Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 602,466 G→T 9.2% intergenic (+56/‑101) hchA → / → araB possible hydrolase/ribulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730602,4660GT 9.2% 233.9 / 4.1 91intergenic (+56/‑101)hchA/araBpossible hydrolase/ribulokinase
Reads supporting (aligned to +/- strand):  ref base G (31/49);  new base T (7/2);  total (39/52)
Fisher's exact test for biased strand distribution p-value = 3.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGAATTGATTATGGAACTGCTT  >  CP000730/602330‑602606
                                                                                                                                           |                                                                                                                                            
aCAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCaa                                                                                                                                          <  1:960300/141‑1 (MQ=255)
aCAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCaa                                                                                                                                          >  2:52966/1‑141 (MQ=255)
aCAGGATACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCTGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGTCaa                                                                                                                                          <  2:545035/141‑1 (MQ=255)
 cAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGTCaaa                                                                                                                                         >  2:434136/1‑141 (MQ=255)
 cAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTGGCaaaa                                                                                                                                        >  2:17012/1‑141 (MQ=255)
     tGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAAttt                                                                                                                                     <  2:627163/141‑1 (MQ=255)
       aCAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTGGCAAAATCTAAg                                                                                                                                  <  1:722252/141‑1 (MQ=255)
        cAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAg                                                                                                                                  >  2:139078/1‑141 (MQ=255)
            ccTTTAGCTTAAAATGAGTTAGGAAAATTAACAATTAATGAAATGTTAAATGAAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATTTATGAGGTCTCTTTTTTTTTGGCAAAATTTAAGTGAt                                                                                                                              <  2:76347/141‑1 (MQ=255)
            actatAGCTTAAAAAGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGATGACCCGCAAATGTAAACGTATTATGGCTCTTTTTTTTTGGCAAAATTTAAGGGAt                                                                                                                              <  2:798415/137‑1 (MQ=255)
                tAGCTTCAAATGAGGTAGGAAAATTAGCCGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACtt                                                                                                                          <  2:934789/141‑1 (MQ=255)
                 atcTTCAAATGATTTAGAAAAATTAGCTGTTAATGAAATGTTAAAAGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGTCAAAATTTAAGTGATACTTg                                                                                                                         <  2:981465/139‑1 (MQ=255)
                 aGCTTCAAATGAGCTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTg                                                                                                                         <  2:763358/141‑1 (MQ=255)
                  gCTTAAAATGAGTTAGGAAAATTAGCAGTTAATGAATTGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGt                                                                                                                        <  1:696234/141‑1 (MQ=255)
                  gCTTAAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGt                                                                                                                        <  2:63222/141‑1 (MQ=255)
                     tCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTaaa                                                                                                                     <  2:921067/141‑1 (MQ=255)
                     tCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTaaa                                                                                                                     <  1:782705/141‑1 (MQ=255)
                     ataaaagagtaacaaaaaTTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTAATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTaaa                                                                                                                     <  1:735300/127‑1 (MQ=255)
                     acAAATGAGTTAGGAAAATTAGAGGTTAATTAAATGTCAAACGCAATACAAAATAAATAATAAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTaaa                                                                                                                     <  1:576521/140‑1 (MQ=255)
                       aaaTGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAAGTAAATATTAATTAGAGGAGCCTCATATGTAAAAGTATGAGTGCAATTTTTTTTTGGGAAAATTTGAGTGATACTTGTaacaa                                                                                                                   >  2:886736/1‑138 (MQ=255)
                          tGAGTTAAGTAAATTATCAGTTAATGAAATGTTAAATGCAAAAAAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTTGCAAAATTTAAGTGATACTTGTAAAATAGa                                                                                                                <  2:834124/141‑1 (MQ=255)
                            aGTAAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAACAATTAAATATTACTTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATATTTGTAAAATAGAAc                                                                                                              <  2:725457/141‑1 (MQ=255)
                             gTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCACAAATTAAGTGATACTTGTAAAATAGaaac                                                                                                             >  1:648168/1‑139 (MQ=255)
                             gTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAAcc                                                                                                             >  1:834551/1‑141 (MQ=255)
                             gTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAAcc                                                                                                             >  1:696183/1‑141 (MQ=255)
                             gTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATCAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAAcc                                                                                                             >  1:146259/1‑141 (MQ=255)
                                aGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGTAAAATTTAAGTGATACTTGTAAAATAGAACCtct                                                                                                          >  2:734019/1‑139 (MQ=255)
                                  gAAAATTAGCGGTTAATGAAATGTCAAATGCAATACAAAATCAATCATAAACGTTTTAATTTGGGAGGCATTAGAGAAAATGTATGCGGCCTCTTTTTTTTTGGCAAATTTGGAGTGATGCTTGTAAAATAGAACCtatta                                                                                                        >  1:775912/1‑141 (MQ=255)
                                  gAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCtatta                                                                                                        >  1:694492/1‑141 (MQ=255)
                                   aaaaTTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCtattat                                                                                                       <  2:1021505/141‑1 (MQ=255)
                                     aaTGAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGa                                                                                                     <  2:474891/141‑1 (MQ=255)
                                       ttAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGt                                                                                                   >  2:978468/1‑141 (MQ=255)
                                        aaGCAGATAATGAAACATTAAACACACAACAAAACAAATAATTAAATATAAATTAGAGGAACCTAATATGTAAATGAATGAGAGCTCTCTTTTTTTCGCAAAATTTAAGTGATACTTGTATAATAGAACCTATTATGAGTa                                                                                                  <  1:645050/140‑1 (MQ=255)
                                         agtaGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCATAGTTTAACTGATACTTGTAAAATAGAACCTATTATGACTAt                                                                                                 >  2:145290/4‑141 (MQ=255)
                                         aGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATACTAATTAGAGGAGCCTCATATGTACATGTCTGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTACAATAGCACCTATTATAGGTAt                                                                                                 >  1:1011428/1‑141 (MQ=255)
                                           cAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGa                                                                                               >  1:938967/1‑141 (MQ=255)
                                           cAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGa                                                                                               >  2:938517/1‑141 (MQ=255)
                                             ataaagaaaaTATAAAAAGCAAAAAAAAATAAAAAATTAAATATTAATTAGAGGAGACTAATATGTAAATGTATGAGGGCTCTTTTTTTTTTTGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGAt                                                                                              <  2:860734/134‑1 (MQ=255)
                                               taagaaaaTGTTAAATGCAATACAAAATAAATAATTAAATATTATTTAAAGGATTCTCAAATGTAAATTTATGAGGGTTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTa                                                                                           <  2:690707/136‑1 (MQ=255)
                                                         ttAAATGCAATTAAAAATAAATAATTAAATATTAATTAGAGGAGACTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCt                                                                                 <  1:742245/141‑1 (MQ=255)
                                                         ttAAATGCAATACAAAATAAATAATTAAATATGAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCt                                                                                 <  2:54414/141‑1 (MQ=255)
                                                         ttAAATGAAAAACAAAATAAATAATTAATCATTAATTAACCGGGGGATCGTGTGAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCt                                                                                 <  1:1037398/141‑1 (MQ=255)
                                                            aaTGCAATACAAAATAAATAATTAAAGATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGc                                                                              <  2:264744/141‑1 (MQ=255)
                                                                  aTACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACt                                                                        <  2:499336/141‑1 (MQ=255)
                                                                   aaaaaaaaaaaaaATTAAAAATTAATTAAAGGAGCCTCATATGTAAATGTATGAGAGCTCGTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACta                                                                       <  2:526625/138‑1 (MQ=255)
                                                                       aaataaatAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTTGCAAAATTTAAGTGATACCTGGAAAATAGAACCTATTCTGATTATGATTTAAGAAACCGCTTGccaccctaacac                                                                   >  2:461145/1‑130 (MQ=255)
                                                                        aataaataATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAAc                                                                  >  1:849119/1‑141 (MQ=255)
                                                                          taaataaTTAAATATTAATAAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTATTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTACGATTTAAGAAAACGCTTGCATAACTAATAAcac                                                                >  2:593747/1‑139 (MQ=255)
                                                                           aaataaatAAAAATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACCCTTGCAAAACTAATAACCGc                                                               <  1:643457/141‑1 (MQ=255)
                                                                           aaataaTTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTTGCAAATTTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAACACGCTTCCAAAACTAATAACCGc                                                               >  2:40105/1‑141 (MQ=255)
                                                                           aaataaTTAAATAATAATTAGAGGACCCTCATATGTAAATGTATGAGGGCTATTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGc                                                               <  2:590986/141‑1 (MQ=255)
                                                                                 aaaaaaATTAATTAGAGGAGACTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAg                                                         <  1:1063230/139‑1 (MQ=255)
                                                                                  aaaaaATAAATAAAAGGAGCCTAAAATGTAAAGGTATGAGGGCTTTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGc                                                        <  1:1059292/140‑1 (MQ=255)
                                                                                         ataattaGAGGGGCGTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATg                                                  <  2:123415/140‑1 (MQ=255)
                                                                                         ataattaGAGGAGCCTCATATGTAAATGTATGAGGGCTTTTTTTTTTTGGCAAAATTGAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATg                                                  <  1:512192/140‑1 (MQ=255)
                                                                                           aattaGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTACTATTTTTTTAGTGCTGTATGTAAAATAGACCCTTTTTTTACTATGTTTAATGAATACGCTTTCAAAACTAATAACCCCAACTAGATATGTgga                                                >  2:820553/1‑141 (MQ=255)
                                                                                             ttaGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGTCAAAATTTAAGTGATACATGTAAAATAGAACCTATTATGAATATAATTTAAGAAAACGCCTCCAGAACTCATAACCGCAACTAACGATATgtaag                                              >  2:874642/1‑137 (MQ=255)
                                                                                                  gggagCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAAc                                          <  2:379679/140‑1 (MQ=255)
                                                                                                  aggagCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTTTTTTTTTTTATATAATACGTGTAAAATAGAACCTATTATGAGGATGATTTATGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGGTATGGAGGaaaa                                          >  2:752803/1‑140 (MQ=255)
                                                                                                  aggagCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACGGCAACTAGCGATATGGGGGaaaa                                          >  1:27970/1‑140 (MQ=255)
                                                                                                  aggagCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAAc                                          >  1:348481/1‑141 (MQ=255)
                                                                                                   ggagCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTAAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACa                                         >  1:411646/1‑141 (MQ=255)
                                                                                                   ggagCCTCATATGTAAATGTATGAGGGCTCTTTTTTAAAGTCTAAATTTACGTGTTTCGTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACa                                         >  1:509840/1‑141 (MQ=255)
                                                                                                   ggagCCAAATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACa                                         <  1:621774/141‑1 (MQ=255)
                                                                                                   ggagCCAAATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACa                                         <  2:11660/141‑1 (MQ=255)
                                                                                                     agCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTTTTTTTTTTTAGGTGATACTTGTAAAATAAAACCTATTATGTGAATGGTTTAAGAAAACGCTTCCACACCTAATAACCGCAACTAGCGCTCTAGGAGAAACatg                                       >  2:397221/1‑141 (MQ=255)
                                                                                                     agCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATAAGGAGGAAACatg                                       >  1:297636/1‑141 (MQ=255)
                                                                                                        ctaatatGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACatgatg                                    <  2:807546/138‑1 (MQ=255)
                                                                                                           atatGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCt                                 <  2:938967/141‑1 (MQ=255)
                                                                                                           atatGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGGGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCt                                 <  2:397744/141‑1 (MQ=255)
                                                                                                            tatGTAAATGTATGAGGGCTCTTTTTTTTTGGCTAAATTTAAGTGATACGTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTACAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCtt                                <  1:719459/141‑1 (MQ=255)
                                                                                                              tGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGTAAACATGATGTCTtat                              <  2:383292/141‑1 (MQ=255)
                                                                                                               gTAAATGTAGGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTtata                             <  1:229840/141‑1 (MQ=255)
                                                                                                                tAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAACCTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTTTAg                            >  2:56668/1‑141 (MQ=255)
                                                                                                                  aaTTTATAAGGGCTTTTTTTTTTTGGCAAAATTTAAGTGATACGTGTAAAATAGAAACTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCa                          <  1:689564/141‑1 (MQ=255)
                                                                                                                  aaTGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCa                          >  1:591915/1‑141 (MQ=255)
                                                                                                                  aaTGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCa                          <  1:689722/141‑1 (MQ=255)
                                                                                                                   aTGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCAt                         <  1:938517/141‑1 (MQ=255)
                                                                                                                   aTGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTAAAGCAt                         >  1:611483/1‑141 (MQ=255)
                                                                                                                       aTGAGGGCTCTTTTTTTTTTGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTCGa                     >  2:248289/1‑141 (MQ=255)
                                                                                                                       aTGAGGGCACATTTTTTTAGGCAAAAATTAAGTGATACTTGTAAAAAAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGa                     <  1:17944/141‑1 (MQ=255)
                                                                                                                        tGAGGGATATTTTTTTTTGGAAAACTTTAGGTCAAGCATGAAAAATATAACCAAAAATAGCTAACATAGAAAAAAACGCTTCGAAAACAAATAACCGCAACTAACGAGATGGAGGAAACATGATGTCTTATAGCATTGGaa                    <  2:883107/141‑1 (MQ=255)
                                                                                                                         gagaGCACTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGAAt                   <  1:360275/141‑1 (MQ=255)
                                                                                                                         aaGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGAAt                   <  1:360323/140‑1 (MQ=255)
                                                                                                                             gCTCTTTTTTTTTTTTTAAATTTAAGTGATACTTGTAAAATATAACCTTTTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTATCGATATGGAGGGAACATGATGTCTTATAGCATTggtagttat               >  2:956491/1‑134 (MQ=255)
                                                                                                                              ctctTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGGTTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGCTATGGAGGAAACATGATGTCTTATAGCATAGGAATTGAtt              >  1:45047/1‑141 (MQ=255)
                                                                                                                               tctTTTTTTTTTTCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGAATTGATTa             >  2:75393/1‑141 (MQ=255)
                                                                                                                                  tttttttttGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGAATTGATTATg           <  2:849345/141‑1 (MQ=255)
                                                                                                                                   ttttttttGGCAGAATTTAAGTGATACTTGTAAAATAGAACCTATTATGGGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATCGAGGAAACATGATGTCTTATAGCATTGGAATTGATTATgg          >  1:750705/1‑141 (MQ=255)
                                                                                                                                   ttttttttGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGAATTGATTATgg          >  1:312424/1‑141 (MQ=255)
                                                                                                                                    tttttatGACAAAAATTAAGTGAAAATTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAAATAATAACCGCAACTAGTGATATGGAGGAAACATGATGTCTTACAGCACTGGAATTGATTATGGa         <  1:544683/141‑1 (MQ=255)
                                                                                                                                           ggCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGAATTGATTATGGAACTGCtt  >  1:224115/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                            
ACAGGTGACAGTCCTTTAGCTTCAAATGAGTTAGGAAAATTAGCAGTTAATGAAATGTTAAATGCAATACAAAATAAATAATTAAATATTAATTAGAGGAGCCTCATATGTAAATGTATGAGGGCTCTTTTTTTTTGGCAAAATTTAAGTGATACTTGTAAAATAGAACCTATTATGAGTATGATTTAAGAAAACGCTTGCAAAACTAATAACCGCAACTAGCGATATGGAGGAAACATGATGTCTTATAGCATTGGAATTGATTATGGAACTGCTT  >  CP000730/602330‑602606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: