Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	744,375	A→T	intergenic (+2212/‑714)	yetJ → / → sarX	hypothetical membrane protein/possible staphylococcal accessory protein X

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	744,375	0	A	T	100.0%	133.0 / NA	41	intergenic (+2212/‑714)	yetJ/sarX	hypothetical membrane protein/possible staphylococcal accessory protein X
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/25);  total (16/25)

AATAAAGCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATATTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAA  >  CP000730/744233‑744516                                                                                                                                               |                                                                                                                                              aaTAAAGCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGaa                                                                                                                                          <  1:14269/148‑1 (MQ=255)       gCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGaa                                                                                                                                    >  1:104416/1‑148 (MQ=255)       gCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGaa                                                                                                                                    >  1:304577/1‑148 (MQ=255)        cGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGaaa                                                                                                                                   <  2:142390/148‑1 (MQ=255)        cGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGaaa                                                                                                                                   <  2:140497/148‑1 (MQ=255)            ttAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCt                                                                                                                               <  1:161419/148‑1 (MQ=255)            ttAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCt                                                                                                                               <  1:66073/148‑1 (MQ=255)                    gattcgTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTc                                                                                                                       <  1:19445/148‑1 (MQ=255)                           taataaTAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGt                                                                                                                >  1:359652/1‑148 (MQ=255)                            aataatAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAATCAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGtt                                                                                                               <  1:248433/148‑1 (MQ=255)                                  aGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAAt                                                                                                         <  2:80445/148‑1 (MQ=255)                                  aGATAATGCAAATTTAAGTAGACATTCTTTGGAAAGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAAt                                                                                                         >  2:415789/1‑148 (MQ=255)                                    aTAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCa                                                                                                       >  2:340152/1‑148 (MQ=255)                                     tAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCAt                                                                                                      >  1:250023/1‑148 (MQ=255)                                      aaTGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATa                                                                                                     <  2:385196/148‑1 (MQ=255)                                          cAAATTTAAGTAGCCCTTATTTGATGGGGTAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACt                                                                                                 <  2:217985/148‑1 (MQ=255)                                           aaaTTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTg                                                                                                <  1:327056/148‑1 (MQ=255)                                             aTTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCt                                                                                              <  2:51690/148‑1 (MQ=255)                                             aTTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCt                                                                                              <  2:380436/148‑1 (MQ=255)                                                 aaGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTtaat                                                                                          <  2:107761/148‑1 (MQ=255)                                                   gTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTtaataa                                                                                        <  1:59877/148‑1 (MQ=255)                                                           tATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAAc                                                                                >  2:63754/1‑148 (MQ=255)                                                           tATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAAc                                                                                >  1:338150/1‑148 (MQ=255)                                                                                  gTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTACATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCa                                                         <  2:160304/148‑1 (MQ=255)                                                                                   ttACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCAt                                                        <  1:116413/148‑1 (MQ=255)                                                                                   ttACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCAt                                                        <  1:287794/148‑1 (MQ=255)                                                                                             aaaaTATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATaa                                              >  2:321959/1‑148 (MQ=255)                                                                                              aaaTATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAg                                             <  2:315656/148‑1 (MQ=255)                                                                                                atatTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGAc                                           >  1:16460/1‑148 (MQ=255)                                                                                                atatTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGAc                                           >  1:303323/1‑148 (MQ=255)                                                                                                        cATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTGAATAATAAAGAACGAGATCTAGGTAATAGTGCTCCATTAATTTATAAGACATTAAGTg                                   >  1:144768/1‑148 (MQ=255)                                                                                                          ttttAAAAATTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTgcg                                 >  1:389442/1‑148 (MQ=255)                                                                                                               aaaaTTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTa                            >  1:259525/1‑148 (MQ=255)                                                                                                                 aaTTTAAAACAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATc                          <  1:415789/148‑1 (MQ=255)                                                                                                                      aaaaCAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAAc                     <  2:105026/148‑1 (MQ=255)                                                                                                                      aaaaCAAGTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAAc                     <  1:332514/148‑1 (MQ=255)                                                                                                                             gTAATTAATGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATg              <  1:2379/148‑1 (MQ=255)                                                                                                                                attaatGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCt           <  1:370191/148‑1 (MQ=255)                                                                                                                                    atGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTgg       <  2:238259/148‑1 (MQ=255)                                                                                                                                     tGAATTGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGa      >  2:353012/1‑148 (MQ=255)                                                                                                                                         ttGGATTTTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTaa  >  1:137787/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                              AATAAAGCGACTTAAATTCGATTCGTTAATAATAGATAATGCAAATTTAAGTAGCCCTTATTTGATGGGGGAAAGTGATGAGTTACTATTGAAAAATATTTTGCATTTTAAAAATTTAAAACAAGTAATTAATGAATTGGATATTGAACAAGAAAAGCTGATTTTTCTAAATGTTGAAAATCATAAACTGCTTAATAATAAAGAACGAGATCTAAGTAATAGTGCTCCATTAATTTATAAGACATTAAGTGCGCTGTATCACAACTTTGATGGCTTTGGATTAA  >  CP000730/744233‑744516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑