Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	890,260	T→C	V39A (GTT→GCT)	gcvH →	glycine cleavage system protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	890,260	0	T	C	100.0%	134.3 / NA	36	V39A (GTT→GCT)	gcvH	glycine cleavage system protein H
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/20);  total (16/20)

TAATTAGGAGGGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGTTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAACGAAGAACTAGAAGATAGTC  >  CP000730/890126‑890403                                                                                                                                       |                                                                                                                                                taattaGGAGGGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTa                                                                                                                                    >  2:283071/1‑148 (MQ=255) taattaGGAGGGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTa                                                                                                                                    <  1:28120/148‑1 (MQ=255) taattaGGAGGGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTa                                                                                                                                    <  1:28123/148‑1 (MQ=255) taattaGGAGGGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTa                                                                                                                                    <  1:29889/148‑1 (MQ=255)  aattaGGAGGGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTAc                                                                                                                                   <  1:158398/148‑1 (MQ=255)      aggaggGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGa                                                                                                                               >  2:49075/1‑148 (MQ=255)         aggGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAAc                                                                                                                            >  1:27082/1‑148 (MQ=255)         aggGGATTCTCTTGGCAGTACCAAATGAATCGAAATATTCAAAAGAGCATGACTGGGTAAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAAc                                                                                                                            >  2:154644/1‑148 (MQ=255)             gATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTACAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAgat                                                                                                                        <  1:235723/148‑1 (MQ=255)                     tGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAAt                                                                                                                <  1:252825/148‑1 (MQ=255)                                           atatTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGt                                                                                          <  2:133506/148‑1 (MQ=255)                                               tCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCg                                                                                      >  1:118605/1‑148 (MQ=255)                                                                    aaaGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTAt                                                                 >  1:336676/1‑148 (MQ=255)                                                                                       cAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAAt                                              <  1:362733/148‑1 (MQ=255)                                                                                       cAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAAt                                              <  1:336697/148‑1 (MQ=255)                                                                                         ataatTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAAtct                                            <  2:6080/148‑1 (MQ=255)                                                                                         ataatTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAAtct                                            <  1:325112/148‑1 (MQ=255)                                                                                         ataatTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAAtct                                            <  2:367253/148‑1 (MQ=255)                                                                                         ataatTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAAtct                                            <  2:2261/148‑1 (MQ=255)                                                                                           aatTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATctct                                          <  1:24141/148‑1 (MQ=255)                                                                                               ggAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTa                                      >  2:206359/1‑148 (MQ=255)                                                                                                 aaTCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTaaa                                    >  1:70798/1‑148 (MQ=255)                                                                                                 aaTCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTaaa                                    >  1:229584/1‑148 (MQ=255)                                                                                                 aaTCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTaaa                                    >  1:168270/1‑148 (MQ=255)                                                                                                 aaTCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTaaa                                    >  2:337801/1‑148 (MQ=255)                                                                                                 aaTCACAGAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTaaa                                    >  1:69267/1‑148 (MQ=255)                                                                                                        gAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTg                             <  2:317312/148‑1 (MQ=255)                                                                                                        gAATACGCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTg                             <  2:175229/148‑1 (MQ=255)                                                                                                              gCACAAAGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCa                       <  2:108271/148‑1 (MQ=255)                                                                                                                  aaaGCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAACga                   >  1:43146/1‑148 (MQ=255)                                                                                                                     gCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAACgaaga                <  2:140416/148‑1 (MQ=255)                                                                                                                     gCGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAACgaaga                <  1:49075/148‑1 (MQ=255)                                                                                                                      cGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAACgaagaa               >  2:119327/1‑148 (MQ=255)                                                                                                                      cGAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAACgaagaa               >  2:119333/1‑148 (MQ=255)                                                                                                                       gAGTTAGGTGATATTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAACGAAGAAc              <  1:283071/148‑1 (MQ=255)                                                                                                                                   atTGCTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTAACAGAAGAACTAGAAGATAGTc  >  1:3408/1‑148 (MQ=255)                                                                                                                                       |                                                                                                                                                TAATTAGGAGGGGATTCTCTTGGCAGTACCAAATGAATTGAAATATTCAAAAGAGCATGAATGGGTTAAAGTTGAAGGTAATGTAGCAATAATTGGAATCACAGAATACGCACAAAGCGAGTTAGGTGATATTGTTTTCGTTGAATTACCAGAAACAGATGATGAAATTAATGAAGGGGATACGTTTGGTAGCGTAGAATCAGTTAAAACTGTATCAGAATTATATGCACCAATCTCTGGTAAAGTAGTTGAAGTCAACGAAGAACTAGAAGATAGTC  >  CP000730/890126‑890403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑