTCTTGAACCCACCGGTAGACCGTTGAATGATGACCGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGtt                                                                                                                 <  1:174269/141‑1 (MQ=12)                        ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                                                                                                              >  2:274496/1‑141 (MQ=32)                        ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAAATCCTTGTTaaa                                                                                                              >  2:314020/1‑141 (MQ=17)                             atTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGtt                                                                                                         >  2:224855/1‑141 (MQ=32)                                cTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTtat                                                                                                      <  2:161697/141‑1 (MQ=32)                                    aaCCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCt                                                                                                  <  2:60057/141‑1 (MQ=32)                                                 aCCGTTGAATGATGAAAGCTTAGACCAGGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATa                                                                                     >  1:393003/1‑141 (MQ=255)                                                     ttcaaTGAGGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACaga                                                                                 <  2:376735/138‑1 (MQ=17)                                                            atgaACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTcc                                                                          <  1:314492/141‑1 (MQ=32)                                                                 cGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCttttt                                                                     <  2:237534/141‑1 (MQ=17)                                                                         ccacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTACATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAt                                                             >  2:269971/1‑141 (MQ=17)                                                                         ccacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAt                                                             >  2:60889/1‑141 (MQ=32)                                                                          cacGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATCCAGAAGACTCCTTTTTGTTAAAAtt                                                            >  2:364218/1‑141 (MQ=17)                                                                                     aaTATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAAt                                                 <  2:120899/141‑1 (MQ=21)                                                                                        atTTCAGTTATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                              <  2:341181/141‑1 (MQ=25)                                                                                        aattCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                              <  2:313371/139‑1 (MQ=25)                                                                                                  tatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTACAAc                                    >  2:390579/1‑141 (MQ=25)                                                                                                  aatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                    <  1:334007/140‑1 (MQ=25)                                                                                                    ttacaatatCTGAATGCATATCTTAGATAATAGCCAACTGCTACAGTTATAACATCCTTGTTAAATTTTTTATATCTGAAATAGTTCATACATAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAg                                  <  1:132207/136‑1 (MQ=1)                                                                                                          tagcccaaggcacatCTTAGATAGTAGCCAACGGCTACAGTTATAACATCCTTGTTAAATTTTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                            <  2:255695/128‑1 (MQ=11)                                                                                                          tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACATTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                            >  2:231374/4‑141 (MQ=9)                                                                                                          tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                            <  1:114297/138‑1 (MQ=9)                                                                                                          tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                            <  1:341964/138‑1 (MQ=12)                                                                                                          tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                            <  2:266808/138‑1 (MQ=9)                                                                                                          tAACTCAATGTATATCTTAGATAGTAGCCAACGTCTACAGTCATAACATACTATTTAAATTGTTTACATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                            <  2:295754/141‑1 (MQ=11)                                                                                                          tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                            <  2:312671/141‑1 (MQ=37)                                                                                                          tAACTCAATGTATATCTTAGATAGTAGACAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAGACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                            <  2:391034/141‑1 (MQ=255)                                                                                                          atactcgaggcatatCTTAGATAGTAGACAGCAGCTACAGTGCTAACATACTTGTTAAATTGTTTATATCTGAAATAGTTCACACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCACCAGAAcctc                            <  2:335425/130‑3 (MQ=11)                                                                                                          aaaCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                            <  2:245004/140‑1 (MQ=37)                                                                                                             gtCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                         >  2:102875/2‑141 (MQ=16)                                                                                                             cgcAATGCGTATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                         <  2:187865/139‑1 (MQ=255)                                                                                                             cTCAATGCATATCTTAGATAGTCGCCAACGGCTACAGTGATAACATCCTTGTTAAGTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACAGtatt                         >  2:288262/1‑141 (MQ=11)                                                                                                             cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGCAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                         >  1:150085/1‑141 (MQ=12)                                                                                                             cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAACTTATAGTATAAATTCAACTTTGCAACATAACCGtgat                         >  1:138063/1‑138 (MQ=11)                                                                                                              tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                        >  1:26978/1‑140 (MQ=12)                                                                                                               cAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtattat                       >  1:25919/1‑141 (MQ=40)                                                                                                               cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttt                       >  1:32293/1‑139 (MQ=12)                                                                                                               cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTCAAAATTAGATTATAAATTCAACTTTGCAACAGAACCGtatttt                       <  1:21100/141‑3 (MQ=11)                                                                                                               cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTAAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTTCAACAGAACCGtatttt                       >  1:261939/1‑139 (MQ=11)                                                                                                                aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATTCTTGTTAAATTGTTTATAGCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttta                      <  1:380664/141‑4 (MQ=11)                                                                                                                aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatttta                      >  2:321788/1‑138 (MQ=21)                                                                                                                aaTGCATATCGTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTACATTGTTTGTAACTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGATCCGtatttta                      <  1:91112/141‑4 (MQ=11)                                                                                                                 atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttat                     >  1:151237/5‑137 (MQ=255)                                                                                                                  tgcatatCTTAGATAGTAGCCAACTGCTACAGTGATAACATCCTTGTTAATTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                    <  2:258476/138‑6 (MQ=11)                                                                                                                   tcatatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTTAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactat                   <  2:227239/139‑12 (MQ=11)                                                                                                                   gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                   <  1:84353/139‑7 (MQ=255)                                                                                                                   gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                   <  2:103847/139‑7 (MQ=255)                                                                                                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                  <  1:132482/140‑13 (MQ=11)                                                                                                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                  <  1:38526/140‑8 (MQ=255)                                                                                                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                  >  1:100155/2‑134 (MQ=255)                                                                                                                    catatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcctttcatata                  >  1:346257/2‑129 (MQ=11)                                                                                                                    catatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactata                  <  1:298743/140‑13 (MQ=11)                                                                                                                    catatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGATAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactata                  >  2:216056/2‑129 (MQ=11)                                                                                                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagt                 >  1:181274/1‑133 (MQ=255)                                                                                                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAAGTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatt                 >  1:329376/1‑128 (MQ=11)                                                                                                                      tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                >  1:120056/1‑127 (MQ=11)                                                                                                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                >  1:335379/1‑127 (MQ=11)                                                                                                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                <  2:198132/141‑15 (MQ=11)                                                                                                                       attttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATaga               <  2:335219/138‑1 (MQ=255)                                                                                                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAATTAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACGTTGCAACGGAACCGtattttatgagtac               <  1:256203/141‑11 (MQ=11)                                                                                                                        tCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaca              <  2:172975/141‑12 (MQ=255)                                                                                                                        tCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaca              <  2:29210/141‑12 (MQ=11)                                                                                                                        tCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGCTTATATCGGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCTtattttatgagtaca              <  1:291933/141‑12 (MQ=11)                                                                                                                        tCTTAGATAGTAGCCAACCGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtaca              <  1:284944/141‑12 (MQ=11)                                                                                                                          ttAGATAGTAGCCAGCGGCTACAGTGATAACATCTTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGAt            <  2:224247/141‑1 (MQ=255)                                                                                                                          ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacact            <  2:157853/141‑14 (MQ=11)                                                                                                                                 gtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTa     >  1:361194/1‑141 (MQ=255)                                                                                                                                   agCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATGCCACGTTGCAACAGAACCGAATTATGGAATAGACATGTTGGTAAc   >  1:246424/1‑141 (MQ=17)                                                                                                                                    gCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACa  <  1:60889/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACA  >  CP000730/36364‑36635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑