Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,004,062	(A)8→7	intergenic (‑649/+99)	rrs3 ← / ← perR	16S ribosomal RNA/Fur family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,004,055	0	A	.	100.0%	111.4 / NA	24	intergenic (‑642/+106)	rrs3/perR	16S ribosomal RNA/Fur family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (16/8);  total (16/8)

TCACTTTATGGATAGTTATTTAGTAGACCTTCGTTTGAATATTTTTATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCATAAAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAATTATTTATCTTGGCATTCTTTACAAACTCCATA  >  CP000730/2003914‑2004193                                                                                                                                              |                                                                                                                                           tCACTTTATGTATAGTTATTTAGTAGACCTTCGTTTGAATATTTTTATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCATaaaaaaa                                                                                                                                      <  2:347502/148‑1 (MQ=255)                 tATTTAGTAGACCTTCGTTTGAATATTTTTATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCAt                                                                                                                     >  1:108446/1‑148 (MQ=255)                 tATTTAGTAGACCTTCGTTTGAATATTTTTATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCAt                                                                                                                     >  1:238579/1‑148 (MQ=255)                  aTTTAGTAGACCTTCGTTTGAATATTTTTATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATg                                                                                                                    >  2:54575/1‑148 (MQ=255)                             cTTCGTTTGAATATTTTTATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAActt                                                                                                         >  1:22552/1‑148 (MQ=255)                                 gTTTGAATATTTTTATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTa                                                                                                     <  2:238525/148‑1 (MQ=255)                                      aaTATTTTTATGTTTACTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAatat                                                                                                <  2:16808/148‑1 (MQ=255)                                             ttATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGctt                                                                                         <  2:225379/148‑1 (MQ=255)                                             ttATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGctt                                                                                         <  2:190042/148‑1 (MQ=255)                                                    tatttatAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTtata                                                                                  >  1:69500/1‑148 (MQ=255)                                                                           taGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTa                                                           >  2:321946/1‑148 (MQ=255)                                                                            aGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTaa                                                          <  2:267480/148‑1 (MQ=255)                                                                               tAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAAttt                                                       >  2:260777/1‑148 (MQ=255)                                                                               tAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAAttt                                                       >  2:289974/1‑148 (MQ=255)                                                                               tAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAAttt                                                       >  1:132705/1‑148 (MQ=255)                                                                                   aCCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCa                                                   >  1:9634/1‑148 (MQ=255)                                                                                    ccACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCAt                                                  >  1:354165/1‑148 (MQ=255)                                                                                                         tcttttcttCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAAttattt                             <  1:387803/148‑1 (MQ=255)                                                                                                          cttttcttCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAAttatttt                            >  2:203281/1‑147 (MQ=255)                                                                                                          cttttcttCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTGTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAAttattta                            >  2:246810/1‑148 (MQ=255)                                                                                                           ttttcttCAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAAttatttat                           <  2:22552/148‑1 (MQ=255)                                                                                                                  cAATTTAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAATTATTTATCTTGGCa                    >  1:354386/1‑148 (MQ=255)                                                                                                                       tAAATATTTTTCAGAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAATTATTTATCTTGGCATTCtt               <  1:54575/148‑1 (MQ=255)                                                                                                                                    gAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAATTATTTATCTTGGCATTCTTTACAAACTCCATa  >  2:76021/1‑148 (MQ=255)                                                                                                                                    gAATAAGCAT‑AAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAATTATTTATCTTGGCATTCTTTACAAACTCCATa  >  2:84022/1‑148 (MQ=255)                                                                                                                                              |                                                                                                                                           TCACTTTATGGATAGTTATTTAGTAGACCTTCGTTTGAATATTTTTATGTTTATTTATAAAGGATACTAGTTATTAGTTAATACCACTTCCGTAGTTAGAATATTCTTTTCTTCAATTTAAATATTTTTCAGAATAAGCATAAAAAAAAGTTAAACAACTTTCATGTCGTTTAACTTCTTAAATATTACGCTTACTTATATGAAGTAAGCTAACGCTTTTTTAATTTGTCATACTACCAAAGTTAAATTATTTATCTTGGCATTCTTTACAAACTCCATA  >  CP000730/2003914‑2004193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑