Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	673,010	T→C	12.1%	G78G (GGT→GGC)	yvrC →	iron (Fe3+) ABC superfamily ATP binding cassette transporter, binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	673,010	0	T	C	12.1%	74.5 / 2.7	33	G78G (GGT→GGC)	yvrC	iron (Fe3+) ABC superfamily ATP binding cassette transporter, binding protein
Reads supporting (aligned to +/- strand):  ref base T (16/13);  new base C (2/2);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

GGCTCGTCTAAATCGCCGTACCATAGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTAGAGAAACAAGGCATCAAAGT  >  CP000730/672873‑673147                                                                                                                                           |                                                                                                                                          ggCTCGTCTAAATCGCCGTACCATAGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAg                                                                                                                                        <  1:2842/141‑1 (MQ=255)     cGTCTAAATCGCCGTACCATAGAATTGTTTCGTTAATGCCAAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑CGGTGTTTCAACGGTTGATGATGATCCAAACGATGTGAAAAAGGGTAAGAAAc                                                                                                                                    >  2:248324/1‑141 (MQ=255)       tCTAAATAGCCAAACAATAAAATTGTTTCGTTAATGTCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACACAGT‑TGGTGTTTCAACGGTTGATGATTATCAAAAAAAAAAAAAAAAGGGTAAGAAACaa                                                                                                                                  <  2:118012/141‑1 (MQ=255)              cGCCGTACCATAGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATg                                                                                                                           <  2:238254/141‑1 (MQ=255)              cGCCGGACCATAGAATTGTTTAGTAAATGGCTATTAATACTCAAATTTTAGATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATg                                                                                                                           <  1:150083/141‑1 (MQ=255)                    actaaaGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGCAAGAAACAATTTGATGCTTTGa                                                                                                                     >  2:102642/6‑141 (MQ=255)                    aCCATAGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGCAAGAAACAATTTGATGCTTTGa                                                                                                                     >  2:225439/1‑141 (MQ=255)                    aCCATAGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGAGTAAGAAAGAATTTGATGCTGTGa                                                                                                                     >  1:221740/1‑141 (MQ=255)                                               gTAATACTGAAATTTTATATGAATTAGGATTAGGTAATTACATTGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGAAACTTTTCAAgg                                                                                          <  1:278826/141‑1 (MQ=255)                                               gTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAgg                                                                                          <  1:254923/141‑1 (MQ=255)                                                 aaTATTGAAATTTTATATGAATTAGGATTTGGTAAATACAGTGG‑TGGTGTTTGAACGGTTGATGATTATTCAAAAAAAGAGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCa                                                                                        <  2:203063/141‑1 (MQ=255)                                                              tataTGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTaa                                                                           <  1:44498/141‑1 (MQ=255)                                                               atatGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCACAGCCAGATCTAAt                                                                          >  1:10489/1‑141 (MQ=255)                                                               atatGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAAt                                                                          >  1:203849/1‑141 (MQ=255)                                                                          gcttaggTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATg                                                               >  2:46835/3‑141 (MQ=255)                                                                           attaggTAAA‑ACCTATTCTGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGa                                                              >  2:183975/1‑141 (MQ=255)                                                                                  aaaTACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTCAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCaa                                                       >  1:388316/1‑141 (MQ=255)                                                                                  aaaTACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTAGCaa                                                       >  2:137381/1‑141 (MQ=255)                                                                                        aTAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCa                                                 >  1:280995/1‑141 (MQ=255)                                                                                                gtgtTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCta                                          <  1:201292/141‑1 (MQ=255)                                                                                                gtgtTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCta                                          >  2:222481/1‑141 (MQ=255)                                                                                                 tgtTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGCAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCtaa                                         <  1:225439/141‑1 (MQ=255)                                                                                                 tgtTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGCAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCtaa                                         <  1:102642/141‑1 (MQ=255)                                                                                                    ttCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCtaataa                                      >  2:17548/1‑141 (MQ=255)                                                                                                      cAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAg                                    >  1:100285/1‑141 (MQ=255)                                                                                                        aCGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTa                                  >  2:270481/1‑141 (MQ=255)                                                                                                              gatgatTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGtca                            <  2:203849/141‑1 (MQ=255)                                                                                                               atgatTAGCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGtcat                           <  2:315267/141‑1 (MQ=255)                                                                                                                     aTCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTag                     >  1:18057/1‑141 (MQ=255)                                                                                                                     aTCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTaa                     >  2:99584/1‑140 (MQ=255)                                                                                                                              aTGTGAAAAAGGGTAAGAAAAAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTAGACAAACAAg            <  1:248324/141‑1 (MQ=255)                                                                                                                                       aGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATGAGAGAAACAAGGCATCAAag   >  1:367248/1‑141 (MQ=255)                                                                                                                                        gggTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTAGAGAAACAAGGCATCAAagt  <  1:222481/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                          GGCTCGTCTAAATCGCCGTACCATAGAATTGTTTCGTTAATGCCTAGTAATACTGAAATTTTATATGAATTAGGATTAGGTAAATACATAGT‑TGGTGTTTCAACGGTTGATGATTATCCAAAAGATGTGAAAAAGGGTAAGAAACAATTTGATGCTTTGAATCTAAATAAAGAGGAACTTTTAAAGGCAAAGCCAGATCTAATTCTTGCGCATGAGTCGCAAAAGGCAACTGCTAATAAAGTATTGTCATCATTAGAGAAACAAGGCATCAAAGT  >  CP000730/672873‑673147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑