Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000730 1,929,861 T→C intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,929,8610TC84.1% 393.1 / 47.8 158intergenic (‑944/‑224)USA300HOU_1794/USA300HOU_1796hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/14);  new base C (69/63);  total (81/77)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

ATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACT  >  CP000730/1929721‑1929998
                                                                                                                                            |                                                                                                                                              
aTTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGgtgt                                                                                                                                                >  1:689053/1‑141 (MQ=255)
 ttAAGTGTAGACGATACAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGATTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGta                                                                                                                                               >  1:691977/1‑141 (MQ=255)
  tAAGTGTAGACGATTCAATCTATCAGTTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTTGCCTATCTCTCAGGCGTCAATTTGGACGCAGAGAGGAGGTGta‑t                                                                                                                                             <  1:563730/141‑1 (MQ=255)
              aTTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCtt                                                                                                                              <  1:501029/141‑1 (MQ=255)
                 cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGCTGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGAGGattatt                                                                                                                           >  1:31649/1‑141 (MQ=255)
                   aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTAtttt                                                                                                                         >  1:395577/1‑141 (MQ=255)
                    tCTATCAGCTAGAATCATATAACTCATCAGAAGCAAGTGCCTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTc                                                                                                                        <  1:156611/141‑1 (MQ=255)
                               gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCAcatcat                                                                                                             <  1:311445/141‑1 (MQ=255)
                                                 atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                          >  1:784066/15‑141 (MQ=255)
                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                         >  1:8360/14‑141 (MQ=255)
                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                         >  1:223499/14‑141 (MQ=255)
                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGGTCACATCATAGCACCAGTCAACAGTGGCt                                                                                         >  1:583249/14‑141 (MQ=255)
                                                  tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGAATTAC‑T‑T‑TGCTAATCATTTTCGTTTACATCATAGAACCAGTAATCAGTAGCt                                                                                         >  1:611557/14‑141 (MQ=255)
                                                   cggaagtagatatgCTTGTACAGTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                        <  1:615073/129‑1 (MQ=255)
                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGTGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  1:127338/130‑1 (MQ=255)
                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       >  1:853967/12‑141 (MQ=255)
                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  1:377961/130‑1 (MQ=255)
                                                    ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                       <  1:752439/130‑1 (MQ=255)
                                                     gaagtagatattcTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                      <  1:858467/129‑1 (MQ=255)
                                                     gaagtagatatgCTTGTATATTCCCAGTTGGTATAATAATAGCGACGCCGGATGCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                      <  1:436838/131‑1 (MQ=255)
                                                     gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                      <  1:748887/131‑1 (MQ=255)
                                                      aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                     <  1:533139/132‑1 (MQ=255)
                                                      aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                     <  1:234519/132‑1 (MQ=255)
                                                       agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                    <  1:196085/133‑2 (MQ=255)
                                                       agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                    <  1:781268/133‑2 (MQ=255)
                                                       agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                    <  1:330582/133‑2 (MQ=255)
                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                   <  1:116348/134‑3 (MQ=255)
                                                        gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAGTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                   >  1:553184/8‑139 (MQ=255)
                                                         tagatatgCTTGTATATTCACCGATGGTATAATAATCGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTGCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                  <  1:4528/135‑4 (MQ=255)
                                                         tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                  <  1:789671/135‑4 (MQ=255)
                                                         tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCTTCAGTGGCTGTGctgt                                                                                  <  1:460774/135‑4 (MQ=255)
                                                         tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                                   <  1:423954/141‑1 (MQ=255)
                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                <  1:747086/137‑6 (MQ=255)
                                                           gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                >  1:775013/5‑136 (MQ=255)
                                                            ggatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                               <  1:292530/138‑7 (MQ=255)
                                                             tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGc                                                                              <  1:367868/141‑1 (MQ=255)
                                                             tatgCTTGTATATTCACAGATGGTATGATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCGTCAGTGGCTGTGctgttgcg                                                                              <  1:561547/139‑8 (MQ=255)
                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCg                                                                             >  1:84840/3‑141 (MQ=255)
                                                             tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgcg                                                                              <  1:453797/139‑8 (MQ=255)
                                                                 cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGtatt                                                                         >  1:486822/1‑138 (MQ=255)
                                                                   tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGtattat                                                                       <  1:927463/141‑6 (MQ=255)
                                                                   tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGtattat                                                                       <  1:49769/141‑6 (MQ=255)
                                                                   tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGtattat                                                                       <  1:269028/141‑6 (MQ=255)
                                                                    gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGtattata                                                                      >  1:808288/12‑138 (MQ=255)
                                                                    gTATATTTGCCGATGCTATAATAGTAGAGTCGCCTATCACTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGtttttt                                                                       >  1:690330/1‑141 (MQ=255)
                                                                    gTATATTTGCCGATGCTATAATAGGAGAGTCGCCTATCTCGCAGGCGGCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTGCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGtttttt                                                                       <  1:28385/141‑1 (MQ=255)
                                                                     tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGGTGCATTAC‑T‑T‑TGCTAATCAATTTCGTTCACATCATAGCACCAGTCCTCAGTGGTTGTGCTGT‑TGCGtattatac                                                                     <  1:600291/141‑8 (MQ=255)
                                                                     tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGtattatac                                                                     <  1:390988/141‑8 (MQ=255)
                                                                          tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGC‑TGCGTATTATACTTAtt                                                                >  1:118684/6‑141 (MQ=255)
                                                                           cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTg                                                               >  1:340588/5‑141 (MQ=255)
                                                                            acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTgg                                                              <  1:272184/138‑1 (MQ=255)
                                                                             cagatggtATAATAATAGAGTCGCGTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGc                                                             >  1:676862/3‑141 (MQ=255)
                                                                             cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGc                                                             >  1:851500/3‑141 (MQ=255)
                                                                               gatgatATAAGAGTAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCt                                                            <  1:710638/141‑1 (MQ=255)
                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCttag                                                         <  1:652955/138‑4 (MQ=255)
                                                                                 tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAg                                                         >  1:890406/4‑141 (MQ=255)
                                                                                 tggtataATAATAGAGTCGCCTACCTCTTAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCttag                                                         <  1:353494/138‑4 (MQ=255)
                                                                                  ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGt                                                        <  1:9032/139‑1 (MQ=255)
                                                                                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTa                                                       >  1:903382/2‑141 (MQ=255)
                                                                                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTa                                                       >  1:522451/2‑141 (MQ=255)
                                                                                   gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTAGTTAAGAACTTAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTa                                                       >  1:674037/2‑141 (MQ=255)
                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTaaa                                                     <  1:726146/141‑3 (MQ=255)
                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTaaa                                                     >  1:218836/1‑139 (MQ=255)
                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTaaa                                                     >  1:409396/1‑139 (MQ=255)
                                                                                     ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCCTCAGTGCCCGTGCTGT‑TGCGTATTATACTTATTGGCttactaaa                                                     >  1:253075/1‑134 (MQ=255)
                                                                                      taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTaaac                                                    <  1:542973/141‑4 (MQ=255)
                                                                                      taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTaaac                                                    <  1:92418/141‑4 (MQ=255)
                                                                                       aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATTCTTATTGGCTTAGTAAACg                                                   <  1:509398/141‑1 (MQ=255)
                                                                                       aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACg                                                   <  1:212055/141‑1 (MQ=255)
                                                                                        ataatagAGTCGCCTATCTCTCAGGCTTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGc                                                  <  1:568654/137‑1 (MQ=255)
                                                                                             agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTGATCGGTGGCTGTGCTGT‑TGCGTAATATACTTATTGGCTTAGTAAACGGaacca                                             >  1:102543/1‑138 (MQ=255)
                                                                                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                            <  1:468046/141‑5 (MQ=255)
                                                                                              gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATACGAGGATCT‑CGATGTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                            >  1:628115/1‑137 (MQ=255)
                                                                                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                           <  1:259167/141‑6 (MQ=255)
                                                                                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                           <  1:921946/141‑6 (MQ=255)
                                                                                               agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAA‑T‑T‑TGCTAATCCTTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                           >  1:183951/1‑136 (MQ=255)
                                                                                               agTCGCCTATCTCTCAGGCGACAATTTACACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGCTGCTTATTTTCGTTCACATCATAGAACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                            >  1:599856/1‑141 (MQ=255)
                                                                                                gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                          >  1:744152/1‑135 (MQ=255)
                                                                                                 tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataa                                         >  1:306349/1‑134 (MQ=255)
                                                                                                  cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                        >  1:796012/1‑133 (MQ=255)
                                                                                                   gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                       >  1:908762/1‑132 (MQ=255)
                                                                                                    ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                      >  1:333302/1‑131 (MQ=255)
                                                                                                     cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                     >  1:867149/1‑130 (MQ=255)
                                                                                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                    >  1:385516/1‑129 (MQ=255)
                                                                                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTCGTAGACTCaacagatgattaat                                    >  1:321202/1‑129 (MQ=255)
                                                                                                      tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAAGTGCATTAC‑T‑T‑TGCTAATCATTTTAGTTCACATCATAGCACCAGTCACCCGTGGCTATGTTGT‑GGCGTATTTTACTTATTGGCTTAGTCAACGCaacaaataattaat                                    >  1:210941/1‑129 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTCGTAAACCCaacaaataattaata                                   >  1:234487/1‑128 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                   >  1:245740/1‑128 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                   >  1:767529/1‑128 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                   >  1:606846/1‑128 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                   <  1:667681/141‑14 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                   >  1:740294/1‑128 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                   >  1:747941/1‑128 (MQ=255)
                                                                                                       aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                   >  1:731859/1‑128 (MQ=255)
                                                                                                        tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGT‑TGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                  >  1:794522/1‑127 (MQ=255)
                                                                                                              aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACatat                             >  1:331022/1‑141 (MQ=255)
                                                                                                                gCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAg                           <  1:849353/141‑1 (MQ=255)
                                                                                                                      aTTTAGACGCGGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAc                     >  1:228735/1‑141 (MQ=255)
                                                                                                                       tttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAcc                    <  1:313939/141‑1 (MQ=255)
                                                                                                                                       aggTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCa    >  1:104774/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                              
ATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAT‑TGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACT  >  CP000730/1929721‑1929998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑