Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,440,085	G→T	intergenic (‑45/‑891)	cspA ← / → xpaC	cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,440,085	0	G	T	100.0%	108.5 / NA	35	intergenic (‑45/‑891)	cspA/xpaC	cold shock protein CspA/5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/19);  total (16/19)

AAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAATTA  >  CP000730/1439952‑1440221                                                                                                                                       |                                                                                                                                         aaaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTa                                                                                                                                   <  1:589726/141‑1 (MQ=255)  aaTGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTAc                                                                                                                                  <  1:190569/141‑1 (MQ=255)     gTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCCTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACaaa                                                                                                                               >  1:609256/1‑141 (MQ=255)      tACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACaaaa                                                                                                                              <  1:68886/141‑1 (MQ=255)            ttcGTCATTTTCTCCTTCAACTTCGGTAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTCCCTTGTTTCATAATCTGAATCCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACa                                                                                                                        <  1:117329/139‑1 (MQ=255)              cGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATg                                                                                                                      >  1:301627/1‑141 (MQ=255)                    tttCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAAtt                                                                                                                <  1:585314/141‑1 (MQ=255)                        tcCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCAc                                                                                                            >  1:40022/1‑141 (MQ=255)                               aCTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAata                                                                                                     <  1:793139/141‑1 (MQ=255)                                    gATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                                                                                                <  1:821063/141‑1 (MQ=255)                                    gATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                                                                                                <  1:821053/141‑1 (MQ=255)                                    gATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCtt                                                                                                <  1:608472/141‑1 (MQ=255)                                                 ccTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTCAAATTCATTCAATATGCTTATTCTCATATTACAACAAATACATCCAAATTTCACTCCAATATTCTTTGCAATATGGcat                                                                                   >  1:737441/1‑141 (MQ=255)                                                   tttttCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGaataa                                                                                 >  1:319755/1‑141 (MQ=255)                                                      ctCACCGTTAAACCATTTACAATG‑ACCATGTTGCATAATCTGAAACCTTCAAGACTAATATTCATTCAATATGCTTATTCTCCTATTACAAAAAATACATGCAACTTTCACTCAAATATTCTTTGCAATATGGAATAatca                                                                              <  1:860603/140‑1 (MQ=255)                                                         aGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCACGACTAAAGTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCt                                                                           <  1:684830/141‑1 (MQ=255)                                                            gTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAGTATTCTTTGCAATATGGAATAATCATCGGCt                                                                        >  1:62945/1‑141 (MQ=255)                                                                          aCTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGtattat                                                           >  1:42985/1‑141 (MQ=255)                                                                              tACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCAATCAAATATTCTTTGCAATATGGAATAATCATCTGCTTACCTTGTATTAGAGTg                                                       >  1:816694/1‑141 (MQ=255)                                                                                     tttCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTGAACTTGTATTATAGTGCAGttat                                                >  1:339408/1‑141 (MQ=255)                                                                                         aTAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTAcc                                            <  1:94413/141‑1 (MQ=255)                                                                                                            aCTAAAATTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAataata                         >  1:190455/1‑141 (MQ=255)                                                                                                               aaaaTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATGTGCATTGATCTTGGGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAGTAATaaaa                      >  1:214323/1‑141 (MQ=255)                                                                                                               aaaaTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATaaaa                      >  1:899519/1‑141 (MQ=255)                                                                                                                 aaTTCATTCAATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATaaaaaa                    <  1:247071/141‑1 (MQ=255)                                                                                                                      attcaATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTc               <  1:781423/141‑1 (MQ=255)                                                                                                                         caATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATCCAATAATAAAAAATATTCGtt            >  1:354613/1‑141 (MQ=255)                                                                                                                         caATATGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCAtt            <  1:140656/141‑1 (MQ=255)                                                                                                                            tatGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTaa         >  1:76161/1‑141 (MQ=255)                                                                                                                            tatGCTTATTCTCATATTACAAAAAATACATGCAAATTTCACTAAAATATTCTTTGCAATATGGAATAATCATCTGATTAACTTGTATTATAGTGCAGTTATTAACAAATTGCAATACAATAATAAAAAATATTCATTTaa         >  1:173481/1‑141 (MQ=255)                                                                                                                                  tATTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAatt   <  1:304889/141‑1 (MQ=255)                                                                                                                                   tttCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAatta  <  1:573734/140‑1 (MQ=255)                                                                                                                                   aTTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAatta  >  1:876098/1‑141 (MQ=255)                                                                                                                                   aTTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAatta  <  1:574956/141‑1 (MQ=255)                                                                                                                                   aTTCTCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAatta  <  1:292816/141‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         AAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTA‑ACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAATAATCATCTGCTTAACTTGTATTATAGTGCAGTTATTACCAAATTGCAATACAATAATAAAAAATATTCATTTAATCAATTA  >  CP000730/1439952‑1440221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑