Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 27.4% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT27.4% 255.4 / 72.5 129intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (45/45);  new base T (13/22);  total (60/69)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAA  >  CP000730/76041‑76320
                                                                                                                                           |                                                                                                                                            
gAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCt                                                                                                                                             <  1:978578/141‑1 (MQ=255)
    ttAATATAACTAATATTGCTGCACTTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAg                                                                                                                                         <  2:723052/141‑1 (MQ=255)
          taACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAAAATAGGTTCTGTTGCAAAGTTGAATCAAAAGTATAAt                                                                                                                                   >  2:881625/1‑141 (MQ=255)
           aaCTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAAGCTATAGTATAAtt                                                                                                                                  >  2:115202/1‑141 (MQ=255)
            aCTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAttt                                                                                                                                 >  1:84363/1‑141 (MQ=255)
               aaTATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTaa                                                                                                                              <  1:49591/141‑1 (MQ=255)
                 tatTGCTGCACCTGTCTCCCTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGGTGAATCTATAGTATAATTTTAACa                                                                                                                            >  1:515418/1‑141 (MQ=255)
                    tgctgcACCTGTATCACTTAGAACAGCCAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTCCGAATCAGTGGCTATAGTATAATTTTAACaaaa                                                                                                                         <  1:253745/141‑1 (MQ=255)
                       tgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAgg                                                                                                                      <  1:785109/141‑1 (MQ=255)
                          aCCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACGAAAAGGAGt                                                                                                                   >  1:969546/1‑141 (MQ=255)
                              gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                               <  2:255214/141‑1 (MQ=255)
                              gTATCACTGAGAACAGCTAACCAAAGTGTTAGGAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                               >  2:888675/1‑141 (MQ=255)
                               tATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttct                                                                                                              >  2:638263/1‑141 (MQ=255)
                                    cTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                         <  1:755341/141‑1 (MQ=255)
                                     ttAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCGATAGTATACTTTTAACGAAAGCGACTCTTCTGTATGa                                                                                                        >  1:929049/1‑141 (MQ=38)
                                             gCTAACCAAAGTGTTAGTAATCCAGGGAATACTGACACAAAGGCAATTAATTTAATAATTATAGCAAAATATTGGTTCTGTTGCAAAGTTGAATCTATAGTATAATATAAAAAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                <  2:592534/141‑1 (MQ=37)
                                             gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATTGGTTCTGTTGCAAAGTTGAATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                <  2:625975/141‑1 (MQ=37)
                                             gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAACGGAGTCTTCTGTATGAACTATTTc                                                                                                <  1:674516/141‑1 (MQ=255)
                                             gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                <  1:94099/141‑1 (MQ=255)
                                             gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                <  2:389928/141‑1 (MQ=255)
                                             gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                                <  2:390115/141‑1 (MQ=255)
                                               tAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAg                                                                                              <  1:894754/141‑1 (MQ=255)
                                                  ccAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGata                                                                                           >  2:826477/1‑141 (MQ=255)
                                                  acAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGata                                                                                           <  2:58707/140‑1 (MQ=255)
                                                     aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTATTGTATTTACTCTTTCAGATATaa                                                                                        <  1:1052631/141‑1 (MQ=37)
                                                          ttAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTCCTGTATGAACTATTTCAGATATAAACAAt                                                                                   >  2:407679/1‑141 (MQ=255)
                                                             gaaaTCCAGTGAAGTCTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTAGAGTATAATTTGAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTa                                                                                <  2:957087/139‑1 (MQ=21)
                                                              tAATCCAGGAAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTaa                                                                               <  2:366389/141‑1 (MQ=255)
                                                                aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAGACCCTATTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACa                                                                             <  1:295472/141‑1 (MQ=18)
                                                                   cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAgg                                                                          <  1:571626/141‑1 (MQ=255)
                                                                      ggAATGCTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                       >  1:942234/1‑141 (MQ=255)
                                                                      ggAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGCTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATg                                                                       >  2:420150/1‑141 (MQ=255)
                                                                       gAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGt                                                                      <  2:213904/141‑1 (MQ=255)
                                                                        aaTACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGtt                                                                     <  1:1035491/141‑1 (MQ=255)
                                                                        aaTACAAAAACAAAGGAAATTAATTTAATAATTAAAGCAAAATATAGGTTCTATTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGtt                                                                     <  2:581376/141‑1 (MQ=18)
                                                                          tACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTAt                                                                   >  1:93780/1‑141 (MQ=255)
                                                                              aaCACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGGTATAAACAATTTAACAAGGATGTTATCACt                                                               >  1:1019330/1‑141 (MQ=255)
                                                                                cacaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGt                                                             <  1:777444/141‑1 (MQ=255)
                                                                                 acaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                            <  1:287562/141‑1 (MQ=255)
                                                                                 acaAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTa                                                            <  1:26806/141‑1 (MQ=255)
                                                                                      ggCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGt                                                       >  1:1054431/1‑141 (MQ=255)
                                                                                      ggCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGCAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTACCCGt                                                       >  1:222747/1‑141 (MQ=255)
                                                                                              atttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                               <  2:645849/141‑1 (MQ=255)
                                                                                               tttaatAATTATAGTAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctacta                                              <  1:881625/141‑1 (MQ=255)
                                                                                                      atTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGTTACTATCTATGa                                       >  1:301605/1‑141 (MQ=255)
                                                                                                         ataGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGatat                                    <  2:553049/141‑1 (MQ=255)
                                                                                                         ataGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTGCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATGACTGTAGCCATTGGCTACTATCTAAGatat                                    >  2:966663/1‑141 (MQ=255)
                                                                                                          taGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTCGGCTACTATCTAAGATATg                                   <  1:582823/141‑1 (MQ=255)
                                                                                                           aGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGc                                  >  1:930850/1‑141 (MQ=255)
                                                                                                            gCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCa                                 >  2:986251/1‑141 (MQ=255)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                <  2:1058872/133‑1 (MQ=11)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                >  1:284678/9‑141 (MQ=1)
                                                                                                             cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGACTTCTGTGTGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                                >  1:939368/9‑141 (MQ=11)
                                                                                                              gtaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCAtt                               <  1:1215/134‑1 (MQ=255)
                                                                                                               taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                              <  2:89468/135‑1 (MQ=255)
                                                                                                               gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtta                              >  2:255221/7‑140 (MQ=1)
                                                                                                               gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                              <  2:949694/135‑1 (MQ=255)
                                                                                                               gaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                              <  1:731546/135‑1 (MQ=255)
                                                                                                               aaaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                              <  1:601927/135‑1 (MQ=9)
                                                                                                                ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                             <  2:290255/136‑1 (MQ=9)
                                                                                                                ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                             >  1:450104/6‑141 (MQ=0)
                                                                                                                ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                             <  2:956378/136‑1 (MQ=9)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                            <  2:625874/137‑1 (MQ=9)
                                                                                                                 taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                            <  1:810736/137‑1 (MQ=9)
                                                                                                                 atatAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAACCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                            <  2:34543/141‑1 (MQ=255)
                                                                                                                 actgaGGGTCTGTTGCAAAGTTGAATTTATAGTAAAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            <  1:40967/137‑1 (MQ=11)
                                                                                                                 aatgaGGTTCTGTTGGAACGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            <  1:332755/137‑1 (MQ=11)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            <  2:312160/137‑1 (MQ=9)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            >  2:72705/5‑141 (MQ=0)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            >  1:1012053/5‑141 (MQ=0)
                                                                                                                 aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            >  2:295921/5‑141 (MQ=2)
                                                                                                                 aaaaaagttcaattGCAAAATTAAATATAAAGTAAAATTTCAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAATTAACAAGAATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                            <  1:50499/129‑1 (MQ=11)
                                                                                                                  ctacagatCTGTTGGAAGATGGAATTTATAATATAATTTTAACAGAAAGGAGTCTTCTGTATGAAATATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGACTACTATCTAAGATATGCATTGAgc                           >  2:96666/8‑140 (MQ=1)
                                                                                                                  atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                           <  1:849692/138‑1 (MQ=2)
                                                                                                                  atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                           <  1:798276/138‑1 (MQ=2)
                                                                                                                  aggaGGTTCTGTTGCAAAGTTGAAGTTCTAATATAATGTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCTGATATAAACAGTTGGACACGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgc                           >  2:113349/4‑140 (MQ=11)
                                                                                                                   tgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGtt                          <  2:194631/139‑1 (MQ=2)
                                                                                                                   ggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgct                          >  1:352593/3‑139 (MQ=0)
                                                                                                                    gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACACGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGttt                         >  1:554661/2‑140 (MQ=0)
                                                                                                                     aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGGTACCCTCAAAGATATGCATTGAGTTAt                        >  2:1063746/1‑141 (MQ=11)
                                                                                                                     aGGTTCTGTTGCAAAGTTGAATGTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                        >  2:775959/1‑141 (MQ=18)
                                                                                                                      ggTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATc                       >  2:246418/1‑141 (MQ=35)
                                                                                                                       gTTCTGTTGAAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCg                      <  1:22405/141‑1 (MQ=25)
                                                                                                                        gtgtggtGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGt                     <  2:520859/135‑1 (MQ=0)
                                                                                                                               gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat              >  1:272592/1‑141 (MQ=35)
                                                                                                                               gCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat              >  2:196331/1‑141 (MQ=34)
                                                                                                                                cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc             >  1:894517/1‑141 (MQ=35)
                                                                                                                                   aGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGAAGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa          >  2:376761/1‑141 (MQ=25)
                                                                                                                                    gTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTGAGATATGCATTGAGTTATCGTGATATATCTGaa         >  1:720361/1‑141 (MQ=25)
                                                                                                                                    gTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa         <  2:1053453/141‑1 (MQ=35)
                                                                                                                                    attGAAATTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGaa         <  1:907025/140‑1 (MQ=255)
                                                                                                                                      tGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAat       >  1:101427/1‑141 (MQ=35)
                                                                                                                                        aaTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGATTTATCGTGATATATCTGAAatat     >  2:86914/1‑141 (MQ=25)
                                                                                                                                        aaTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAatat     >  2:802261/1‑141 (MQ=35)
                                                                                                                                        aaTCTATAGTATAATTTTAACAAAAAGGAGTCTACTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCGGAAaaat     >  2:530759/1‑141 (MQ=255)
                                                                                                                                         aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTGCTATCTAATATATGCTTTGAGTTATCGTGATATATTTGAattatt    >  1:432854/1‑141 (MQ=11)
                                                                                                                                         aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt    >  2:787245/1‑141 (MQ=35)
                                                                                                                                         aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATAtt    >  2:257440/1‑141 (MQ=34)
                                                                                                                                          tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATCAACAATTTAACAAGGATGTTATCACTGTAGCCGGTGGCTACGATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa   <  1:615357/141‑1 (MQ=16)
                                                                                                                                           cTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTaa  <  1:797561/141‑1 (MQ=33)
                                                                                                                                           |                                                                                                                                            
GAATTTAATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAA  >  CP000730/76041‑76320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: