Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,079,114	T→C	H201R (CAC→CGC)	bcrA ←	ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,079,114	0	T	C	100.0%	77.3 / NA	23	H201R (CAC→CGC)	bcrA	ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

AAATCCCGTACGCTTATGTTCTTCATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGTGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAGTAGGCTC  >  CP000730/2078972‑2079256                                                                                                                                               |                                                                                                                                               aaaTCCCGTACGCTTATGTTCTTCATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATg                                                                                                                                           >  1:71099/1‑148 (MQ=255)              ttATGTTCTTCATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTa                                                                                                                              >  2:381433/1‑148 (MQ=255)                    tcttcATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTAtttttt                                                                                                                        >  2:182866/1‑148 (MQ=255)                                  tAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATa                                                                                                          >  2:259695/1‑148 (MQ=255)                                              ttCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGAc                                                                                              >  1:260007/1‑148 (MQ=255)                                                                  tAGCCCCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTATATTTtc                                                                          <  1:395163/148‑1 (MQ=255)                                                                  tAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTTAGATAATGTGTGTCGACATGAATATTGTTTTATTTtc                                                                          <  2:374043/148‑1 (MQ=255)                                                                  tAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTtc                                                                          <  1:209210/148‑1 (MQ=255)                                                                       ccacTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTATATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCaa                                                                     >  2:265175/1‑148 (MQ=255)                                                                                aCTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCtt                                                            <  1:182866/148‑1 (MQ=255)                                                                                 cTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTg                                                           >  2:353922/1‑148 (MQ=255)                                                                                                 cAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAAt                                           <  1:49537/148‑1 (MQ=255)                                                                                                   gttgttCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAg                                         <  2:222248/148‑1 (MQ=255)                                                                                                   gttgttCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAg                                         <  2:395140/148‑1 (MQ=255)                                                                                                   gttgttCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAg                                         <  2:26103/148‑1 (MQ=255)                                                                                                                 ccATTTAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTTTTTTTCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTAATCAATTAATTATTGCTGAATTATATCCAATAGCTCATTTTTAGcaa                           >  2:15863/1‑146 (MQ=255)                                                                                                                      tAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGa                      <  1:149478/148‑1 (MQ=255)                                                                                                                      tAAGATAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGa                      <  2:236498/148‑1 (MQ=255)                                                                                                                          aTAACTTCACCGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCaa                  >  1:179217/1‑148 (MQ=255)                                                                                                                                   ccGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAg         <  2:398263/148‑1 (MQ=255)                                                                                                                                   ccGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAg         <  2:391294/148‑1 (MQ=255)                                                                                                                                   ccGTCACTTAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAg         <  2:11379/148‑1 (MQ=255)                                                                                                                                          ttAAGCGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAGTAGGCtc  <  1:122179/148‑1 (MQ=255)                                                                                                                                               |                                                                                                                                               AAATCCCGTACGCTTATGTTCTTCATAAATAAGTAGAGATGCTAATTCATCATCAAGGTCCTCAATAGCACCACTCACAACTTGATATCTTTGTAACAGTTGTTCTTTTGAACCATTTAAGATAACTTCACCGTCACTTAAGTGAATGATATAATCAGCTATTTTTTCTAAATCTGAGATAATGTGTGTCGACATGAATATTGTTTTATTTTCATCAATTAATTCTTGCTGAATTATCTCCAATAGCTCATTTCTAGCTAAGGGATCAAGACCTGAAGTAGGCTC  >  CP000730/2078972‑2079256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑