Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	505,514	C→T	A469V (GCG→GTG)	treP →	PTS family trehalose (tre) porter component IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	505,514	0	C	T	100.0%	120.3 / NA	38	A469V (GCG→GTG)	treP	PTS family trehalose (tre) porter component IIBC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/21);  total (17/21)

GTTGGTGGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGCGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAATTT  >  CP000730/505384‑505645                                                                                                                                   |                                                                                                                                    gttggtgGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAg                                                                                                                    >  2:41326/1‑148 (MQ=255) gttggtgGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAg                                                                                                                    >  2:216943/1‑148 (MQ=255) gttggtgGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAg                                                                                                                    >  1:286073/1‑148 (MQ=255)    ggtggCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGAtt                                                                                                                 >  1:261338/1‑148 (MQ=255)      tggCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaa                                                                                                               <  1:72680/148‑1 (MQ=255)         cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  2:97144/148‑1 (MQ=255)         cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  1:151949/148‑1 (MQ=255)         cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  1:373108/148‑1 (MQ=255)         cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  2:43413/148‑1 (MQ=255)         cGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATtaataa                                                                                                            <  2:144172/148‑1 (MQ=255)                         tcaattcaaAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGcgt                                                                                            >  1:97088/1‑148 (MQ=255)                                  aaaGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTAttt                                                                                   <  1:174374/148‑1 (MQ=255)                                   aaGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTg                                                                                  <  1:327567/148‑1 (MQ=255)                                            ggCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCtt                                                                         >  2:341950/1‑148 (MQ=255)                                            ggCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCtt                                                                         <  2:251605/148‑1 (MQ=255)                                             gCCAGTATATGTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAAAAAAAAAGGGGCGTTCGTTATTTGGACGTCCttt                                                                        <  2:29954/148‑1 (MQ=255)                                                          aTTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAg                                                           <  2:28771/148‑1 (MQ=255)                                                                   gctattgctattGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAAtt                                                  <  1:284144/148‑1 (MQ=255)                                                                     tattgctattgTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATtgt                                                >  1:198204/1‑148 (MQ=255)                                                                        tgctattgTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATtgtgtg                                             <  2:261338/148‑1 (MQ=255)                                                                                ttgtACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCgaaaga                                     <  1:370301/148‑1 (MQ=255)                                                                                    aCCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATa                                 >  2:260335/1‑148 (MQ=255)                                                                                     ccATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAg                                >  2:317696/1‑148 (MQ=255)                                                                                              cTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGaa                       >  1:40769/1‑148 (MQ=255)                                                                                                     ttGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGt                <  2:316181/148‑1 (MQ=255)                                                                                                     ttGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGt                <  1:79811/148‑1 (MQ=255)                                                                                                     ttGTGATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGt                <  1:230280/148‑1 (MQ=255)                                                                                                       gtgATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTg              <  1:2833/148‑1 (MQ=255)                                                                                                       gtgATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTg              <  1:67463/148‑1 (MQ=255)                                                                                                         gATGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGta            >  2:162577/1‑148 (MQ=255)                                                                                                          aTGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGtat           >  1:233988/1‑148 (MQ=255)                                                                                                          aTGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGtat           >  2:192023/1‑148 (MQ=255)                                                                                                          aTGTCTCATTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGtat           >  2:41557/1‑148 (MQ=255)                                                                                                              ctcAGTTTAGGAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATACGTTCTAAATCa       >  2:327931/1‑148 (MQ=255)                                                                                                                 aTTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATCAAATAAGAAAGGGGCGTTCGTTATTTGGACGCCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAAt    >  1:357729/1‑148 (MQ=255)                                                                                                                 aTTTTAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAAt    >  1:236995/1‑148 (MQ=255)                                                                                                                   tttAGTAAACAAAAAGTGAAAGAAATTGTTGAATATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAATAAATAGATTGGAGAAAATCCGTTGTATATCAAAttt  <  2:229736/148‑1 (MQ=255)                                                                                                                   tttAGTAAACAAAAAGTGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAAttt  <  1:391536/148‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    GTTGGTGGCGTGCCAGCATTCATTTCAATTCAAAAAGAATTTTGGCCAGTATATCTTATTGTGACAGCTATTGCTATTGTTGTACCATGTATACTAACAATTGTGATGTCTCATTTTAGTAAACAAAAAGCGAAAGAAATTGTTGAAGATTAATAAAATAAAAAAGGGGCGTTCGTTATTTGGACGTCCTTTATTACGTTATAAGGTGGTAATTGTGTGTCGAAAGAAATAGATTGGAGAAAATCCGTTGTATATCAAATTT  >  CP000730/505384‑505645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑