Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 863,781 A→G 5.9% intergenic (+310/‑30) eno → / → USA300HOU_0807 phosphopyruvate hydratase/hypothetical membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730863,7810AG 5.9% 229.4 / 3.2 84intergenic (+310/‑30)eno/USA300HOU_0807phosphopyruvate hydratase/hypothetical membrane protein
Reads supporting (aligned to +/- strand):  ref base A (44/33);  new base G (2/3);  total (48/36)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

ACTAATAATCATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATATTATTGATTATTCATTACTTTGTTTCGTTGGATAATGC  >  CP000730/863642‑863920
                                                                                                                                           |                                                                                                                                           
aCTAATAATCATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGaa                                                                                                                                            <  2:428084/141‑1 (MQ=255)
     taatCATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGGACGTAt                                                                                                                                       >  1:565609/1‑141 (MQ=255)
     taatCATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGGACGTAt                                                                                                                                       >  1:1012487/1‑141 (MQ=255)
       atCATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTAttt                                                                                                                                     >  1:313100/1‑141 (MQ=255)
       atCATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTAtta                                                                                                                                     >  1:376895/1‑140 (MQ=255)
        tCATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATACATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGTTTTTATAGTAAGTCTTTa                                                                                                                                    >  2:52334/1‑141 (MQ=255)
        acATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTa                                                                                                                                    <  1:393718/140‑1 (MQ=255)
           tGAGGTAACTAATAAAACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTCTTGAACGTATTTAAat                                                                                                                                 <  1:589561/141‑1 (MQ=255)
             agtgaacgtataataCATATTTAACTTGTATTCTTAAACCGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGGACGTATTTAAatat                                                                                                                               <  2:263593/132‑1 (MQ=255)
             aGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGTGTATTCACTTTTATTAGAAGGTAAAAATAAATAAAGGTTTTATAGAACGTATTTAAatat                                                                                                                               >  2:348866/1‑141 (MQ=255)
             aGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAatat                                                                                                                               >  1:569464/1‑141 (MQ=255)
             aGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAatat                                                                                                                               >  1:892258/1‑141 (MQ=255)
               gTAACTAATAACACATATTTAACTTGTATTCTAATCCTGTTTAAATTGATCCATATTTAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAatatat                                                                                                                             <  1:81196/141‑1 (MQ=255)
                    taataaCACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATgagag                                                                                                                        >  1:229066/1‑139 (MQ=255)
                     aataaCACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGCATATTGTATGACAGGGTATTCGCTTATATTCAAGTGTAAAATTAAATAAAGGTTTTATAGAACGTACGTAActataaacggaa                                                                                                                       >  2:915993/1‑134 (MQ=255)
                      ataaCACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGt                                                                                                                      >  1:123772/1‑141 (MQ=255)
                         acacATATTTAAGTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTaaa                                                                                                                   <  1:823936/141‑1 (MQ=255)
                           acaAATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACa                                                                                                                 <  1:169958/141‑1 (MQ=255)
                            aatatTTAACTTGTATTCTTAAACTGGTATAATAAATTGATGTTGCACCAATAATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACaa                                                                                                                <  1:1038032/140‑1 (MQ=255)
                              tatTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACaaag                                                                                                              >  2:750079/1‑140 (MQ=255)
                                 ttAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGc                                                                                                           >  2:473171/1‑141 (MQ=255)
                                    aCTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGa                                                                                                        >  2:475997/1‑141 (MQ=255)
                                    aCTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGa                                                                                                        <  1:842894/141‑1 (MQ=255)
                                    aCTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGa                                                                                                        >  2:131847/1‑141 (MQ=255)
                                     cTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGAt                                                                                                       <  2:522918/141‑1 (MQ=255)
                                     cTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAAAAAATGGCTGAt                                                                                                       >  2:160135/1‑141 (MQ=255)
                                     attGTATTTTTAAAATGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTAATTAAAAGTGAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGAt                                                                                                       <  1:144906/140‑1 (MQ=255)
                                       tGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAg                                                                                                     >  1:986825/1‑141 (MQ=255)
                                         tATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGATGAGTAAACAAATGGCTGATAGaa                                                                                                   >  1:750914/1‑141 (MQ=255)
                                          aTTCTTATACTGGTATAATAGATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGaaa                                                                                                  >  2:888296/1‑140 (MQ=255)
                                           ttCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACg                                                                                                 <  2:873277/141‑1 (MQ=255)
                                           ttCTCAAAATGGAGTAGTAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACg                                                                                                 >  2:757674/1‑141 (MQ=255)
                                            tCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATCGAACGa                                                                                                >  1:711230/1‑141 (MQ=255)
                                                 aaCTGGTATAATAAATTTATGGTGAAAGGAATACTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATTAAGGTGTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATATAACGAATaaa                                                                                           <  1:469188/141‑1 (MQ=255)
                                                  aCTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCAGTTTTAGTCAAAGGTAACATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTCAACAAATGCCTGATAGAAAGAATaaaa                                                                                          >  2:627493/1‑140 (MQ=255)
                                                  aCTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAg                                                                                          <  2:569464/141‑1 (MQ=255)
                                                  aCTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAg                                                                                          >  1:659160/1‑141 (MQ=255)
                                                   cTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGa                                                                                         <  2:278138/141‑1 (MQ=255)
                                                      gTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAATGAATAAAGAAAt                                                                                      >  1:162563/1‑141 (MQ=255)
                                                      gTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAAt                                                                                      >  1:1063952/1‑141 (MQ=255)
                                                       tataATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAAtt                                                                                     <  1:536258/141‑1 (MQ=255)
                                                         taataaATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAAttca                                                                                   >  1:397385/1‑139 (MQ=255)
                                                         taataaATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTAATAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTaa                                                                                   >  2:60548/1‑141 (MQ=255)
                                                                tttATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGGACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGa                                                                            <  2:565609/141‑1 (MQ=255)
                                                                tttATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGGACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGa                                                                            <  2:1012487/141‑1 (MQ=255)
                                                                tttATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACCTCAATAAAGATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGa                                                                            >  1:771269/1‑141 (MQ=255)
                                                                 ttATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAAGAc                                                                           >  1:45642/1‑141 (MQ=255)
                                                                 ttATGTTGAAATGAAGATTATATGACAGGGTATTCACTTTTAGTAAAACGTAAAATTAAATAAACGTATTATCGTACGTATTTAAATATACGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGAc                                                                           >  1:792605/1‑141 (MQ=255)
                                                                  tATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACg                                                                          >  2:988122/1‑141 (MQ=255)
                                                                    tGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCt                                                                        >  1:893253/1‑141 (MQ=255)
                                                                     gTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCtt                                                                       >  1:391035/1‑141 (MQ=255)
                                                                     gTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCtt                                                                       <  1:63060/141‑1 (MQ=255)
                                                                      ttGAAATGAATATGGTATGATAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGTACGCttt                                                                      <  1:213362/141‑1 (MQ=255)
                                                                          aaTGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTg                                                                  <  1:806104/141‑1 (MQ=255)
                                                                           attaaTATTGTATGACAGGGTATTCACTTTAATTAAAAAGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATCGc                                                                 <  2:686056/138‑1 (MQ=255)
                                                                               atatTCGATGACAGGGTATTCCCTTGTGTAAAAAGGTAAACTTAAATAAAGGTTTTATAGAACGTACTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACt                                                             >  1:780751/1‑141 (MQ=255)
                                                                                  ttGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCa                                                          <  2:986825/141‑1 (MQ=255)
                                                                                       tGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAAt                                                     <  1:267630/141‑1 (MQ=255)
                                                                                        taCAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATCTGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTACATCAATc                                                    <  2:789499/140‑1 (MQ=255)
                                                                                        gACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATc                                                    <  1:356741/141‑1 (MQ=255)
                                                                                         aaaGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACTCTTTATTGCAACTGCATCAATCg                                                   <  2:227158/139‑1 (MQ=255)
                                                                                         aCAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCg                                                   <  2:1059366/141‑1 (MQ=255)
                                                                                         aCAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCg                                                   <  2:769107/141‑1 (MQ=255)
                                                                                         aCAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCg                                                   <  1:967575/141‑1 (MQ=255)
                                                                                           aGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTa                                                 >  1:1006812/1‑141 (MQ=255)
                                                                                            gggTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAAGATGCTATGAAATTAAAACAAGACGCATTATTGCAACTGCCTCAATCGtgt                                                >  1:1037791/1‑139 (MQ=255)
                                                                                              gTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAATAAATTAAAAAAGGACGCTTTATTGCAACTGCATCAATCGTATtc                                              >  1:873972/1‑141 (MQ=255)
                                                                                                  tCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCaa                                          <  1:475997/141‑1 (MQ=255)
                                                                                                   cACTGTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAAAATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAat                                         >  1:723592/1‑141 (MQ=255)
                                                                                                    aCTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTGTTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAata                                        >  2:567467/1‑141 (MQ=255)
                                                                                                     cTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAatat                                       <  2:313100/141‑1 (MQ=255)
                                                                                                      ttttATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAAtatt                                      >  1:1002565/1‑141 (MQ=255)
                                                                                                         tattaAAAGGTAAAAATAAATAAAGGTTTTATAGAACGTATTTTTTTTCAAATATATTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATattatt                                   <  1:1037666/141‑1 (MQ=255)
                                                                                                         aattaAAAAGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATattatt                                   <  1:266779/140‑1 (MQ=255)
                                                                                                          attaAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATATTATTg                                  >  2:534702/1‑141 (MQ=255)
                                                                                                          attaAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATATTATTg                                  >  1:602800/1‑141 (MQ=255)
                                                                                                             aaaaGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATATTATTGatt                               >  1:441551/1‑141 (MQ=255)
                                                                                                               aaGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATATTATTGattat                             >  2:408178/1‑141 (MQ=255)
                                                                                                                   taaatgtaaattaaGGGTTTTTAGAACGTATTTAAATATATGAGGAGTAAACAAATTGCTTAGAGAACGAATAAAGAAATTAAAACTTGTCGTGTTACTGCAACTGCATCAATCGTATTCTCAATATTATTGATTATTCAt                         <  2:272539/135‑1 (MQ=255)
                                                                                                                        ataaataaaGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGCCGCTTAATTTCAACTGCATCAATCGTATTCTCAATATTATTGATTATTCATTACtt                    <  1:411665/140‑1 (MQ=255)
                                                                                                                          aaataaaGGTTTTATAGAACGAATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGATTAATCGCAACTACATCAATCGTATTCTCAATATTATTGATTATTAATTACtttt                  >  2:341475/1‑140 (MQ=255)
                                                                                                                               aaGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATAGCAACTGCATCAATCGTATTCTCAATATTATTGATTATTCATTACTTTGTTTct             >  2:783187/1‑140 (MQ=255)
                                                                                                                                aGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATATTATTGATTATTCATTACTTTGTTTCGt            >  2:74241/1‑141 (MQ=255)
                                                                                                                                        taGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTCTTGCAACTGCATCAATCGTATTCTCAATATTATTGATTATTCATTACTTTGTTTCGTTGGATAAt    <  2:566182/141‑1 (MQ=255)
                                                                                                                                          gAACGTATTTAAAGATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGAACGCTTTATTGCAACTGCATCAATGGTATTCTCAATAGTATTGATTATTCATTACTTTGTTTCGTTGGGTAATgg  >  2:141533/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACTAATAATCATGAGGTAACTAATAACACATATTTAACTTGTATTCTTAAACTGGTATAATAAATTTATGTTGAAATGAATATTGTATGACAGGGTATTCACTTTTATTAAAAGGTAAAATTAAATAAAGGTTTTATAGAACGTATTTAAATATATGAGGAGTAAACAAATGGCTGATAGAACGAATAAAGAAATTAAAACAGGACGCTTTATTGCAACTGCATCAATCGTATTCTCAATATTATTGATTATTCATTACTTTGTTTCGTTGGATAATGC  >  CP000730/863642‑863920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: