Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,298,108	T→A	intergenic (+4/‑232)	pnp → / → rnjB	polyribonucleotide nucleotidyltransferase/beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,298,108	0	T	A	100.0%	61.5 / NA	20	intergenic (+4/‑232)	pnp/rnjB	polyribonucleotide nucleotidyltransferase/beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (10/10);  total (10/10)

ACATTTCACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCTGTAT  >  CP000730/1297968‑1298245                                                                                                                                             |                                                                                                                                          acaTTTCACAAATTTCAAAAAATATAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTc                                                                                                                                    <  1:111215/148‑1 (MQ=255)   aTTTCACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCat                                                                                                                                  <  1:69343/148‑1 (MQ=255)         caAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATgacg                                                                                                                            >  2:276827/1‑148 (MQ=255)                                         aGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCa                                                                                            >  2:387384/1‑148 (MQ=255)                                                       tCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTg                                                                              >  1:131195/1‑148 (MQ=255)                                                       tCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTg                                                                              >  1:131200/1‑148 (MQ=255)                                                                                              aaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATATTAGATAACGTCATATGATGACAATGGATCGTCATGTGATTTTTTTATGCCACTTCTTACGATGTGACCCGTTTTGAATTTGTTGTATTGTACATTTTaatccgc                                       <  2:97796/148‑6 (MQ=255)                                                                                              aaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGc                                       <  1:130921/148‑1 (MQ=255)                                                                                              aaCAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGc                                       <  1:259472/148‑1 (MQ=255)                                                                                                 aaGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCttt                                    >  2:404153/1‑148 (MQ=255)                                                                                                     tCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTttatta                                <  1:412983/148‑1 (MQ=255)                                                                                                                cTTCACATAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTTTGTTTTTTTTTACGAAGTGACCCGTTTTGTATTTGTTGTATTGAACATTTTAAAACGCTTTCTTATTTTGTGTGCa                     >  2:237226/1‑148 (MQ=255)                                                                                                                   cacaTAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACt                  >  1:79338/1‑148 (MQ=255)                                                                                                                   cacaTAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACt                  >  1:328181/1‑148 (MQ=255)                                                                                                                   cacaTAGAGCATTAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACt                  >  1:72437/1‑148 (MQ=255)                                                                                                                              ttAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATcc       <  1:227685/148‑1 (MQ=255)                                                                                                                              ttAGAAGAATAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATcc       <  1:404153/148‑1 (MQ=255)                                                                                                                                agaagaATAATATATAAAGTCAGATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTTAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCtg     <  2:247245/148‑1 (MQ=255)                                                                                                                                 gaagaaTAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCtgt    <  1:276827/148‑1 (MQ=255)                                                                                                                                   agaaTAATATATAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTTACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTTTGTGCAACTGTTAATTATCCtgtat  >  2:240820/1‑148 (MQ=255)                                                                                                                                             |                                                                                                                                          ACATTTCACAAATTTCAAAAAATAGAATTGAAAAAGTGGAAGATGTATTAAAAATCGGTGACACAATTGAAGTTAAGATTACTGAAATTGATAAACAAGGTCGAGTAAATGCTTCACATAGAGCATTAGAAGAATAATATTTAAAGTCATATGACGACAATGTATCGTCATGTGATTTTTTTATGCCACTTTTTACGAAGTGACCCGTTTTGAATTTGTTGTATTGAACATTTTAAAACGCTTTATTATTTTGTGTGCAACTGTTAATTATCCTGTAT  >  CP000730/1297968‑1298245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑