Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	396,851	G→A	G28S (GGT→AGT)	ywbN →	iron (Fe2+)‑dependent Dyp family peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	396,851	0	G	A	100.0%	137.2 / NA	47	G28S (GGT→AGT)	ywbN	iron (Fe2+)‑dependent Dyp family peroxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/19);  total (28/19)

GATGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCGGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTACCACACCCACGCAA  >  CP000730/396717‑396985                                                                                                                                       |                                                                                                                                       gATGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTg                                                                                                                                  >  1:618972/1‑141 (MQ=255)   tGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCa                                                                                                                                >  1:314216/1‑141 (MQ=255)       gTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTg                                                                                                                            >  1:196084/1‑141 (MQ=255)        ttAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCGATTgg                                                                                                                           >  1:232363/1‑141 (MQ=255)        ttAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTgg                                                                                                                           >  1:796642/1‑141 (MQ=255)        ttAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTgg                                                                                                                           >  1:385746/1‑141 (MQ=255)        ttAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTAGCAATTgg                                                                                                                           >  1:188684/1‑141 (MQ=255)                  aGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTCAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAgtggt                                                                                                                 <  1:874827/141‑1 (MQ=255)                  aGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAAAGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAgtggt                                                                                                                 <  1:65370/141‑1 (MQ=255)                    gagaGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGCCGGTGCCAGTGTTGCAATTGGCGCAAGTGgcgt                                                                                                               >  1:713807/1‑141 (MQ=255)                         ccACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTa                                                                                                          >  1:375291/1‑141 (MQ=255)                               aGTAAAATGGCGGTGATTACCGAACGACAAATTATAAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATCGGCGCTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCAtgt                                                                                                    <  1:30576/141‑1 (MQ=255)                                gTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCAtgtg                                                                                                   <  1:125228/141‑1 (MQ=255)                                gTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCAtgtg                                                                                                   <  1:634282/141‑1 (MQ=255)                                    aaTGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCt                                                                                               <  1:135684/141‑1 (MQ=255)                                        gCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTGGCATGTGGTCTTTCa                                                                                           >  1:846587/1‑141 (MQ=255)                                        gCTGTGATTACTGAATGACAAATTAGGAATAAGTTAGCGATAGTACGCAATCTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCa                                                                                           <  1:671299/141‑1 (MQ=255)                                         cTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCCATTGGGGCAAGTGGTGTTGGTAGCATGTGGTCTTTCaa                                                                                          >  1:210907/1‑141 (MQ=255)                                         cTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCaa                                                                                          <  1:408404/141‑1 (MQ=255)                                          tgtgATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTAGATTTTTGAAAATGTTAGGTATTGGAGGTGCCAGTGTTGCGATTGGCGCAACTGGTGTTAGTAGCATGTGGTCTTTCaaa                                                                                         <  1:599670/141‑1 (MQ=255)                                          tgtgAATACCGAAGCACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCaaa                                                                                         <  1:411079/141‑1 (MQ=255)                                            tgATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATc                                                                                       <  1:813511/141‑1 (MQ=255)                                            tgATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATc                                                                                       <  1:163405/141‑1 (MQ=255)                                               ttACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTATTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAACTCAAt                                                                                    >  1:734963/1‑141 (MQ=255)                                                 aCTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGt                                                                                  >  1:361237/1‑141 (MQ=255)                                                 aCTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGt                                                                                  <  1:800171/141‑1 (MQ=255)                                                 aCTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATAAATGt                                                                                  >  1:649318/1‑141 (MQ=255)                                                  cTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGtt                                                                                 >  1:707716/1‑141 (MQ=255)                                                           aaaTTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACAcc                                                                        >  1:356852/1‑141 (MQ=255)                                                                    aCAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATcc                                                               >  1:804620/1‑141 (MQ=255)                                                                           aaCGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCACTGTTCAATACACCAGAAGATCCGGaaaaa                                                        >  1:15378/1‑141 (MQ=255)                                                                            aCGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACCCCAGAAGATCCGGAAAAAg                                                       >  1:113569/1‑141 (MQ=255)                                                                            aCGATAGTACGCAATTTTCAAGACGGACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAg                                                       <  1:271341/141‑1 (MQ=255)                                                                                 aGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACAACAGAAGATCAGGAAAAAGATGCg                                                  >  1:616519/1‑141 (MQ=255)                                                                                 aGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGATATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCACATCAATGTTCAATACACGAGAAGGGCCGGAAAAAGATGCg                                                  >  1:229141/1‑141 (MQ=255)                                                                                     cGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGTATATCCGGAAAAAGATGCGTATg                                              <  1:542703/141‑1 (MQ=255)                                                                                           tttCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAACACGATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGACtttt                                        >  1:123695/1‑141 (MQ=255)                                                                                           tttCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACAGGAAGGTCAGGAAAAAGATGCGTATGAAtttt                                        >  1:886746/1‑141 (MQ=255)                                                                                               aaGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATgg                                    >  1:135739/1‑141 (MQ=255)                                                                                                          ttttGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAAc                         <  1:702672/141‑1 (MQ=255)                                                                                                          tgttGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAAc                         <  1:299432/139‑1 (MQ=255)                                                                                                             tGAAAATGTTAGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAg                      <  1:74059/141‑1 (MQ=255)                                                                                                                       aGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTACcac            >  1:431639/1‑141 (MQ=255)                                                                                                                       aGGTATTGGCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTACcac            >  1:616923/1‑141 (MQ=255)                                                                                                                             tGGCGGGGCCAGTGTTGAAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTATTTTAATTCAATGGTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTAAGGTAAAGTGCAACCAGGCATTACCACACCCAc      <  1:212977/141‑1 (MQ=255)                                                                                                                               gCGGTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTAccacgcccacgc    >  1:182555/1‑141 (MQ=255)                                                                                                                                 ggTGCCAGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAAGGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGGAACCAGGCATTACCACACCCACGCaa  >  1:559890/1‑141 (MQ=255)                                                                                                                                       |                                                                                                                                       GATGCTGTTAATGCATTAGGAGAGCCACTAAGTAAAATGGCTGTGATTACTGAATGACAAATTATGAACAAGTTAACGATAGTACGCAATTTTCAAGACGTACATTTTTGAAAATGTTAGGTATTGGCGGTGCCGGTGTTGCAATTGGCGCAAGTGGTGTTGGTAGCATGTGGTCTTTCAAATCAATGTTCAATACACCAGAAGATCCGGAAAAAGATGCGTATGAATTTTATGGTAAAGTGCAACCAGGCATTACCACACCCACGCAA  >  CP000730/396717‑396985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑