Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	39,195	T→C	K647E (AAA→GAA)	mecA ←	penicillin binding protein 2 prime

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	39,195	0	T	C	100.0%	153.4 / NA	42	K647E (AAA→GAA)	mecA	penicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/15);  total (27/15)

GAAGTAACAGCACTTATTAATAATTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCA  >  CP000730/39052‑39334                                                                                                                                                |                                                                                                                                            gaAGTAACAGCACTTATTAATAATTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTAGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCCCATCATACACTTTACCTGAGATTTCGGCa                                                                                                                                         >  2:81876/1‑148 (MQ=255)  aaGTAACAGCACTTATTAATAATTCATAATAAAACAGTGAAGCAACCATCGTTCAGGATTACGTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCAt                                                                                                                                        <  1:235699/148‑1 (MQ=255)  aaGTAACAGCACTTATTAATAATTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCAt                                                                                                                                        <  1:42256/148‑1 (MQ=255)         aGCACTTATTAATAATTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGtagct                                                                                                                                 >  1:64103/1‑148 (MQ=255)         aGCACTTATTAATAATTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGtagct                                                                                                                                 >  2:83508/1‑148 (MQ=255)          gCACTTATTAATAATTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACGCTTTACCTGAGATTTCGGCATTGtagcta                                                                                                                                >  1:411608/1‑148 (MQ=255)                attaACAATTCATAATAAAACAGTGAAGCAACCATCGTTACTGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCAtt                                                                                                                          <  2:264079/148‑1 (MQ=255)                  taataaTTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTcc                                                                                                                        <  1:163782/148‑1 (MQ=255)                  taataaTTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTcc                                                                                                                        <  2:129934/148‑1 (MQ=255)                   aataatTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACGCTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCt                                                                                                                       <  2:345726/148‑1 (MQ=255)                               taaAACAGTGAAGCAACCATCATTACGGATTGCTTCACTTTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACa                                                                                                           <  2:244751/148‑1 (MQ=255)                                 aaaCAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATc                                                                                                         >  2:160402/1‑148 (MQ=255)                                   aCAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCtt                                                                                                       >  1:96067/1‑148 (MQ=255)                                    cAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCttt                                                                                                      <  1:330301/148‑1 (MQ=255)                                     aGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTa                                                                                                     <  2:406811/148‑1 (MQ=255)                                     aGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTa                                                                                                     <  2:143311/148‑1 (MQ=255)                                                            ttGTTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAACCATCATCATGtt                                                                              >  2:291446/1‑148 (MQ=255)                                                                 tCACTGTNTTGTTATTCCTCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGAt                                                                         <  2:312492/148‑1 (MQ=255)                                                                   aCTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTa                                                                       <  1:164316/148‑1 (MQ=255)                                                                            ttATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCa                                                              <  2:105844/148‑1 (MQ=255)                                                                               ttCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCatat                                                           <  2:232965/148‑1 (MQ=255)                                                                                    cTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGatat                                                      >  1:69414/1‑148 (MQ=255)                                                                                                ttttATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAAt                                          >  1:401682/1‑148 (MQ=255)                                                                                                ttttATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAAt                                          >  1:86428/1‑148 (MQ=255)                                                                                                ttttATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAAt                                          >  1:371571/1‑148 (MQ=255)                                                                                                ttttATTACCGTTCTCATATAGCACATCATACACTTTACTTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACNACCCAAt                                          >  2:312976/1‑148 (MQ=255)                                                                                                        ccGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGc                                  >  2:135707/1‑148 (MQ=255)                                                                                                        ccGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGc                                  >  1:288359/1‑148 (MQ=255)                                                                                                        ccGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGc                                  >  1:38942/1‑148 (MQ=255)                                                                                                        ccGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATGAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGc                                  >  2:350555/1‑148 (MQ=255)                                                                                                            tctcATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCGAATTTGTCTGCCAGt                              >  1:344842/1‑148 (MQ=255)                                                                                                            tctcATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGt                              >  1:25282/1‑148 (MQ=255)                                                                                                            tctcATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGt                              >  1:214211/1‑148 (MQ=255)                                                                                                             ctcATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGtt                             >  1:200885/1‑148 (MQ=255)                                                                                                             ctcATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGtt                             >  1:73032/1‑148 (MQ=255)                                                                                                             ctcATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGtt                             >  1:260239/1‑148 (MQ=255)                                                                                                             ctcATATAGCTCATCATACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGtt                             >  1:72963/1‑148 (MQ=255)                                                                                                                           catACACTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCAt               <  1:83508/148‑1 (MQ=255)                                                                                                                              acacTTTACCTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCAtttt            >  1:281194/1‑148 (MQ=255)                                                                                                                                      ccTGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCTTTGTTTCATTTTGAGTTCTg    >  1:224752/1‑148 (MQ=255)                                                                                                                                        tGAGATTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCa  >  2:52131/1‑148 (MQ=255)                                                                                                                                        cgagaTTTCGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCa  >  2:225730/2‑148 (MQ=255)                                                                                                                                                |                                                                                                                                            GAAGTAACAGCACTTATTAATAATTCATAATAAAACAGTGAAGCAACCATCGTTACGGATTGCTTCACTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCA  >  CP000730/39052‑39334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑