| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 618,567 | T→C | 19.7% | D1223D (GAT→GAC) | sdrD → | Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 618,567 | 0 | T | C | 19.7% | 67.3 / 4.5 | 39 | D1223D (GAT→GAC) | sdrD | Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrD |
| Reads supporting (aligned to +/- strand): ref base T (16/15); new base C (6/2); total (22/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACT > CP000730/618427‑618706 | gATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagat > 1:127351/1‑141 (MQ=255) tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc < 1:266514/141‑1 (MQ=255) gcgactcagattcagacagcgactcagattcagacaGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACCCAGACAGCGACTCAGACTCAGATAGCGACtcagatcccg > 2:282267/1‑137 (MQ=255) ttcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGAGTCAGATGTCGACTCAGACCCAGATAGTGACTCAGATTCAGATAGCGACTc > 1:170655/1‑141 (MQ=17) acagcgtgactcagattcagacacccactcagaTTCGGACTGTTATTCAGACTCAGACGGCCACTCAGATTCAGACCGCGCCTCAGACTCAGACACCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGa < 2:127351/134‑1 (MQ=2) gcgactcagattcagacagcgactcagaCTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGGTTCAGATAGCGACTCAGAATCgg > 2:186301/1‑141 (MQ=255) actcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGAATCGGACa > 2:103811/1‑141 (MQ=255) actcagattcagacagcgactcagaCTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACGCAGATTCGGAcg > 2:272344/1‑140 (MQ=255) agattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGa < 1:61699/141‑1 (MQ=255) cgtcatctagcgtctcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCCCCTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAtt < 2:128285/133‑1 (MQ=14) tccacacgacacagaCTCCGAACGAGAATTAGAATCAGAAAGACACTCAGAATCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa < 2:127790/135‑1 (MQ=14) gacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGCCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGAc < 1:27182/141‑1 (MQ=255) tcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGACTCAGACTCAGATAGCGACTCAGACTCGGAAGGCGAGTCAGAccactatttc > 2:376944/1‑132 (MQ=11) ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCTGTCATCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc < 1:287361/141‑1 (MQ=255) ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc < 1:4637/141‑1 (MQ=255) ctcagaTAGAGAATCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc < 1:383549/141‑1 (MQ=255) cagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcag < 1:57312/141‑1 (MQ=255) agaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga > 1:114495/1‑141 (MQ=255) agaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACACCGACTCAGACTCAGATGGCGACccaga > 2:185956/1‑141 (MQ=11) cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTAAGACTCGGCTAGCGATTCAGAATCAGATAGCGACTCAGATTCAGAGAGCg > 2:25286/1‑141 (MQ=14) aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGAGTCAGATAGTGACTCAGACTCAGGTAGGGAGTCAGATTCTGAAAGCGAATCAGACTCAGAGAGCGAGACAGAGTCAGATGGCggag > 2:87398/1‑138 (MQ=11) aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACGGCGACTCAGACTCTGATAGTGACTCAGATTCGGAGAGCGCCTCAGATTCGGACAGAGATTAAGACTGAGGTAGCGACTCAGGGTAAGGTAGCGaga > 2:150555/1‑139 (MQ=14) cTCAGATAGCGACTCACATTCAGACAGCGACTCAGACTCAGACACCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTc < 2:206469/141‑1 (MQ=255) gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGAATCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGAGTCGGACAGCGATTCAGACTCAGATATCGACTCAGATTCAGATAGCGATTCGGAc > 1:224374/1‑141 (MQ=255) gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCCACTCAGAATCAGATAGCGACTCACAAACAGACAGCGAGTCGGAccccaaa > 2:5856/1‑135 (MQ=11) aGATTCAGACAGCGACTCAGACTCAGACTGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCTGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa < 1:224030/141‑1 (MQ=255) aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa < 1:92273/141‑1 (MQ=255) aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACt > 1:325081/1‑141 (MQ=255) aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGACTCGGACACCGATTCAGACTCACATAGCGACGCAGATTCAGATAGCGATACGGACTCAGATAGCGAcc > 2:154317/1‑140 (MQ=17) aTTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACCCAGATTCAGATAGCGATTCAGACTCAGATAACGACTCAGACTCAGATAGCGATGCAGACTCAGAGAACGAcc > 2:193451/1‑140 (MQ=11) caaggactcagactcagacagggactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc < 2:8610/137‑1 (MQ=255) ctcagactcagacagcgacccagactcggaTAGTGACGCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg < 1:263227/141‑1 (MQ=255) cagactcagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACCCAGATTCGGCCAGCGAATCAGACTCAAATAGCGACTCAGATTCAGATAACGATTCGGACTCAGATAGCGCCTCACATTCAGAAAgcg > 2:7618/1‑139 (MQ=14) cagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAg > 1:325184/1‑141 (MQ=255) gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAc > 1:176831/1‑141 (MQ=255) cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGCCTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa < 2:114495/141‑1 (MQ=255) ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg > 1:384027/1‑141 (MQ=255) tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGc > 1:136000/1‑141 (MQ=255) tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAACGATTCGGACTCCGATAGCGACTCAAACTCAGAAAGCGATTCCGACGCAGACAGc > 2:17994/1‑141 (MQ=14) tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATGCAGATAGCGATTCAGACGCAGATAGCGACCCAGATTCCGCTAGTGATTCAGACACAGATAGc > 2:90882/1‑141 (MQ=14) actcagaTTGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACt < 1:231854/141‑1 (MQ=255) cgatccggACGCAGACTCAGACAGCGACTCAGATTCTGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGa < 2:256933/134‑1 (MQ=11) gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAGTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAATCAGATTCAGACAGCGAc > 1:187066/1‑141 (MQ=255) attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATCGCGACTCAGAACCAGCCAGCGAcc > 2:16859/1‑140 (MQ=255) attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGGCt > 1:300874/1‑141 (MQ=255) | GATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACT > CP000730/618427‑618706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |