BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,928	T→A	46.0%	intergenic (‑1011/‑157)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,928	0	T	A	46.0%	62.2 / 198.9	156	intergenic (‑1011/‑157)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (46/38); new base A (39/33); total (85/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

TGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATT  >  CP000730/1929788‑1930068

tGTATATTTGCCGAGGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTTAATTAAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                                                                                                     <  1:933634/141‑1 (MQ=255)

tGTATATTCGCCGATGATATAATATTAGAGTCGCCTATCCCTCAGGCGTAAATCTAGACACAGAGAGAAGATTAA‑AAA‑G‑G‑CGA‑TGCTGAGTTTCGTTCACATCATAGCACCAGTCATCAGTGTCTGTGCCATTGCGttttt                                                                                                                                                     <  2:640740/141‑1 (MQ=255)

    tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                                                                                                >  1:894127/9‑141 (MQ=255)

     attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTGATCAGTGGCTGTGCTGTTGCGTATTATACttt                                                                                                                                               >  2:513247/8‑140 (MQ=255)

        tGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGACGCCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTACGTATTTCTCTTAtt                                                                                                                                             >  2:175705/1‑141 (MQ=255)

         acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTGTACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                           <  2:994198/138‑1 (MQ=255)

         acagatgggataataAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                           <  2:826198/132‑1 (MQ=255)

           cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                                                                                          >  1:1050144/1‑141 (MQ=255)

           agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGCTTCA‑TTACT‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                                         <  1:1040905/140‑1 (MQ=255)

            gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                                                                                                                         >  1:542007/1‑141 (MQ=255)

              tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                                      <  1:175163/138‑4 (MQ=255)

              tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                                      >  2:930199/4‑138 (MQ=255)

              tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑G‑T‑TGC‑TAATCATTTTCGTTCACATTATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                                                                                      >  2:583068/4‑141 (MQ=255)

              gggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                                      <  1:804605/138‑4 (MQ=255)

               ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     <  2:156225/139‑1 (MQ=255)

               ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     <  1:655840/139‑1 (MQ=255)

               ggtataAGAATCGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     <  2:33520/139‑1 (MQ=255)

               ggtataAAAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                                     <  2:614834/139‑1 (MQ=255)

                gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGACTTAGTa                                                                                                                                    >  1:59171/2‑141 (MQ=255)

                gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                    >  2:18345/2‑141 (MQ=255)

                gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                    <  2:539936/140‑1 (MQ=255)

                gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGAAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                                    <  1:200143/140‑1 (MQ=255)

                agataataGTAGAGTCGCCTATCTCGCAGGCGACAATTTAGACGCAGAGAGGAGGGGTA‑TTA‑G‑G‑TGA‑TGCTTATTTTCGGTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                                                                                                     <  1:395164/139‑1 (MQ=255)

                 tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                                   >  1:337062/1‑140 (MQ=255)

                  ataataataGGGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                                  <  2:838248/141‑3 (MQ=255)

                  ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                                  >  2:144909/1‑139 (MQ=255)

                  ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                                  <  2:911961/141‑3 (MQ=255)

                   aaataatAGAGCCGCCTATCGCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                                                                                                 <  2:873598/140‑4 (MQ=255)

                     ataagagagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                               <  2:178958/136‑1 (MQ=255)

                        atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑ac                                                                                                                          <  1:955160/140‑2 (MQ=255)

                        atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑aa                                                                                                                          >  2:475339/2‑140 (MQ=255)

                         tagaggcctcTATCTCTCAGGTGTCAATT‑‑GACGAAGTGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTGCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTCTTGGCTTAGTAAACGCa‑‑aca                                                                                                                         <  1:746053/132‑3 (MQ=255)

                         tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAGGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTCTTGGCTTAGTAAACGCa‑‑aca                                                                                                                         >  2:566309/1‑139 (MQ=255)

                         tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑aca                                                                                                                         >  2:737965/1‑139 (MQ=255)

                          agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                                        >  2:810943/1‑138 (MQ=255)

                          aaagTCGCCTATCTCTCAGGCAGCAA‑‑TGGACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGGCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                                        <  2:32700/139‑4 (MQ=255)

                            agTCGCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGC‑TAATCATTTTCGTTCACATCGTAGCACCAGGCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCA‑‑Acacat                                                                                                                      >  1:610664/1‑140 (MQ=255)

                             gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGGGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCTGTTGCGTATGATACTTATTGGCTTAGTAAAAGCa‑‑acaaata                                                                                                                     >  2:970926/1‑135 (MQ=255)

                              tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataa                                                                                                                    <  1:901024/141‑8 (MQ=255)

                               cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTCAGTAGACGCA‑‑ATACaa‑‑aa                                                                                                                  >  2:974809/1‑141 (MQ=255)

                               cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACaa‑‑aa                                                                                                                  >  1:138422/1‑141 (MQ=255)

                                gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AAt                                                                                                                 >  1:598935/1‑141 (MQ=255)

                                gCCTATCTCTCAGGCGTCAAAT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAGTCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATCATACTTATTGGCTTAGTACACGCa‑‑acaaataatt                                                                                                                  >  1:645268/1‑132 (MQ=255)

                                 ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCCCTTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACaa‑‑aaga                                                                                                                >  1:830701/1‑139 (MQ=255)

                                 ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATa                                                                                                                >  1:767668/1‑141 (MQ=255)

                                  cTATCTCTCAGGCGTCAATT‑‑GGCGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                >  1:551886/1‑130 (MQ=255)

                                  cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGCGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATCGCACCAGTCCTCAGTGGCTGTGCTGTTTCGTATTAGACTTATTGGCTTAGTAAACGCacgaagtaattat                                                                                                                  >  2:298574/1‑129 (MQ=255)

                                  cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                >  1:699709/1‑130 (MQ=255)

                                  cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                                >  1:975657/1‑130 (MQ=255)

                                   tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TTACTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGGATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaaa                                                                                                               >  1:438285/1‑129 (MQ=255)

                                    aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGt                                                                                                             >  2:846315/1‑141 (MQ=255)

                                    aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGt                                                                                                             >  1:1023345/1‑141 (MQ=255)

                                    aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGACATTGCGTTTTTTTCTTATTGGTTAAGTAGACGCA‑‑CTATAA‑‑AATAggg                                                                                                             >  2:958987/1‑140 (MQ=255)

                                    aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                              >  2:695494/1‑128 (MQ=255)

                                    aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                              <  2:256161/141‑14 (MQ=255)

                                     tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTTTTGTCTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaagag                                                                                                             <  2:437983/141‑15 (MQ=255)

                                     tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaatag                                                                                                             <  2:949714/141‑15 (MQ=255)

                                      ttctgAGGCTTCAAGTTAGACGCAGAGAGGAGGGGTA‑GAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGa                                                                                                           <  1:441641/140‑1 (MQ=255)

                                      ctttcAGGCGTCAATTTAGACGCAGAGAGTAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGa                                                                                                           <  1:286633/138‑1 (MQ=255)

                                       tctcAGGCGTCAATTTACACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGCGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAc                                                                                                          <  2:709455/141‑1 (MQ=255)

                                          cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACa‑ta                                                                                                      <  1:399780/141‑1 (MQ=255)

                                           aGGCGTCAATTTAGACGCGGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑CTACAA‑‑AATAGGTGAAa‑tat                                                                                                     >  1:210056/1‑141 (MQ=255)

                                           aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATCCAA‑‑AAAAGGTGCCa‑tat                                                                                                     >  1:76182/1‑141 (MQ=255)

                                           aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGAAGCA‑‑ATACAA‑‑AATAGGTGACa‑tat                                                                                                     >  1:135758/1‑141 (MQ=255)

                                                  aaTTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCa                                                                                            >  2:925655/1‑141 (MQ=255)

                                                    tttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑CTAGAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCAcc                                                                                          >  1:487394/1‑141 (MQ=255)

                                                       aGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAAt                                                                                       <  1:794324/141‑1 (MQ=255)

                                                           gCAGGGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCTCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATaaaa                                                                                   <  1:345224/141‑1 (MQ=255)

                                                                gaggagGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATccc                                                                              <  1:699398/141‑1 (MQ=255)

                                                                gaggagGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATccc                                                                              <  1:226373/141‑1 (MQ=255)

                                                                     ggTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCAc                                                                         <  1:23388/141‑1 (MQ=255)

                                                                     ggTGTA‑TAA‑G‑G‑TGA‑TGCTTACTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCAc                                                                         <  2:308173/141‑1 (MQ=255)

                                                                        gtA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTAc                                                                      >  2:735290/1‑141 (MQ=255)

                                                                             aa‑G‑G‑TGG‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCa                                                                  >  2:350452/1‑141 (MQ=255)

                                                                             aa‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCa                                                                  <  2:1030728/141‑1 (MQ=255)

                                                                              a‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCCTTGCGTTTTTTTGTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCaa                                                                 >  2:908138/1‑141 (MQ=255)

                                                                                g‑g‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCaaa                                                                <  2:937701/141‑1 (MQ=255)

                                                                                    ggA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATa                                                              <  2:346207/140‑1 (MQ=255)

                                                                                      gatgttgtttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAg                                                             <  1:496544/133‑1 (MQ=255)

                                                                                      a‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGt                                                            <  2:407981/141‑1 (MQ=255)

                                                                                          cgtATGTTCTTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCCTTTTTTTCTTATTGGCCAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCCCTCTACCGCAAATAGTGAg                                                         <  2:724028/139‑1 (MQ=255)

                                                                                           ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTTTTATTTTCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATACAAATCCCCTCACTACCGCAAATAGTGAgg                                                        >  2:11435/1‑141 (MQ=255)

                                                                                           ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        <  2:188102/141‑1 (MQ=255)

                                                                                           ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        <  1:511082/141‑1 (MQ=255)

                                                                                           ttATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTGTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgg                                                        >  1:1007127/1‑141 (MQ=255)

                                                                                            ttttttCGTTCCCATCATAGCACCAGTCATCAGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                       <  1:175705/139‑1 (MQ=255)

                                                                                            tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAGTGCGTTTTTTTGTTATTGGCTAAGTAGCCGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCCCTACCGCAAATAGTGAggg                                                       >  1:407725/1‑141 (MQ=255)

                                                                                             aTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                                      >  1:825140/1‑141 (MQ=255)

                                                                                             aTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                                      >  2:764792/1‑141 (MQ=255)

                                                                                              ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                                     <  2:44583/141‑1 (MQ=255)

                                                                                                  cGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTgg                                                 >  2:183628/1‑141 (MQ=255)

                                                                                                       acaTCATAGCACCAGTCATTAGTGCCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGtat                                            <  1:858724/141‑1 (MQ=255)

                                                                                                            atAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGGTGcat                                            >  1:415073/1‑141 (MQ=255)

                                                                                                            atAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGTGGTATAAGTa                                       >  2:243478/1‑141 (MQ=255)

                                                                                                               gCACCAGTCATCAGCGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGAACTTAGTGGGGGGATGGGTGTATAAGTAAAt                                    >  1:20602/1‑141 (MQ=255)

                                                                                                                       aatcgttgccTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                            <  1:392388/132‑1 (MQ=255)

                                                                                                                           aGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGCCG‑TGT‑ATCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGt                        <  1:1042748/141‑1 (MQ=255)

                                                                                                                             tGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTg                      <  2:1050144/141‑1 (MQ=255)

                                                                                                                                          gCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGAC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACt         >  2:297797/1‑141 (MQ=255)

                                                                                                                                                 tttCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAGTTTCGTTGTCTTAATTATACTGCTAAtt  >  1:599268/1‑141 (MQ=255)

TGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGACA‑TAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGCTAATT  >  CP000730/1929788‑1930068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`