Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 396,540 G→A 85.9% A208T (GCA→ACA)  USA300HOU_0364 → possible lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730396,5400GA85.9% 263.4 / 31.1 113A208T (GCA→ACA) USA300HOU_0364possible lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (9/7);  new base A (54/43);  total (63/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01

Only 100 of 114 total aligned reads displayed.

ACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAG  >  CP000730/396402‑396674
                                                                                                                                          |                                                                                                                                      
aCATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGcaca                                                                                                                                      >  1:631387/1‑141 (MQ=255)
          tCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTGAAGCGAACGTTGAAGGCACACAAAAAAttt                                                                                                                            >  2:67381/1‑141 (MQ=255)
          tCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAttt                                                                                                                            >  1:119173/1‑141 (MQ=255)
          tCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAAttt                                                                                                                            >  2:805168/1‑141 (MQ=255)
          tCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAAAGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAAttt                                                                                                                            <  2:506341/141‑1 (MQ=255)
              aacAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                        <  1:163954/139‑1 (MQ=255)
              aCCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGa                                                                                                                        <  1:540105/141‑1 (MQ=255)
               ccAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGAc                                                                                                                       <  1:127053/141‑1 (MQ=255)
                cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGACGGCACACAAAAAATTTATGACt                                                                                                                      >  2:856861/1‑141 (MQ=255)
                cAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                      <  1:863615/141‑1 (MQ=255)
                cAAAATTAATGTTACAAGGTGTTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                      <  1:74834/141‑1 (MQ=255)
                cAAAATTAATGTTACAAGGCTATGTTAACCTAGCAAATGACGTTGGCAATCCCAAAATCACAGGTGAAGCAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACt                                                                                                                      <  1:766635/141‑1 (MQ=255)
                  aaaTTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTa                                                                                                                    <  2:290626/141‑1 (MQ=255)
                      tAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTa                                                                                                                >  1:408856/1‑141 (MQ=255)
                        aTGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTaaa                                                                                                              <  1:689834/141‑1 (MQ=255)
                         tGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGCGTTATTTAAAc                                                                                                             >  1:698061/1‑141 (MQ=255)
                          gTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAcc                                                                                                            >  1:662031/1‑141 (MQ=255)
                          gTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAAcc                                                                                                            <  1:336554/141‑1 (MQ=255)
                           ttACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCt                                                                                                           >  2:719306/1‑141 (MQ=255)
                             aCAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTAt                                                                                                         <  2:364782/141‑1 (MQ=255)
                               aaGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTAttt                                                                                                       <  2:410068/141‑1 (MQ=255)
                               aaGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTAttt                                                                                                       <  1:640070/141‑1 (MQ=255)
                                 gtttCTGTGGACATATTAAATGAAGTTGCAACTTTTAAAATAATATGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCTAACGTTGAAGTCACACAAAAAATTTATGACTTATTTAAACCTATTTTa                                                                                                     <  2:527967/139‑1 (MQ=255)
                                   ttCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTaga                                                                                                   >  1:866668/1‑141 (MQ=255)
                                   ttCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTaga                                                                                                   >  2:555967/1‑141 (MQ=255)
                                    tCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGACCGTTGAAGGCACACAAAAAATTTATGACTTATTTAACCCTATTTTagag                                                                                                  >  1:665317/1‑141 (MQ=255)
                                     cTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAgaga                                                                                                 <  1:651182/141‑1 (MQ=255)
                                       gTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaa                                                                                               >  2:1063403/1‑141 (MQ=255)
                                       gTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaa                                                                                               >  1:377669/1‑141 (MQ=255)
                                       gTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCCAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGaaa                                                                                               >  2:495367/1‑141 (MQ=255)
                                       gTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATAAAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATATTAGAGaaa                                                                                               >  1:596483/1‑141 (MQ=255)
                                            ccTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGa                                                                                          >  1:627197/1‑141 (MQ=255)
                                               aTTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATaa                                                                                       >  2:830416/1‑141 (MQ=255)
                                                  aaaTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTACACCTATTTTAGAGAAAAAAGATaaaaa                                                                                    <  2:620710/141‑1 (MQ=255)
                                                  aaaTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATaaaaa                                                                                    >  2:69633/1‑141 (MQ=255)
                                                    aTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                  >  2:868730/1‑141 (MQ=255)
                                                    aTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                  >  1:357902/1‑141 (MQ=255)
                                                    aTGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAt                                                                                  >  1:406740/1‑141 (MQ=255)
                                                     tGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAtt                                                                                 <  2:45362/141‑1 (MQ=255)
                                                     tGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAtt                                                                                 <  1:858911/141‑1 (MQ=255)
                                                     agAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAtt                                                                                 <  1:516078/140‑1 (MQ=255)
                                                     agAAGTTGAAACTACTAAAAACACAGGGGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTAAAAAAAAAAAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAtt                                                                                 <  2:190079/140‑1 (MQ=255)
                                                     agAAGTTAGAACGTCTAAAATCACAAGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAAGAAAACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAAtt                                                                                 <  2:821550/140‑1 (MQ=255)
                                                      gAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATCCAGATTTATATGATTTTAAAACGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAAATAAAAAATTa                                                                                >  1:352212/1‑141 (MQ=255)
                                                      gAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTa                                                                                >  1:740332/1‑141 (MQ=255)
                                                       aaGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGATAAAAAAGATAAAAAATTaa                                                                               >  1:146594/1‑141 (MQ=255)
                                                       aaGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTaa                                                                               >  2:649039/1‑141 (MQ=255)
                                                            gCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtgaa                                                                          >  2:454152/1‑140 (MQ=255)
                                                            gCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGtaat                                                                          >  2:656466/1‑141 (MQ=255)
                                                               aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTgatgat                                                                       >  2:132183/1‑141 (MQ=255)
                                                               aCTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAACATAAAAAATTAAGTgataat                                                                       >  1:448546/1‑141 (MQ=255)
                                                                 atcaaaaaTAAAAAGTGAAGAAGAAATTAATTCACATACAGATATAGATGATTTTAAAGCGAACGTTAAAAGCAAACAAAAAATATATGACTTATTGAAAAAAAAAAAAAAAAAAAAAGATAAAAAATTAAGTGATGatat                                                                     <  2:140372/137‑1 (MQ=255)
                                                                  tCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGAAAAAAAATAAAGTGATGATATc                                                                    >  2:1016481/1‑141 (MQ=255)
                                                                  tCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGAGTTATATGATTTTAAAGCGAACGTTGAAGGAACACAAACAATGTATGACTTATTTAAACCTATTTTAGAAAAAAAAGAGAAAAAATTAAGTGTAGatata                                                                    >  2:927137/1‑140 (MQ=255)
                                                                     aaaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaa                                                                 <  2:558112/141‑1 (MQ=255)
                                                                      aaaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaaa                                                                <  1:88609/141‑1 (MQ=255)
                                                                      aaaCCACAGGTGAAGAAAAAAATTAAACTCATAGAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCaaa                                                                <  1:713212/141‑1 (MQ=255)
                                                                       aaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAAt                                                               >  2:552163/1‑141 (MQ=255)
                                                                       aaTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGAGTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAAt                                                               <  1:456657/141‑1 (MQ=255)
                                                                        aTCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATg                                                              <  2:323885/141‑1 (MQ=255)
                                                                           acaGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCAACATGAAc                                                           >  2:262852/1‑141 (MQ=255)
                                                                           acaGGACAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAAc                                                           >  2:888567/1‑141 (MQ=255)
                                                                              ggTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTc                                                        <  1:597204/141‑1 (MQ=255)
                                                                              ggTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTc                                                        <  1:447447/141‑1 (MQ=255)
                                                                               gTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCg                                                       >  1:432141/1‑141 (MQ=255)
                                                                                 gaagaagaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGAt                                                     >  1:706970/1‑141 (MQ=255)
                                                                                      agaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGt                                                >  1:971241/1‑141 (MQ=255)
                                                                                      agaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGt                                                <  1:813244/141‑1 (MQ=255)
                                                                                       gaaATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTg                                               >  2:617376/1‑141 (MQ=255)
                                                                                         aattaattCACATACAGATTTATATAAATTTAAAACGAACCATAAACACACACAAAAAATTTATTACTTATTTAAAACAATAAAAAAAAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGaa                                             <  2:221641/141‑1 (MQ=255)
                                                                                            ttATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGTACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCa                                          <  2:51428/141‑1 (MQ=255)
                                                                                              aTTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCact                                        >  1:215224/1‑139 (MQ=255)
                                                                                                tCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAatta                                      >  2:1058236/1‑141 (MQ=255)
                                                                                                 cacaTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAattat                                     <  2:22486/141‑1 (MQ=255)
                                                                                                 cacaTACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAattat                                     <  2:386289/141‑1 (MQ=255)
                                                                                                   caTACCGATGTATATGATTTTAAAGCGAACGTTGAAGACACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTg                                   <  2:160896/141‑1 (MQ=255)
                                                                                                        aGATTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATaa                              <  1:315287/141‑1 (MQ=255)
                                                                                                          aTTTATATGATTTTATAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAat                            >  1:788750/1‑141 (MQ=255)
                                                                                                          aTTTATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAat                            >  1:37429/1‑141 (MQ=255)
                                                                                                           tttATATGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAata                           >  1:886440/1‑141 (MQ=255)
                                                                                                               tatGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaaa                       >  1:332730/1‑141 (MQ=255)
                                                                                                               tatGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATaaa                       >  2:732235/1‑141 (MQ=255)
                                                                                                                atGATTTTAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAg                      <  2:631387/141‑1 (MQ=255)
                                                                                                                  gATTTTAAAGCGAACGTTGAAGGCACACAAGAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGAt                    >  1:894879/1‑141 (MQ=255)
                                                                                                                      ttAAAGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaaca                >  2:535478/1‑141 (MQ=255)
                                                                                                                       tAAAGCGAACGTTGAAGGCACACAAAAAAACTCGAGCGAATTTATACCTCTTTAAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaa               >  2:748565/1‑141 (MQ=255)
                                                                                                                        aaaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATaacaac              >  1:367549/1‑141 (MQ=255)
                                                                                                                         aaGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTGAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTGGATAAAGTGAATCAATTATTGGATAAATATAAAGATAAAAAcg             >  2:76741/1‑141 (MQ=255)
                                                                                                                          aGCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcgg            <  1:555967/141‑1 (MQ=255)
                                                                                                                           gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAACTGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggc           >  1:933945/1‑141 (MQ=255)
                                                                                                                           gCGAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTGCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggc           >  2:546437/1‑141 (MQ=255)
                                                                                                                             gAACGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAAcggcgg         <  2:714035/141‑1 (MQ=255)
                                                                                                                              aaCGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAACGTTAAGTGATGATATCGAAATGAACTTAGATAAAGTGTATGGATTGTTGGATAATTATGAAGATAACAATGGAGGt        >  1:289851/1‑141 (MQ=255)
                                                                                                                              aaCGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATTAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGt        <  1:830416/141‑1 (MQ=255)
                                                                                                                                cGTTGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATTTCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTa      <  2:170163/141‑1 (MQ=255)
                                                                                                                                 gTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTAt     >  2:485288/1‑141 (MQ=255)
                                                                                                                                  ttGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTAATg    >  1:813122/1‑141 (MQ=255)
                                                                                                                                  ttGAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATg    >  2:681249/1‑141 (MQ=255)
                                                                                                                                    gAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAg  >  2:769662/1‑141 (MQ=255)
                                                                                                                                    gAAGGCACACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAg  >  1:232248/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                      
ACATTAGATATCATACCAAAATTAATGTTACAAGGTTCTGTTGACCTATTAAATGAAGTTGCAACTTCTAAAATCACAGGTGAAGAAGAAATTTATTCACATACAGATTTATATGATTTTAAAGCGAACGTTGAAGGCGCACAAAAAATTTATGACTTATTTAAACCTATTTTAGAGAAAAAAGATAAAAAATTAAGTGATGATATCCAAATGAACTTCGATAAAGTGAATCAATTATTGGATAAATATAAAGATAACAACGGCGGTTATGAG  >  CP000730/396402‑396674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: