Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,440,085 G→T 89.2% intergenic (‑45/‑891) cspA ← / → xpaC cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,440,0850GT89.2% 139.6 / 11.4 69intergenic (‑45/‑891)cspA/xpaCcold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base T (21/37);  total (24/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01

TGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAAT  >  CP000730/1439948‑1440219
                                                                                                                                         |                                                                                                                                      
tGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCat                                                                                                                                     <  1:860531/141‑1 (MQ=255)
    aaaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTa                                                                                                                                 <  2:13669/141‑1 (MQ=255)
     aaTATACGAATACATCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGCTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTAc                                                                                                                                <  1:88442/141‑1 (MQ=255)
       tGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAACATTCATTCAATATGCTTATTCTCATATTACaa                                                                                                                              >  1:177092/1‑141 (MQ=255)
        gTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTTCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACaaa                                                                                                                             >  2:225058/1‑141 (MQ=255)
               ttcGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATCTGCTTCTTCTTATATTACAATTAATACa                                                                                                                      <  2:96387/139‑1 (MQ=255)
                 cGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATg                                                                                                                    >  1:328704/1‑141 (MQ=255)
                       tttCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTGGTTTCATAATCTGAAACCTCCAAGACTAAATTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAAtt                                                                                                              <  2:266960/141‑1 (MQ=255)
                            ccTTCAACTTCAATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACt                                                                                                         <  2:676442/141‑1 (MQ=255)
                              ttCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTTTACCTTGTTTCATAATCTTAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCa                                                                                                       <  1:141486/141‑1 (MQ=255)
                                cAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAACAAATACATGCAAATTTCACTCaaa                                                                                                     <  2:47019/141‑1 (MQ=255)
                                cAACGTCGATAAAGCCGAATCCTTTGTCAGCGTTAAACCATTTATCTGTGCCGTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCgaa                                                                                                     <  2:447236/141‑1 (MQ=255)
                                 acctccGATACTGCCCAATCCTTTTTCAGCGTTAAACCATTTACCTGTACCTTGTCTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAACAAATACATGCAAATTTCACTCAAat                                                                                                    <  1:78907/136‑1 (MQ=255)
                                 aaCTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCCAat                                                                                                    <  2:933332/141‑1 (MQ=255)
                                 aaCTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCACTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAat                                                                                                    <  2:848863/141‑1 (MQ=255)
                                   cTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAatat                                                                                                  <  1:197319/141‑1 (MQ=255)
                                    ttCGAAAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTCCCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATAtt                                                                                                 <  1:972597/141‑1 (MQ=255)
                                       gATAAAGCCGAATCCTTTTTCAGCGTTCATCTTTTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                                                                                              >  1:1051179/1‑141 (MQ=255)
                                       gATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTAACTCAAATATTCtt                                                                                              >  2:550723/1‑141 (MQ=255)
                                       gATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCCCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                                                                                              >  1:763622/1‑141 (MQ=255)
                                             gCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATCTTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAat                                                                                        >  1:242999/1‑141 (MQ=255)
                                             gCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCaaa                                                                                        >  1:46985/1‑140 (MQ=255)
                                             gCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAat                                                                                        >  1:495726/1‑141 (MQ=255)
                                             gCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAat                                                                                        >  1:47131/1‑141 (MQ=255)
                                                  aTCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCGAAGACTAAAATTCATTCAATATGCTTATTCTCCTATTACAAAAAATACATGCAAATTTCCCTCAAATATTCTTTGCAATATGGa                                                                                   >  2:194683/1‑141 (MQ=255)
                                                  aTCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGa                                                                                   <  1:1033296/141‑1 (MQ=255)
                                                     cTTTTTCAGCGGTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATAGTCTTTGCAATATGGaata                                                                                <  2:879051/141‑1 (MQ=255)
                                                      ttgatCAGCGTCAAACCATTTAACTGTACCTTGCTGCATATTCTTAAACTTCCAACATGAAAATTTATTCAATATGCTTATTCTCATAGTACAAAAAATACATGCAAATTTCACTCAAAGATTCTTTGCAATATGGaataa                                                                               <  2:294994/137‑1 (MQ=255)
                                                      cttttCAGCGTTAAACCATTTAACTGTTCCTTGTTTCATAATCTGAAACCTCCAAGACTAAACTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGaataa                                                                               <  2:37003/140‑1 (MQ=255)
                                                       ttttCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACGAAAATTCATTCAATATGCTTAATACCATTTTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGaataat                                                                              <  1:201248/141‑1 (MQ=255)
                                                          tCAGCGTTAACCCATTTAACTGTACCGTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTTATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAatcat                                                                           <  2:923032/141‑1 (MQ=255)
                                                          tCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAatcat                                                                           >  1:998306/1‑141 (MQ=255)
                                                             gCGTTAAACCATTTAACTGTACCTTCTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTg                                                                        <  2:920521/141‑1 (MQ=255)
                                                                ttAAACCATTTAACTGTACCTTGTTTCATAATCTGAACCCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCTAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCtt                                                                     <  1:727374/141‑1 (MQ=255)
                                                                ttAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTCATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCtt                                                                     <  2:405172/141‑1 (MQ=255)
                                                                 aaacccATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTa                                                                    <  2:571568/137‑1 (MQ=255)
                                                                   aaCCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCTATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATCTTCTTTGCAATATGAAATAATCATCTGCTTAAc                                                                  >  2:163760/1‑141 (MQ=255)
                                                                   aaCCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAAc                                                                  >  2:163506/1‑141 (MQ=255)
                                                                    aCCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTAGTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACt                                                                 <  2:436289/141‑1 (MQ=255)
                                                                           aaCTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCCCTCTATTATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGtatta                                                          <  2:584877/141‑1 (MQ=255)
                                                                           aaCTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTAAAAAAAAAAAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGtatta                                                          <  2:1016441/141‑1 (MQ=255)
                                                                                      gTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGttt                                               >  2:64065/1‑140 (MQ=255)
                                                                                       tttCATAGTCCGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGttat                                              >  1:662083/1‑141 (MQ=255)
                                                                                        ttCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATAATCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGttatt                                             <  1:68267/141‑1 (MQ=255)
                                                                                        ttCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGttatt                                             <  2:1007090/141‑1 (MQ=255)
                                                                                             aaTCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTACACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTATATTATAGTGCAGTTATTACCaa                                        >  2:169129/1‑141 (MQ=255)
                                                                                               tCTGAAACCTCCAAGACTAAAATTCAATCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAAt                                      >  2:659424/1‑141 (MQ=255)
                                                                                                 tGAACCCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTg                                    <  2:221087/141‑1 (MQ=255)
                                                                                                  gAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGc                                   >  2:497280/1‑141 (MQ=255)
                                                                                                   aacccTCCAAGACTAAAAATCACTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGca                                  <  2:1040338/138‑1 (MQ=255)
                                                                                                       cTCCGAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTCTATTATAGTGCAGTTATTACCAAATTGcaatac                              >  1:720333/1‑141 (MQ=255)
                                                                                                       cTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTTAAATATTCTTTGCAATATGGAATAATCATCTGCTTAATTTGTATTGTAGTGCGGTTATTACCAATTTGgaatta                              >  2:810018/1‑139 (MQ=255)
                                                                                                         ccAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTACCTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGcaatacaa                            >  1:406700/1‑141 (MQ=255)
                                                                                                              aCTGAAATTCATGCAATATGCTTATAAGAATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAgtgata                       <  2:973309/141‑1 (MQ=255)
                                                                                                               cgtaaattccgtacatatGCTTATACTCATATTACAAAAAATACATGCAAATTTCATTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTGATTACCAAATTGCAATACAAtaataa                      <  2:731517/127‑1 (MQ=255)
                                                                                                               cTAAAATTCTTTTAACATGTGTATTCTCATATTACAAAAAATACATTCAAATTTCACTCAAATATTCTTTGCAATATGTAATAATTATCTGCTTAACTTGTATTGTAGTGCAGTTATTTCCAAATTGCAATACAAtaataa                      <  2:296087/141‑1 (MQ=255)
                                                                                                               cTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAAtaataa                      <  2:324555/141‑1 (MQ=255)
                                                                                                               cTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAACATCGAATACTCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAAtaataa                      <  2:850657/141‑1 (MQ=255)
                                                                                                                tAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATaaa                     <  2:244032/141‑1 (MQ=255)
                                                                                                                 aacctccactcaATACGCTTATTCACATCTTACAACAAAACCATCCAACTCTCACTCAACTACACTTCGCAATATGGAATAATCACCTGCCTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATaaaa                    <  1:480019/132‑1 (MQ=255)
                                                                                                                 aaacTTCATTCAATATGCTTATTCTCAAATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATaaaa                    <  1:981231/141‑1 (MQ=255)
                                                                                                                 aaaaTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATaaaa                    <  1:1030789/141‑1 (MQ=255)
                                                                                                                     ttcattcaATATGCTTATTATCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAata                <  1:982267/141‑1 (MQ=255)
                                                                                                                      tcattcaATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAatat               <  1:225058/141‑1 (MQ=255)
                                                                                                                         ttcaATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTCTTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCa            >  1:609847/1‑141 (MQ=255)
                                                                                                                         ttcaATATGCTTATTAGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGACATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCa            <  1:141355/141‑1 (MQ=255)
                                                                                                                             atatGCTTATTCTCATATTAAAAAAAATACATGCAACTTTCACTCACATATTCTTTGCAATATGTAATAATCATCTGCTTAACTTGTATTATAGTGTAGTTATTTCCAAATTGCAATACAATAATAAAACATATTCATTTa        >  1:262662/1‑141 (MQ=255)
                                                                                                                                  cTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCaa   <  1:429950/141‑1 (MQ=255)
                                                                                                                                   ttATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAat  <  2:328704/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAAT  >  CP000730/1439948‑1440219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: