Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 129,796 G→C 100% intergenic (‑173/+212) spa ← / ← sarH1 immunoglobulin G binding protein A/staphylococcal accessory regulator H1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730129,7960GC100.0% 160.2 / NA 49intergenic (‑173/+212)spa/sarH1immunoglobulin G binding protein A/staphylococcal accessory regulator H1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/28);  total (21/28)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TATTTATAATTAAATTTAAAGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGAGATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGTTTTAAGTGATATAGAAAATAGAAAGTTGTT  >  CP000730/129660‑129928
                                                                                                                                        |                                                                                                                                    
tatTTATAATTAAATTTAAAGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACatat                                                                                                                                  >  1:373180/1‑141 (MQ=255)
 atTTATAATTAAATTTAAAGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGCAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATAtt                                                                                                                                 >  1:59108/1‑141 (MQ=255)
 atTTATAATGAAATTTAAAGGTAAGTTTTACGACTTATAACATAAATATTTTGCTGAAAGATTTATACAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAAGAAAAACAGATAAAATATAGACatact                                                                                                                                 >  1:290519/1‑139 (MQ=255)
        aTTAAATTTAAAGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTtata                                                                                                                          <  1:275385/141‑1 (MQ=255)
        aTTAAATTTAAAGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTAGATTAATAAAAACAGATAAAATATAGACATATTTTTtata                                                                                                                          <  2:120550/141‑1 (MQ=255)
           aaaTTTAAAGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTGTTATAATg                                                                                                                       >  1:720/1‑141 (MQ=255)
            aagttAAAGGTAAGTTTTACAACTTATAAAAAAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAAGCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGa                                                                                                                      <  2:101571/138‑1 (MQ=255)
               ttAAAGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAg                                                                                                                   <  1:85/141‑1 (MQ=255)
                  aaGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCgg                                                                                                                >  1:393544/1‑141 (MQ=255)
                                aaCATATAAAATAAATAGTTTGCTGAAAGATTAATTCATTATGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGa                                                                                                  <  1:304925/141‑1 (MQ=255)
                                   ttATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTACGATTAATAAAAACAGATAAAATATAGACCTATTTTTTATAATGAAAGCGGTTTAATACATATGAGtt                                                                                               <  1:335536/141‑1 (MQ=255)
                                            aaataTTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGAGGTTTAATACATATGAGTTAAAAAatat                                                                                      >  2:199320/1‑141 (MQ=255)
                                            aaataTTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATAACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAatat                                                                                      >  2:362308/1‑141 (MQ=255)
                                                atTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATAttttt                                                                                  <  2:327619/141‑1 (MQ=255)
                                                  tttGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTaa                                                                                <  2:8795/141‑1 (MQ=255)
                                                         aaaGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATaaa                                                                         <  1:102886/141‑1 (MQ=255)
                                                          aaGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATTTTTAATGCGGTATAATACATACGAGTTAAAAAATATTTTTAAGATTAAAg                                                                        >  2:85893/1‑141 (MQ=255)
                                                                taatCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGc                                                                  <  2:32707/138‑1 (MQ=255)
                                                                tATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGc                                                                  >  2:314333/1‑141 (MQ=255)
                                                                 aTTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCt                                                                 >  2:96039/1‑141 (MQ=255)
                                                                 aTTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGACt                                                                 >  1:161582/1‑141 (MQ=255)
                                                                 aTTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATACGAGTTAAAAACTATTTTTAAGCATAAAGTGGGGCt                                                                 >  1:276395/1‑141 (MQ=255)
                                                                  ttCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCtt                                                                >  2:347824/1‑141 (MQ=255)
                                                                        aaGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAAtg                                                          <  1:389631/141‑1 (MQ=255)
                                                                          gTAAAACGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAAtgtg                                                        <  1:167500/141‑1 (MQ=255)
                                                                              aaGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGa                                                    >  1:159766/1‑141 (MQ=255)
                                                                                ggCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAgg                                                  >  1:351479/1‑141 (MQ=255)
                                                                                         ccGCAAAATCACGCTATTTCGATTAATAAAAACTGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTc                                         <  2:220287/141‑1 (MQ=255)
                                                                                               aaTCATGCTATTTCGATTAATAAAAACAGATAAAATATAGACAAATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAAt                                   <  1:12707/141‑1 (MQ=255)
                                                                                               aaTCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAAt                                   <  1:145318/141‑1 (MQ=255)
                                                                                                attactttATTTCGATTAATAACAAAAGATAAAACATAGACCTATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATg                                  <  2:160825/134‑1 (MQ=255)
                                                                                                aTCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATg                                  <  1:370003/141‑1 (MQ=255)
                                                                                                 tCACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTCATACATATGAGTTAAAAAATATTTTTACGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTGCGGAAATgg                                 >  2:327209/1‑141 (MQ=255)
                                                                                                 acACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATgg                                 <  2:309972/140‑1 (MQ=255)
                                                                                                  cACGCTATTTTGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGt                                <  2:95550/141‑1 (MQ=255)
                                                                                                  cACGCTATTTCGATTAATAAAAACAGATCAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGt                                <  1:274387/141‑1 (MQ=255)
                                                                                                  cACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGt                                <  1:153638/141‑1 (MQ=255)
                                                                                                  cACGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGt                                >  1:280822/1‑141 (MQ=255)
                                                                                                   aCGCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGtt                               >  1:333644/1‑141 (MQ=255)
                                                                                                     gCTATTTCGATTAATAAAAACAGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGtttt                             <  2:131554/141‑1 (MQ=255)
                                                                                                             gATTAATAAAAACAGATAAGATATAGACATATTTTATATAATGAAAGCTGTTTAATAAAAAAAAAAAAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTGAATTTCGGAAATGGTTTTAAGTGata                     <  2:237484/141‑1 (MQ=255)
                                                                                                             aaTTAAAAAAAACAGATAAAATAGAGACATATTTTTTATAATGAAAGCGGTTTAATACATATAAAATAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGTTTTAAGTGata                     <  2:306554/140‑1 (MQ=255)
                                                                                                                          aGATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGGGTGAAGTGATatagaaaatagaaa        <  2:280822/141‑1 (MQ=255)
                                                                                                                          aGATAAAATATAGACATATTTTGTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGTTTTAAGTGATatagaaaatagaaa        <  2:62394/141‑1 (MQ=255)
                                                                                                                          aGATAAAATATAGACATATTGTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGTTTTAAGTGATatagaaaatagaaa        >  1:240730/1‑141 (MQ=255)
                                                                                                                           gATAAAATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGTTTTAAGTGATATAGAAAATAGAAAg       <  2:371711/141‑1 (MQ=255)
                                                                                                                           cctAATATATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGTTTTAAGTGATATAGAAAATAGAAAg       <  1:284112/139‑1 (MQ=255)
                                                                                                                               aaaTATAGACATATTTTTTATAATGAAAACGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGTTTTAAGTGATATAGAAAATAGAAAgttgt   >  2:280444/1‑141 (MQ=255)
                                                                                                                                aaTATAGACATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGGTGAGGTTTAATTTCGGAAATGGTTTTAAGTGATATAGAAAATAGAAAgttgtt  >  1:145460/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TATTTATAATTAAATTTAAAGGTAAGTTTTACAACTTATAAAATAAATATTTTGCTGAAAGATTTATTCAGGAAGTAAAAGGCTTAAAACCGCAAAATCACGCTATTTCGATTAATAAAAACAGATAAAATATAGAGATATTTTTTATAATGAAAGCGGTTTAATACATATGAGTTAAAAAATATTTTTAAGAATAAAGTGGGGCTTTGAATGTGCTGAGGTTTAATTTCGGAAATGGTTTTAAGTGATATAGAAAATAGAAAGTTGTT  >  CP000730/129660‑129928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: