Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,624,673 T→A 100% K192I (AAA→ATA)  malA ← alpha‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,624,6730TA100.0% 71.7 / ‑6.5 27K192I (AAA→ATA) malAalpha‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (12/14);  minor base G (1/0);  total (13/14)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTTTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATCC  >  CP000730/1624539‑1624809
                                                                                                                                      |                                                                                                                                        
tttGTATACCTGGCCGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCaa                                                                                                                                    >  2:394738/1‑141 (MQ=255)
  tGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTATTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAac                                                                                                                                  >  1:247508/1‑141 (MQ=255)
    tataCCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCGTCTACTCTAAATCCATCAATGCCTATATCAAaccc                                                                                                                                >  1:304006/1‑140 (MQ=255)
    tataCCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAacca                                                                                                                                <  1:264291/141‑1 (MQ=255)
     ataCCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTTCCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCATTGCCTGTATCAAaccac                                                                                                                               >  2:255761/1‑141 (MQ=255)
             cTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTtcat                                                                                                                       >  2:297363/1‑141 (MQ=255)
                aTTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTcatcat                                                                                                                    >  1:79106/1‑141 (MQ=255)
                       tCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAAt                                                                                                             <  1:161773/141‑1 (MQ=255)
                               aaaTGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATTCCTATATCAAACCACCAGTTCATCATTTCAAATACAGAAtc                                                                                                     >  1:30468/1‑141 (MQ=255)
                                              tGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAATTGATCATTTCAAATACAGCATCTCTAACTTCCGGAtt                                                                                      >  1:169967/1‑141 (MQ=255)
                                                     cccTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCaa                                                                               >  2:293135/1‑141 (MQ=255)
                                                                 ggtaAGTTACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCTTTGCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGgt                                                                   <  1:352090/141‑1 (MQ=255)
                                                                   taAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGgttg                                                                 <  1:183672/141‑1 (MQ=255)
                                                                   taAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAAGCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGgttg                                                                 <  1:20705/141‑1 (MQ=255)
                                                                           ccGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTAc                                                         <  1:394738/141‑1 (MQ=255)
                                                                               ttCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGaa                                                     <  1:233758/141‑1 (MQ=255)
                                                                               ctCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGaa                                                     <  1:314418/140‑1 (MQ=255)
                                                                                   aaCGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATaca                                                 >  2:228148/1‑139 (MQ=255)
                                                                                       tcttctTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGa                                             <  1:298486/141‑1 (MQ=255)
                                                                                       tcttctTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGa                                             <  1:298480/141‑1 (MQ=255)
                                                                                             tAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAaataat                                       <  1:297363/141‑1 (MQ=255)
                                                                                                      tAATTGCATCTACTCTAAATCCATCACTGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAg                              <  1:45521/141‑1 (MQ=255)
                                                                                                         ttGCATCTACTCTAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCCCAGTaa                           >  2:97374/1‑140 (MQ=255)
                                                                                                                   cccaagccacacAAAGCCTATATCAAACCACCACTACATCATATCAAATACAGCATCCGTAACTTCCGGAGTACCCCAATTCAAATCAGCTTGTTTTTTACTGAACAAATGGAAATAAAATTGCTCAGTATTAGCATCata                 <  2:343615/130‑1 (MQ=255)
                                                                                                                    ctAAATCCATCAATGCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCatat                >  1:103732/1‑141 (MQ=255)
                                                                                                                            aTCACTGCCTATCTCACTCCCCCAGTTCCTCCTTTCACGTACTGAATTTCTTACTTACAAATTGGATCTAGTCAATTCATGTTGTTTTTTTCTGAATTAATGTAAATGATATTGCTCAGTATTAGCATCATATTCCCATGt        <  2:229666/141‑1 (MQ=255)
                                                                                                                                  gCCTATATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATcc  >  2:303412/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                        
TTTGTATACCTGGCTGATTCATATCTACATCAAATGCTGGGGCATATGTTTTACCCTCAGGTACAGGTAAGTCACCCGCTTCAAACGTCTTCTTAATATGCGTAATTGCATCTACTCTAAATCCATCAATGCCTTTATCAAACCACCAGTTCATCATTTCAAATACAGCATCTCTAACTTCCGGATTACCCCAATTCAAATCAGGTTGTTTTTTACTGAATAAATGGAAATAATATTGCTCAGTATTAGCATCATATTCCCATGTAGATCC  >  CP000730/1624539‑1624809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: