Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,851,260 C→A A115S (GCG→TCG)  acsA1 ← acetate‑‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,851,2600CA96.5% 90.1 / ‑2.2 29coding (343/1707 nt)acsA1acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (12/16);  minor base . (1/0);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CTACAGGTACTCGAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGCAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCTTTTCTGTGC  >  CP000730/1851116‑1851399
                                                                                                                                                |                                                                                                                                           
cTACAGGTACTCGAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGaaaa                                                                                                                                          <  2:139094/148‑1 (MQ=255)
cTACAGGTAATCGAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGaaaa                                                                                                                                          <  2:82892/148‑1 (MQ=255)
    aGGTACTCGAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAatat                                                                                                                                      >  1:23854/1‑148 (MQ=255)
                           tattaGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaa                                                                                                               <  1:52965/148‑1 (MQ=255)
                           tattaGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaa                                                                                                               <  1:231332/148‑1 (MQ=255)
                            attaGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaat                                                                                                              <  1:387005/148‑1 (MQ=255)
                            attaGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaat                                                                                                              <  1:184700/148‑1 (MQ=255)
                               aGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACataaatata                                                                                                           >  1:25265/1‑148 (MQ=255)
                                                           ttataaagatatgCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTg                                                                               >  2:196869/13‑148 (MQ=255)
                                                             tAATCTATCCGCAACTGCCTTTTCCATAAATGTTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCa                                                                             >  2:398463/1‑148 (MQ=255)
                                                             tAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCa                                                                             >  2:414071/1‑148 (MQ=255)
                                                             tAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCa                                                                             >  2:336839/1‑148 (MQ=255)
                                                                tCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGt                                                                          >  1:402702/1‑148 (MQ=255)
                                                                 cTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTc                                                                        >  1:277982/1‑148 (MQ=255)
                                                                               cTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTc                                                           >  1:353852/1‑148 (MQ=255)
                                                                                    ccATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGTCATAATTATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCgctgc                                                      <  1:95628/148‑1 (MQ=255)
                                                                                    ccATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCgctgc                                                      <  1:58300/148‑1 (MQ=255)
                                                                                        aaaTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTtta                                                  >  1:281589/1‑148 (MQ=255)
                                                                                        aaaTGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTtta                                                  >  1:340304/1‑148 (MQ=255)
                                                                                           tGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTAAGGTGTACTCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATTTTCAGACAAAACATTCGCTGCTttattt                                               >  2:290412/1‑147 (MQ=255)
                                                                                                    tAACGTCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGt                                      <  2:162127/148‑1 (MQ=255)
                                                                                                    tAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCTACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGt                                      >  2:314261/1‑148 (MQ=255)
                                                                                                     aaCGGCCCAACAATTTCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGtt                                     <  2:165937/148‑1 (MQ=255)
                                                                                                                   tGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATaa                       <  1:196869/148‑1 (MQ=255)
                                                                                                                   tGCACCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATaa                       <  1:414021/148‑1 (MQ=255)
                                                                                                                      aCCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGta                    <  1:48167/148‑1 (MQ=255)
                                                                                                                      aCCAATTTTTAAAACACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGta                    <  1:43054/148‑1 (MQ=255)
                                                                                                                                  aaCACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCtttt        <  2:297631/148‑1 (MQ=255)
                                                                                                                                  aaCACCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCtttt        <  1:30078/148‑1 (MQ=255)
                                                                                                                                    cacCTAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCTTTTCt      <  1:130551/148‑1 (MQ=255)
                                                                                                                                        tAACAACGAAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCTTTTCTGTGc  <  2:402702/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                           
CTACAGGTACTCGAGGTAACAATGCCTTATTAGTAATTAACACTTTAGCTTCACTGTTCTCTAATCTATCCGCAACTGCCTTTTCCATAAATGCTTCAAATAACGGCCCAACAATTGCACCAATTTTTAAAACACCTAACAACGCAAAATATAGTTCAGGTGTACGCGACATAAATATAAATACTCTGTCACCTTTGTCAACTTCTGCATGTTCAGACAAAACATTCGCTGCTTTATTAGATAACCGTTGCATATCTTTATAAGTATACGATTCTTTTCTGTGC  >  CP000730/1851116‑1851399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: