Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,830,883 C→T 76.1% K79K (AAG→AAA ezrA ← possible septation ring formation regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,830,8830CT76.1% 43.6 / 10.0 29K79K (AAG→AAAezrApossible septation ring formation regulator
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base T (11/11);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGAT  >  CP000730/1830759‑1831017
                                                                                                                            |                                                                                                                                      
aGCTTTGTTCGTAACTGTCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTa                                                                                                                        >  1:234804/1‑141 (MQ=255)
 gCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTCTCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCCCAGGAGCTAGATATTTATTTGTACTTTCtcc                                                                                                                       >  2:118534/1‑139 (MQ=255)
   tttGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGt                                                                                                                     >  2:274986/1‑141 (MQ=255)
     tGTTCGTAACTATACATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTg                                                                                                                   <  1:324198/141‑1 (MQ=255)
         cGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCt                                                                                                               >  1:18274/1‑141 (MQ=255)
            aaCTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCtttt                                                                                                            >  1:135463/1‑141 (MQ=255)
               tATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTGATGTGGACTTTCTACGTTGTCCTTTTTCa                                                                                                         <  1:191391/141‑1 (MQ=255)
                      cAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTTCGTTGTCCtttttttttgtttt                                                                                                  >  2:51487/1‑132 (MQ=255)
                         ccgcttttCATCATCAATTTCACATTCAGATGCGTTGAAACTAAAGTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGCTGCTAGATACTTATTTGTACCTTCTACGTTGTCCTTTTTCATTGCATCGTa                                                                                               <  2:29543/137‑1 (MQ=255)
                            aTTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTAttt                                                                                            >  1:333305/1‑141 (MQ=255)
                                gCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGtt                                                                                        >  2:23443/1‑141 (MQ=255)
                                         aTTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATTAAGCTTCAGCATTATGTATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCa                                                                               <  2:65901/141‑1 (MQ=255)
                                               cTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTACTGTTCCAAAGGAGGTAGAGATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCtttt                                                                         >  1:189776/1‑141 (MQ=255)
                                                tttttgaTGCTTTGAATCTAAATTTATTTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTa                                                                        <  1:113731/136‑1 (MQ=255)
                                                tttGAGATGCGTTGAAACTAAACTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTa                                                                        <  1:43913/141‑1 (MQ=255)
                                                           ttGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTGCACAGGAGCTAGATCTTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTTTCCTTTGAAGTTCAACTTa                                                             >  1:272638/1‑141 (MQ=255)
                                                             gAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGa                                                           <  1:180167/141‑1 (MQ=255)
                                                             gAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGa                                                           <  1:116661/141‑1 (MQ=255)
                                                                       ttATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCa                                                 <  2:307426/141‑1 (MQ=255)
                                                                              ataaAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGtttt                                          >  2:123196/1‑141 (MQ=255)
                                                                                  aGCCTCAGTATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATc                                      <  1:23443/141‑1 (MQ=255)
                                                                                     cTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCaaa                                   >  1:360174/1‑141 (MQ=255)
                                                                                      tCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCaaaa                                  >  2:56411/1‑141 (MQ=255)
                                                                                       cAGCATTATTGATTTTTTCGGCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAg                                 <  1:130302/141‑1 (MQ=255)
                                                                                          cATTATGGATTTTTTCTTCCACAGGAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTa                              >  1:256056/1‑141 (MQ=255)
                                                                                                                  gAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGtt      <  1:341104/141‑1 (MQ=255)
                                                                                                                  gAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGtt      <  1:351326/141‑1 (MQ=255)
                                                                                                                  gAGCTAGATATTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGtt      <  1:92144/141‑1 (MQ=255)
                                                                                                                      tAGATATTTATTTGTACTTTCTCCTTTGGCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGAt  >  1:291154/1‑141 (MQ=255)
                                                                                                                            |                                                                                                                                      
AGCTTTGTTCGTAACTATCCATCAACTCATTTGCATCATCAATTTCACTTTGAGATGCGTTGAAACTAAATTTATCTAATAAAGCCTCAGCATTATGGATTTTTTCTTCCACAGGAGCTAGATACTTATTTGTACTTTCTACGTTGTCCTTTTTCATTGCATCGTATTTCGTTTTTGTTTCACCTTTTAAATTCAACTTAGATAATTGTGCAAGGTTTTGATCAAAAGGTAACGTCTCAATTTCATTTTTACGTTCGAT  >  CP000730/1830759‑1831017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: