Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	749,249	C→T	S59S (AGC→AGT)	USA300HOU_0696 →	MFS family major facilitator transporter, sugar:cation symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	749,249	0	C	T	100.0%	114.1 / NA	36	S59S (AGC→AGT)	USA300HOU_0696	MFS family major facilitator transporter, sugar:cation symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/20);  total (16/20)

ATTATAAACTCTTTGTTGCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGCCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATCTGGTTATTCATATT  >  CP000730/749107‑749392                                                                                                                                               |                                                                                                                                                aTTATAAACTCTTTGTTGCTAATATGTTGCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGtt                                                                                                                                            <  2:340637/148‑1 (MQ=255)  ttATAAACTCTTTGTTGCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTTTTTTTTTTTATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGttt                                                                                                                                           >  2:412542/1‑148 (MQ=255)  ttATAAACTCTTTGTTGCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGttt                                                                                                                                           <  1:112790/148‑1 (MQ=255)  ttATAAACTCTTTGTTGCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGttt                                                                                                                                           <  1:139993/148‑1 (MQ=255)    ataAACTCGTGGTTGCTAATATGTTTCTACGAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACGAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTAc                                                                                                                                         <  2:233387/148‑1 (MQ=255)           ctTTGTTGCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAAt                                                                                                                                  >  1:164586/1‑148 (MQ=255)           ctTTGTTGCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAAt                                                                                                                                  >  2:14778/1‑148 (MQ=255)           ctTTGTTGCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAAt                                                                                                                                  >  2:108554/1‑148 (MQ=255)             ttgttgCTAATATGTTTCTACTAGGTCTGGGTATTGCCGTTACGGTCCCACATCTTGTTCATTTTGCCCCTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTc                                                                                                                                <  1:104124/148‑1 (MQ=255)                 tgCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAatt                                                                                                                            <  1:57550/148‑1 (MQ=255)                         tGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGTATTTTAATTTTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTCCAGTAAATTCAATTATTGCTAg                                                                                                                    >  2:138288/1‑148 (MQ=255)                         tGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAg                                                                                                                    >  2:39248/1‑148 (MQ=255)                                       aTGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGc                                                                                                      <  1:24145/148‑1 (MQ=255)                                       aTGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGc                                                                                                      >  1:328461/1‑148 (MQ=255)                                       aTGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGc                                                                                                      <  1:332829/148‑1 (MQ=255)                                       aTGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGc                                                                                                      <  1:24139/148‑1 (MQ=255)                                       aTGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATCACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGc                                                                                                      >  1:53903/1‑148 (MQ=255)                                                            ccATATCTTGTTCTTTTTGCAACTAAAGATCTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAatta                                                                                 <  1:173252/148‑1 (MQ=255)                                                                    tGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAattattattatt                                                                         <  2:147028/148‑1 (MQ=255)                                                                     gTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATtc                                                                        >  2:398832/1‑148 (MQ=255)                                                                      ttCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATtct                                                                       >  1:276069/1‑148 (MQ=255)                                                                      ttCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATtct                                                                       >  1:278130/1‑148 (MQ=255)                                                                                 aCTAAAGATTTAGGTAGGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTaa                                                            >  1:365959/1‑148 (MQ=255)                                                                                  cTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAAt                                                           <  1:167294/148‑1 (MQ=255)                                                                                                  gACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGtttt                                           <  1:245706/148‑1 (MQ=255)                                                                                                  gACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGtttt                                           <  2:353640/148‑1 (MQ=255)                                                                                                             aGTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGCTTTTCGGATACGCATCACTTTAATAGAAATATTATTATTATTCTCGCATTATTAGTGGGTGCGCTTGGTTTTTCAATATACtt                                <  2:194979/148‑1 (MQ=255)                                                                                                              gTATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACttt                               >  1:370671/1‑148 (MQ=255)                                                                                                               tATGGATTACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACtttt                              <  1:412542/148‑1 (MQ=255)                                                                                                                      tACTTCTAGCATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGa                       >  1:374764/1‑148 (MQ=255)                                                                                                                               cATCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATCTg              <  1:414175/148‑1 (MQ=255)                                                                                                                                aTCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATCTgg             >  1:128670/1‑148 (MQ=255)                                                                                                                                 tCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATCTGGt            <  1:398832/148‑1 (MQ=255)                                                                                                                                 tCTGCAGCGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATCTGGt            >  1:249253/1‑148 (MQ=255)                                                                                                                                        cGATTAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATCTGGTTATTCat     <  2:32812/148‑1 (MQ=255)                                                                                                                                           ttAGTCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATCTGGTTATTCATAtt  <  2:325470/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                ATTATAAACTCTTTGTTGCTAATATGTTTCTACTAGGTATGGGTATTGCGGTTACGGTCCCATATCTTGTTCTTTTTGCAACTAAAGATTTAGGTATGACAACAAATCAGTATGGATTACTTCTAGCATCTGCAGCGATTAGCCAGTTTACAGTAAATTCAATTATTGCTAGATTTTCGGATACGCATCACTTTAATAGAAAAATTATTATTATTCTCGCATTATTAATGGGTGCGCTTGGTTTTTCAATATACTTTTTTGTAGATACAATCTGGTTATTCATATT  >  CP000730/749107‑749392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑