| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 2,709,444 | (T)6→5 | coding (1483/1812 nt) | oatA ← | possible acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,709,439 | 0 | T | . | 91.9% | 142.9 / 5.8 | 37 | coding (1488/1812 nt) | oatA | possible acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base . (16/18); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACCTTTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATT > CP000730/2709300‑2709580 | ttattCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACCtttttagca < 2:110034/148‑5 (MQ=255)ttattCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACCtttttagca > 2:220306/1‑144 (MQ=255)ttattCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACCtttttagca > 1:227212/1‑144 (MQ=255)ttattCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACCtttttagca < 1:273353/148‑5 (MQ=255) aCCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCtt < 2:170479/148‑1 (MQ=255) aCCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCtt < 1:49735/148‑1 (MQ=255) tCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTtata > 1:403335/1‑148 (MQ=255) tCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTtata > 1:380316/1‑148 (MQ=255) tCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTtata > 2:345439/1‑148 (MQ=255) tCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTG‑TCTTTTTTAGCATAGTCTTtata > 2:1645/1‑148 (MQ=255) tCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGGCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCTAATTTGTACCAAACTCTACTACAACTTTTTGACC‑CTTTTAGCATAGTCTTtata > 2:368857/1‑148 (MQ=255) tAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTc < 2:31944/148‑1 (MQ=255) tAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTc < 2:125335/148‑1 (MQ=255) atatGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGt > 2:335318/1‑148 (MQ=255) tGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGc > 1:393280/1‑148 (MQ=255) tGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGATC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTc > 2:118912/1‑148 (MQ=255) tGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTc < 1:285000/148‑1 (MQ=255) tGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTc < 2:221784/148‑1 (MQ=255) ttttCCAAATCTATCCAATAGTTCATTTAATTTATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTGGTCC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCaa < 2:373206/148‑1 (MQ=255) cAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAAttt < 1:167179/148‑1 (MQ=255) aTAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTtgt < 2:412880/148‑1 (MQ=255) cATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCAttt > 1:202649/1‑148 (MQ=255) cATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTT‑TTCTTTTTTTTCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCAttt > 2:228576/1‑148 (MQ=255) tttAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTgg > 2:157233/1‑148 (MQ=255) gATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTc < 2:54826/148‑1 (MQ=255) cTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTa > 1:6223/1‑148 (MQ=255) cTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTa > 1:63191/1‑148 (MQ=255) cTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTa < 1:305444/148‑1 (MQ=255) ttCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGt < 2:408983/148‑1 (MQ=255) ttCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGCAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGt < 1:211919/148‑1 (MQ=255) ttCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATCTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGt < 1:25699/148‑1 (MQ=255) cGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTaa < 1:89913/148‑1 (MQ=255) tAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAg < 2:404420/148‑1 (MQ=255) aaaTGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGa > 2:201659/1‑148 (MQ=255) aaaTGCCCCATTTGTACCAAGCCCTACTACAACTTTTTGACC‑TCTTTAGCATAGTCTTTATATTGCGATGTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTCAGTAAAGa > 2:136974/1‑148 (MQ=255) gCCCCATTTGTACCAACCCCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCCTCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACAtt < 1:335318/148‑1 (MQ=255) aCCAAGCTCTACTACAACTTTTT‑TTTTTTTTTTTCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGTTATTTTCTTAGTAAAGACATTACCAATATcca > 2:236996/1‑148 (MQ=255) ccAAGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATccac > 1:302569/1‑148 (MQ=255) aGCTCTACTACAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACTTTACCAACATCCACCAt > 2:3086/1‑148 (MQ=255) acAACTTTTTGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTc < 1:180279/148‑1 (MQ=255) tttGACC‑TTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAAtt > 1:402263/1‑148 (MQ=255) | TTATTCTACCTTCATAATCTCTAGGTACTCTAATAGAAACTAAATATATGTCTGCTTTTCCAAAACTATCCAATAGTTCATTTAATTGATCTTTCGTAAATGCCCCATTTGTACCAAGCTCTACTACAACTTTTTGACCTTTTTTAGCATAGTCTTTATATTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATT > CP000730/2709300‑2709580 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |