Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,272,733	C→T	intergenic (‑321/‑342)	glmS ← / → mtlF	glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)/PTS family fructose/mannitol (fru) porter component IIBC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,272,733	0	C	T	100.0%	80.7 / NA	25	intergenic (‑321/‑342)	glmS/mtlF	glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)/PTS family fructose/mannitol (fru) porter component IIBC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/13);  total (12/13)

TCTTATTAACTTTGTCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGCACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTACTGCCGAACTTTCCCCCTTTCCATTAATCATTATTGTACAACGGTGTTGTT  >  CP000730/2272605‑2272861                                                                                                                                 |                                                                                                                                 tCTTATTAACTTTGTCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAGTACATATATTGTACTCTATAAAGGCCACTCa                                                                                                               >  2:4739/1‑148 (MQ=255) tCTTATTAACTTTGTCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCa                                                                                                               <  1:336023/148‑1 (MQ=255) tCTTATTAACTTTGTCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCa                                                                                                               <  1:178669/148‑1 (MQ=255) tCTTATTAACTTTGTCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCa                                                                                                               >  2:362365/1‑148 (MQ=255) tCTTATTAACTTTCTCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCa                                                                                                               <  1:90782/148‑1 (MQ=255)          cTTTGTCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAAc                                                                                                      >  1:88455/1‑148 (MQ=255)               tCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTa                                                                                                 >  2:195301/1‑148 (MQ=255)                     aaGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTc                                                                                           <  1:77869/148‑1 (MQ=255)                        tCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTcaaa                                                                                        <  1:129707/148‑1 (MQ=255)                           cccTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTcaaatac                                                                                     >  1:292592/1‑148 (MQ=255)                                   ttttACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAAtattt                                                                             <  1:228609/148‑1 (MQ=255)                                   ttttACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAAtattt                                                                             <  2:259242/148‑1 (MQ=255)                                   ttgtACTTAATAAGCTAGCGAATGGGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTCTCCTCCTCGTCTACAAATTCATATTTTGTACTCTATAAAGGCCACTCCTATATTAACCTTTAATCTTCAAATACAAAtattt                                                                             <  2:386187/145‑1 (MQ=255)                                    tttACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAAtattta                                                                            >  1:43820/1‑148 (MQ=255)                                              aaGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAgg                                                                  <  1:263879/148‑1 (MQ=255)                                                gCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGgcg                                                                >  2:64785/1‑148 (MQ=255)                                                        aTGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACt                                                        <  1:10366/148‑1 (MQ=255)                                                        aTGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACt                                                        >  1:294393/1‑148 (MQ=255)                                                            gCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTAc                                                    <  2:52940/148‑1 (MQ=255)                                                               acaTCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTACTGc                                                 >  2:340426/1‑148 (MQ=255)                                                               acaTCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTACTGc                                                 >  1:163891/1‑148 (MQ=255)                                                                               ccgccgATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTACTGCCGAACTTTCCCCCttt                                 <  2:65887/148‑1 (MQ=255)                                                                                         aTTCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTACTGCCGAACTTTCCCCCTTTCCATTAATCa                       <  1:362365/148‑1 (MQ=255)                                                                                           tCGATCACTATCCTCCTCGTCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTACTGCCGAACTTTCCCCCTTTCCATCAATCatt                     >  1:190154/1‑148 (MQ=255)                                                                                                              tCTACAAATACATATATTGTACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTACTGCCGAACTTTCCCCCTTTCCATTAATCATTATTGTACAACGGTgttgtt  >  2:280231/1‑148 (MQ=255)                                                                                                                                 |                                                                                                                                 TCTTATTAACTTTGTCCATTAAGTCACCCTAATGTTTTACTTAATAAGCTAACGAATGAGCCACATCCGGGATAGCATCCGCCGATCTATTCGATCACTATCCTCCTCGTCTACAAATACATATATTGCACTCTATAAAGGCCACTCATATATTAACCTTTAATCTTCAAATACAAATATTTATTTGCACAGGCGCTTTAACTGTACTGCCGAACTTTCCCCCTTTCCATTAATCATTATTGTACAACGGTGTTGTT  >  CP000730/2272605‑2272861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑