Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,499,376 C→A 100% G883V (GGT→GTT)  ebh ← extracellular matrix binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,499,3760CA100.0% 108.7 / NA 37G883V (GGT→GTT) ebhextracellular matrix binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/22);  total (15/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGT  >  CP000730/1499240‑1499509
                                                                                                                                        |                                                                                                                                     
gCCTCGATAGTATTTGGCATTTATTCCAACAGGAATATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCGTTACTCATATTAGATGCAACTACTTTATTATAATAAATTACTGCTCTAATTTGTTTAACTAc                                                                                                                                   >  1:161621/1‑141 (MQ=255)
 catCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACa                                                                                                                                  <  2:245545/139‑1 (MQ=255)
   tCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAgg                                                                                                                                <  1:245648/141‑1 (MQ=255)
     gATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGATAGGATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtg                                                                                                                              <  1:91216/141‑1 (MQ=255)
       tAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAggtggt                                                                                                                            >  1:246659/1‑141 (MQ=255)
        attATTTGGCATTTATCCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTa                                                                                                                           <  1:195505/139‑1 (MQ=255)
                   tttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATacaaca                                                                                                                <  1:57229/141‑1 (MQ=255)
                    ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACAt                                                                                                               <  2:165130/141‑1 (MQ=255)
                    ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACAt                                                                                                               <  1:63933/141‑1 (MQ=255)
                    ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACAt                                                                                                               <  1:103728/141‑1 (MQ=255)
                    ttATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCAACGCTTCTTAACTACCGGTGGTATGAATACAACAt                                                                                                               <  2:363237/141‑1 (MQ=255)
                        tCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATc                                                                                                           <  1:35373/141‑1 (MQ=255)
                         ccAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCt                                                                                                          <  1:365476/141‑1 (MQ=255)
                            aCAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACGACATAATCTTGc                                                                                                       >  2:222262/1‑141 (MQ=255)
                            aCAGGAGTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCCCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTgg                                                                                                       >  2:16126/1‑140 (MQ=255)
                              cggATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTAGAGGTGGTATGAATACAACATAATCTCGCgg                                                                                                     <  2:241500/140‑1 (MQ=255)
                                  ttattGCTTGTTGTTTCAATATTATCTGCCAAAATAGTAACAGCATTACTCCTACTACAGCCAACTACTTTATTATAATAAACTACTGCTCTAATTTTTTTAACTACAGGTGGTATGAATACAACAGAATCTTGCGGTCGt                                                                                                 <  1:90854/141‑1 (MQ=255)
                                  ttattGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGt                                                                                                 <  1:107909/141‑1 (MQ=255)
                                    attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACCGGTGGTATGAATACAACATAATCTTGCGGTCGtat                                                                                               >  2:7279/1‑141 (MQ=255)
                                    attgattgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGtat                                                                                               >  2:135707/1‑141 (MQ=255)
                                        attgTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTa                                                                                           <  2:192231/141‑1 (MQ=255)
                                              ggtggAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTACTATAATAAACTACTGCCCTAATTGGTTTAACTACAGGTGGTATGAATACAACATAATCTTCCGGTCGTATAGTAACTGta                                                                                     >  2:250560/1‑141 (MQ=255)
                                                tggAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGtatt                                                                                   >  2:172582/1‑141 (MQ=255)
                                                 ggAATGTCATCTGGCAAAATCGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGtatta                                                                                  >  1:94887/1‑141 (MQ=255)
                                                  gAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGtattat                                                                                 <  1:344128/141‑1 (MQ=255)
                                                             ggCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCa                                                                      <  1:42131/141‑1 (MQ=255)
                                                                          cAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCg                                                         <  1:214785/141‑1 (MQ=255)
                                                                          cAGCATTACTCATATTAGATGCAACCACTTTATCATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATACACCCCATCAGGTGTACCAGTCg                                                         <  1:304991/141‑1 (MQ=255)
                                                                           aggaTTACTGAAGTTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTAGGCACCCCATCAGGTGTACCAGTCGc                                                        <  2:3805/138‑1 (MQ=255)
                                                                                            aTGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTTCCAGTCGCAAGTATCGTATAACCAg                                       >  1:218676/1‑141 (MQ=255)
                                                                                              gCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGAt                                     >  1:155852/1‑141 (MQ=255)
                                                                                                 actactTTATTATAATAAACTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGt                                  >  1:12224/1‑141 (MQ=255)
                                                                                                                  aaCTACTGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATCGTATTTGATCTGCCTCACt                 >  1:325568/1‑141 (MQ=255)
                                                                                                                    ctactGCTCTATTTTGTTTAACTACAGGTTGTTTGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCCGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTac               <  2:188133/141‑1 (MQ=255)
                                                                                                                        tGCTCTAATTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTaccacc           >  2:66121/1‑141 (MQ=255)
                                                                                                                              aaTTTGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTACCGTATAACCAGATGGCATTTGATCTGCCCCACTCCCACCATCCGt     >  2:196978/1‑141 (MQ=255)
                                                                                                                                 ttGTTTAACTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGt  <  2:94887/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
GCCTCGATAGTATTTGGCATTTATTCCAACAGGATTATTGATTGTTGGTGGAATGTCATCTGGCAAAATAGTAACAGCATTACTCATATTAGATGCAACTACTTTATTATAATAAACTACTGCTCTAATTTGTTTACCTACAGGTGGTATGAATACAACATAATCTTGCGGTCGTATAGTAATTGTATTATGCACCCCATCAGGTGTACCAGTCGCAAGTATCGTATAACCAGATGGTATTTGATCTGCCTCACTACCACCATTCGTAGT  >  CP000730/1499240‑1499509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: