BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,821,268	C→T	9.0%	M257I (ATG→ATA)	ald ←	alanine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,821,268	0	C	T	9.0%	276.9 / 3.1	103	M257I (ATG→ATA)	ald	alanine dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (48/44); new base T (9/2); total (57/46)
Fisher's exact test for biased strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GCACCTGGCATATTTGCAACTGCATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATAATTGTATGTACACGTCCACC  >  CP000730/1821128‑1821408

gCACCTGGCATATTTGCAACTGCATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTc                                                                                                                                              >  2:540798/1‑141 (MQ=255)

gCACCTGGCATATTTGCAACTGAATCATGCAACACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTc                                                                                                                                              >  1:253514/1‑141 (MQ=255)

   ccTGGTATATTTGCAATTCCATAATGAACCACACCATGCTTAATATATGGAGGATTATCATGTGTCGTCATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGTAATGTCAATAATAACTGACCCATTTTTCAtt                                                                                                                                           <  1:147594/141‑1 (MQ=255)

     tgcactgttttccaCTCCATAATGAACCACACCATGCTTAATATATTTAGGATCATCATCTGTCGTAATTTTATCAGTTGTTTCAACAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTg                                                                                                                                         <  1:343877/128‑1 (MQ=255)

         agatTTGCAACTGCATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTCTCAAACATACCTCCTGGAAAATTCAATTTTTCAATAATGACTGACCCATTTTCTATTTGTTTa                                                                                                                                     <  1:776709/139‑1 (MQ=255)

          tatTTGCAACTGCATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTat                                                                                                                                    >  1:897717/1‑140 (MQ=255)

            tttGCAACTGCATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATCGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATc                                                                                                                                  <  2:449587/141‑1 (MQ=255)

                cAACTGCATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGt                                                                                                                              >  1:824079/1‑141 (MQ=255)

                 aaCTGCATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGtt                                                                                                                             >  1:458010/1‑140 (MQ=255)

                      cATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTg                                                                                                                        >  1:210509/1‑141 (MQ=255)

                          aTGAGCCACCCCATGCTTAATTCATGTAGGCTCCTCATGTGTCGTAACTTCATCAGTTGTTTCGAAAAGACCGCCTTGATCAATAGCAATGTCAATTATTACTGGCCCATTTTTCATTTGTTTAATCATGTCTTCTGTTAc                                                                                                                    <  2:337407/141‑1 (MQ=255)

                           tGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCCATAATAACTGACCCATTTTTCATTTGGTTAATCATTTATTATGTTaaa                                                                                                                   >  2:91343/1‑139 (MQ=255)

                           tGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACa                                                                                                                   >  2:58418/1‑141 (MQ=255)

                           tGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTGGTTGAATAGCAATGTCAATAATAACTGACCCATTTTTTTTTTGTTTATTGATGTCTTATGATaaa                                                                                                                   >  2:639832/1‑139 (MQ=255)

                             aaCCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAg                                                                                                                 >  1:41652/1‑141 (MQ=255)

                              aCCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGt                                                                                                                <  2:187107/141‑1 (MQ=255)

                               ccACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCGTTCGCGAGTTCATGTCTTCTGTTACAAGTc                                                                                                               <  1:254600/141‑1 (MQ=255)

                                caaaacATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTAACAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCt                                                                                                              <  2:256285/136‑1 (MQ=255)

                                 acacCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTGGTTTCAAAAATACCGCCTTGACCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCtt                                                                                                             >  2:743057/1‑141 (MQ=255)

                                  cacCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCTTTTTTTTTATAATTTCTTCGGTTCCAAGTCTTg                                                                                                            >  2:570574/1‑141 (MQ=255)

                                  acccgttcatAATATATGGAGGATCATCATGTTTCGTAATGTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATATCTGACCCATTTTTTATTTGTTTAATCATGTCTTCTGTTACAAGTCTTg                                                                                                            <  2:504098/132‑1 (MQ=255)

                                     cATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTgacc                                                                                                         >  1:418579/1‑138 (MQ=255)

                                      aTGCTTAATTTTTGTAGGTTCATCATGTGTTCTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATCACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGgcgc                                                                                                        <  1:21945/141‑1 (MQ=255)

                                      aTGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGACTTTgcgc                                                                                                        >  1:898557/1‑141 (MQ=255)

                                       tGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCt                                                                                                       <  1:716539/141‑1 (MQ=255)

                                         cTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCttt                                                                                                     >  1:364728/1‑141 (MQ=255)

                                           tAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAg                                                                                                   <  1:334237/141‑1 (MQ=255)

                                              tatatGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCAc                                                                                                >  1:235893/1‑141 (MQ=255)

                                               atatGTATCATCATCATGTGTCGTCATTTTATCTGTTTTTTCAAAAATACCCCCTTGATCAATAGCAATGTCAATAATAACTGTCCCATTTTCCATTTGTTTAATCATGACTTCTGTTACAAGTCTTGGCGCTTTAGCAcc                                                                                               <  2:876913/141‑1 (MQ=255)

                                                 atGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTg                                                                                             <  1:540798/141‑1 (MQ=255)

                                                   gTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGTTTTAGCCCCTGGa                                                                                           >  2:424844/1‑141 (MQ=255)

                                                   gTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGa                                                                                           >  1:539888/1‑141 (MQ=255)

                                                    tAGGATCATCATGTGTCGTAGTTTTATCAGTTGTTTCAAAAATACCGGCTTGATCAATGGCAATGTCAAAAATAACTGACCGGTTTTTCATTGTTTGAATGATGTCTTCTGTTAGAAGTCTGAGCGCATTAGCAGCTGCaa                                                                                          >  2:274963/1‑141 (MQ=255)

                                                       gttcatcaTGTGTTGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTa                                                                                       <  1:78719/139‑1 (MQ=255)

                                                       gATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTa                                                                                       >  1:94599/1‑141 (MQ=255)

                                                       gATCATCATGTGTCGTAATTGTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTGACAAGTCTTGCGCCTTTACCACCCGGAATTa                                                                                       >  1:161406/1‑141 (MQ=255)

                                                         tcatcaTGGTTCGTAATTTTATCAGTTGTTTCAAAAAATCCGCCGTTATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTaaa                                                                                     <  2:480449/141‑1 (MQ=255)

                                                           atcatGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCGCCTGGAATTAAAAc                                                                                   <  2:1006861/141‑1 (MQ=255)

                                                           atcatGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAAc                                                                                   >  2:66228/1‑141 (MQ=255)

                                                             catGTGTCGTTATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTg                                                                                 <  1:758602/141‑1 (MQ=255)

                                                             catGTGTCGTAATTTTATCAGTTGTCTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTg                                                                                 >  1:531093/1‑141 (MQ=255)

                                                              atGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGc                                                                                >  1:533908/1‑141 (MQ=255)

                                                              atGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTCGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGc                                                                                <  1:649299/141‑1 (MQ=255)

                                                               tgtgTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCa                                                                               >  2:1025866/1‑141 (MQ=255)

                                                               tgtgTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCa                                                                               >  2:389767/1‑141 (MQ=255)

                                                               tgtgTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTCGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCa                                                                               <  2:290828/141‑1 (MQ=255)

                                                                gtgtCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCAc                                                                              >  2:366823/1‑141 (MQ=255)

                                                                  ttCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCt                                                                            <  2:825414/140‑1 (MQ=255)

                                                                      tAATTTTCTCAGTTGCTTCAAAAAGACCGCCTTGATCAATAGCAATGTCAATAATACCTGACCCATTTTTCATTTGTTTAATCATGGCTTCGGTTACAAGTCTTCGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTa                                                                        <  1:89009/141‑1 (MQ=255)

                                                                        aTTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTTTGTTACAAGTCTTGGCGCTTTAGCACCTGGATTTAAAACTGCACCTATTACt                                                                      >  2:570389/1‑141 (MQ=255)

                                                                         ttttATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTTTTTTTTTTAATTATGTCTTCTGTTCAAAGTCTTGGCGCTTTAGTGTATGGATTGCTAACTGCACCTAttctta                                                                     >  2:476577/1‑137 (MQ=255)

                                                                         ttttATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTTATTTTTTTTATCATGTATTCTGTTACAAGTCTTGGCGTTTTTGCACCTGGAATTAAAACTGCAGCTATTCCTa                                                                     >  2:854928/1‑141 (MQ=255)

                                                                         ttttATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCTTTTTTTTTATCATGTCTTCTGTTACAAGTCTTTGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTa                                                                     >  2:130527/1‑141 (MQ=255)

                                                                          tttATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTaa                                                                    <  2:793925/141‑1 (MQ=255)

                                                                            tATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAGTGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTCAAt                                                                  >  1:592160/1‑141 (MQ=255)

                                                                            tATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAATTCTTGGCGCTTTAGTACCTGGAATTAAAACTGCACTTATTCCTAAAt                                                                  >  2:496759/1‑141 (MQ=255)

                                                                              tCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTTATTTTTTTTTTCATGTGTTCTGTAACAAGACTTGGCGCTTTGTAACCTGGAGTTGAAACTGGACCTTTTACTAAAtaa                                                                >  2:692484/1‑139 (MQ=255)

                                                                                   tgttTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCGCCTATTACTAAATCACTTTg                                                           <  1:645238/141‑1 (MQ=255)

                                                                                   tcttttAAAAATACCGCCTTGATCAATAGCAATGTCAATAATTACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTg                                                           <  1:565806/139‑1 (MQ=255)

                                                                                    ttttCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCCTGGCGCTTTATCTCCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGt                                                          <  2:851584/140‑1 (MQ=255)

                                                                                      ttCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGttt                                                        >  1:40800/1‑141 (MQ=255)

                                                                                       tCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCCCCTATTACTAAATCACTTTTTTTa                                                       >  2:145063/1‑141 (MQ=255)

                                                                                         aaaaaTACCGCCTTGATCAATAGCAATGTCAATAATAACTGGCCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTCGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAAc                                                     <  1:952288/141‑1 (MQ=255)

                                                                                         aaaaaTACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAAc                                                     <  1:177149/141‑1 (MQ=255)

                                                                                         aaaaaTACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAAc                                                     <  2:469601/141‑1 (MQ=255)

                                                                                          aaaaTACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACa                                                    <  2:76046/141‑1 (MQ=255)

                                                                                           aaaTACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTTTTTTGTTTTTTAATGTCTTTTGTTACAAGTCTTGGCGCTTTTGTACCTGGAATTCAAACTGTGCCTTTTACTAAATTCCTTTTTTAAAAAt                                                   >  2:384561/1‑141 (MQ=255)

                                                                                             aTACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTTTTTTTTTTTTTCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATAc                                                 >  2:472101/1‑141 (MQ=255)

                                                                                             aTACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATAc                                                 >  1:941686/1‑141 (MQ=255)

                                                                                             aTACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATAc                                                 >  1:107579/1‑141 (MQ=255)

                                                                                             aTACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTGTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATAc                                                 >  2:331515/1‑141 (MQ=255)

                                                                                                ccGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAAc                                              >  1:444621/1‑141 (MQ=255)

                                                                                                ccGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTCTAACATACAAc                                              >  1:727418/1‑141 (MQ=255)

                                                                                                  gCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTc                                            <  2:627896/141‑1 (MQ=255)

                                                                                                   ccTTGATCAATAGCACTGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCa                                           <  1:663552/141‑1 (MQ=255)

                                                                                                   ccTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCa                                           >  1:445720/1‑141 (MQ=255)

                                                                                                      tGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTCTTACTAAATCACTTTGTTTAACATACACCTCAatt                                        >  1:1004801/1‑140 (MQ=255)

                                                                                                      tGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAata                                        >  1:193394/1‑141 (MQ=255)

                                                                                                      tGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAACCATGTCTTCTGTTACTTGTCTTGGCGCTTTAGCACCTGGAATTAAAACTCCACCTATTACTAAATCACTTTGTTTAACGTACAACTCAata                                        >  1:1004796/1‑141 (MQ=255)

                                                                                                           aaTAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCaa                                   <  2:864774/141‑1 (MQ=255)

                                                                                                            aTAGCAATGTCAATAATAACTGACCCATTTTTTTTTTTTTTTTTCATGTCTTCTGTTACAAGTCTTTGCGCGTTAGCACTTGGGATTAAGAGTGGACCTATTACTGAATCCCTTTGTTTAACATACAACTCAATATTCAAc                                  >  2:793528/1‑141 (MQ=255)

                                                                                                            aTAGCAATGTCAATAATAACTGAACCATTTTTCATTTGTTTAATCATGTCTTCTGTTGCAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCGCCTATTACTAAATCACTTTGTTTCACATACAAATCAATATTCAAc                                  >  1:1019/1‑141 (MQ=255)

                                                                                                             tAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCCCCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACg                                 <  2:253514/141‑1 (MQ=255)

                                                                                                               gtaagttCAATAATAACTTACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGa                               <  2:399786/135‑1 (MQ=255)

                                                                                                                  aTGTCAATAATAACTGACCCATTTTTTTTTTGTTTTTTCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAACTCACTTTGTTTAACATACAACTCAATATTCAACGGAttt                            >  2:184498/1‑141 (MQ=255)

                                                                                                                  aTGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGAttt                            <  1:549052/141‑1 (MQ=255)

                                                                                                                    ggcAAGAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGa                          <  1:1033035/139‑1 (MQ=255)

                                                                                                                    gTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGa                          >  1:613718/1‑141 (MQ=255)

                                                                                                                    gTCAATAATAACTGACCCATTTTTCATGTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGa                          <  1:277224/141‑1 (MQ=255)

                                                                                                                     tCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGAc                         >  1:307964/1‑141 (MQ=255)

                                                                                                                     tCAAGAATAACTGACCCATTTTTTTTTTTTTTTTTCATGTCTTCTGTTACAAGTCTTGACGCTTTGGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTATTTAACATACAAATAAATATTCAACGGATTTgtt                         >  2:733845/1‑139 (MQ=255)

                                                                                                                      cAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACAGATTTGACa                        >  1:511046/1‑141 (MQ=255)

                                                                                                                         tcatagcgggcccATTTTTCATTGGTTTAATCATGGCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATaa                     <  2:898557/131‑1 (MQ=255)

                                                                                                                                     cATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATAATTGTATGTacac         >  1:1012657/1‑141 (MQ=255)

                                                                                                                                     cATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATAATTGTATGTacac         <  1:495538/141‑1 (MQ=255)

                                                                                                                                     cATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATAATTGTATGTacac         <  2:210509/141‑1 (MQ=255)

                                                                                                                                     cATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATAATTGTATGTacac         <  1:635390/141‑1 (MQ=255)

                                                                                                                                       tttttCATTTTTTTAATCATGTCTTCTGTTACAAGTCTTGGCTCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAATTCACTTTGTTTAACATACAACTCATTATTCCACGGATTTGACATAAATGTATTTacactt       >  2:324119/1‑139 (MQ=255)

                                                                                                                                       tttttCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATAATTGTATGTACACGt       <  2:458010/141‑1 (MQ=255)

                                                                                                                                            cATTTGTTTAATCATGTCTTCTGTTACAAGGCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATAATTGTATGTACACGTCCAcc  <  1:991419/141‑1 (MQ=255)

GCACCTGGCATATTTGCAACTGCATAATGAACCACACCATGCTTAATATATGTAGGATCATCATGTGTCGTAATTTTATCAGTTGTTTCAAAAATACCGCCTTGATCAATAGCAATGTCAATAATAACTGACCCATTTTTCATTTGTTTAATCATGTCTTCTGTTACAAGTCTTGGCGCTTTAGCACCTGGAATTAAAACTGCACCTATTACTAAATCACTTTGTTTAACATACAACTCAATATTCAACGGATTTGACATAATTGTATGTACACGTCCACC  >  CP000730/1821128‑1821408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`