Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,915 G→A 15.5% intergenic (+973/‑161) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,9150GA15.5% 77.2 / 3.1 36intergenic (+973/‑161)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base G (22/8);  new base A (3/3);  total (25/11)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATC  >  CP000730/76786‑77054
                                                                                                                                 |                                                                                                                                            
agggagtGAATGTATTTACGCTCTATATAAAAAGAACCGTAGGTCTCTTCAGATCTACGGATTTTCCCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAtt                                                                                                                                  <  2:46769/135‑1 (MQ=1)
       gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCCCGAAATTAGCATCATGCTAGCAGGTTAGGAGGACAGTGACATGCCAGATTGGTGGTTAGGTATGTTTTTTTAc                                                                                                                           >  1:81609/4‑141 (MQ=1)
       gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTAc                                                                                                                           <  1:324005/138‑1 (MQ=1)
       gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTAc                                                                                                                           <  1:235921/138‑1 (MQ=1)
       gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAttttttact                                                                                                                           >  2:136963/4‑138 (MQ=1)
       gagtGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATATACGGATATTCGCCATGCCACGAAATTAGCATCACGCTAGCAAGTTAAACGAACACTGACAAGATATATTAGTGATTAGCTATAttttttact                                                                                                                           >  2:158045/4‑138 (MQ=1)
       gagtGTATTTAAGCTCTATATAAAAAGAACCGCAGGGCTCTTCAGATCTACGGATTTTCGACATGACACGAAATTAGCATCATGCTAGCAAGTTAAACGAACACTGACATGATATATTAGTGATTAGCTATAttttttact                                                                                                                           >  2:134238/4‑138 (MQ=1)
            tagttACGCTCTATATAAAAAGAACCGTAGGTCCTTTCAGCGCTACGGATTTTGCCCATGCCACGAAAGTAGCATCAGTCTAGCAAGTTAAACGAACACTGACATGATATATTATTGATTAGCTATATTTTTTACTTTGCa                                                                                                                     <  2:140875/138‑1 (MQ=1)
            tactgactctctATATAAAAAGAACCGCAGATCTCTTCAGCTCTAAGGATTTTCGCCAGGGCCGGAAATTAGCATCATGGTTGCCAGTTATGCGAACACTGACCTGATACATTAGTGGTTAGCTATATGTATTTACttttc                                                                                                                      >  2:68896/9‑139 (MQ=0)
                              aaaGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTACTTTGCAACAGAACCTATAAAAAAc                                                                                                   >  2:40229/1‑141 (MQ=37)
                                  aaCCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCGTGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTGTTTACTTTGCAACAGAACCTATAAAAAACATg                                                                                                >  1:264309/1‑141 (MQ=255)
                                      gCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTAAAGCGAAAACTGACATTATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCt                                                                                            >  2:43107/1‑141 (MQ=255)
                                       cAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGACAACTCACCTAATAAATAAGTGGTTAGCTATATTTTTTTCATTTGCACCAGAACCTCTAAAAAAAATGGGCtt                                                                                           >  1:80750/1‑141 (MQ=14)
                                        aGGTCTCTTCAGATCTACGGATTTTCGCAATACCCCGAAATTCGAATGGAGCTAGCAAGTTAAGCGAACACTGACATGATAGATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTa                                                                                          >  1:333172/1‑141 (MQ=14)
                                         ggTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTaa                                                                                         >  1:176127/1‑141 (MQ=255)
                                          gTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGATATATTTTTTTACTTTGCAACAGAACCTATAAACAACAGAGGCTTaaa                                                                                        >  2:166001/1‑141 (MQ=255)
                                            ctctTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTaaaag                                                                                      <  1:254349/141‑3 (MQ=255)
                                                                      tGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCaaa                                                            >  1:193461/1‑141 (MQ=255)
                                                                      tGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCaaa                                                            >  1:193445/1‑141 (MQ=255)
                                                                        ccACGAAATTAGCATCATGCTAGCAAGTTAAGACAACACTGACCTGGTTAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACa                                                          <  1:268310/141‑1 (MQ=21)
                                                                         cACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAAGTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACAt                                                         >  1:33858/1‑141 (MQ=255)
                                                                           cGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTCATTTATATAGTATTACTGCAAACATTg                                                       >  1:117944/1‑141 (MQ=255)
                                                                                  aGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTggg                                                >  1:278679/1‑141 (MQ=255)
                                                                                        acgCTAGCCAATCAAGCGAACACTGACATGATAAATTAGTGGTTAGCTTTATGTGTTTACTTTGCAACAGAATCTATAAACAACATGGGTTTTAATGGTTTATTTAGTTAGTATTACCTCAAACATTGATTTGGGTCaaaa                                          <  2:88509/139‑1 (MQ=18)
                                                                                         tGCTAGCAAGTTAAGCGAACACTGAAATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCaaaaa                                         <  2:48018/141‑1 (MQ=255)
                                                                                          gCTAGCAAGTTAAGCGAACACTGACATGATCAATTAGTGATTAGCTACAGTGTTTTACGCGGCGACGCAGCCTATAGAGAACATTTTCGTAAAAGGTTAAGTGATATATTATTACTGCAAACATTGATTTGGGTCAAAAAt                                        <  2:390794/141‑1 (MQ=12)
                                                                                             aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATGGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg                                     >  1:368884/1‑141 (MQ=255)
                                                                                             aGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAg                                     >  1:49416/1‑141 (MQ=255)
                                                                                                     aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTAGTGCAAACATTCATTTCGGTCAAAAATTAGGGGTATTa                             >  2:185399/1‑141 (MQ=255)
                                                                                                     aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                             >  2:302435/1‑141 (MQ=255)
                                                                                                     aaGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTa                             >  2:147598/1‑141 (MQ=255)
                                                                                                       gCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaa                           >  1:209897/1‑141 (MQ=255)
                                                                                                          aaCACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTaaaaaa                        <  2:190859/141‑1 (MQ=255)
                                                                                                                        aaaTTAGTGGTTAGCTATATCTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCAt           <  1:347734/141‑1 (MQ=255)
                                                                                                                        aaaTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAGAAATTAGGGGTATTAAAAAAATGAATGAATCATa          >  1:368486/1‑141 (MQ=255)
                                                                                                                          aTTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAAt        >  1:209270/1‑141 (MQ=255)
                                                                                                                                ggTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAAGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATc  <  2:193445/141‑1 (MQ=255)
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AGGTATTGAATGTATTTACGCTCTATATAAAAAGAACCGCAGGTCTCTTCAGATCTACGGATTTTCGCCATGCCACGAAATTAGCATCATGCTAGCAAGTTAAGCGAACACTGACATGATAAATTAGTGGTTAGCTATATTTTTTTACTTTGCAACAGAACCTATAAAAAACATGGGCTTAAAGGGTTAATTTATATAGTATTACTGCAAACATTGATTTGGGTCAAAAATTAGGGGTATTAAAAAAATGAATGAATCATAATTTTATC  >  CP000730/76786‑77054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: