Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	665,392	T→G	L79* (TTA→TGA)	iolS →	possible acetytransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	665,392	0	T	G	100.0%	88.6 / NA	27	L79* (TTA→TGA)	iolS	possible acetytransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/15);  total (12/15)

CGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAA  >  CP000730/665254‑665521                                                                                                                                           |                                                                                                                                  cGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgat                                                                                                                                 >  1:356275/1‑141 (MQ=255)  gACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGGAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATgata                                                                                                                                >  1:598950/1‑141 (MQ=255)   acaacaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGatat                                                                                                                               >  1:929876/1‑141 (MQ=255)      acaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATAtttt                                                                                                                            >  1:769286/1‑140 (MQ=255)      acaGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTa                                                                                                                            <  1:153332/141‑1 (MQ=255)               cACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCAt                                                                                                                   <  1:845212/141‑1 (MQ=255)                         aaGCTTCAAATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAc                                                                                                         <  1:512336/141‑1 (MQ=255)                               cATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAAACAGAAt                                                                                                   >  1:116834/1‑141 (MQ=255)                                      tGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACa                                                                                            >  1:900064/1‑141 (MQ=255)                                                    agaaaagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTa                                                                              >  1:492305/1‑141 (MQ=255)                                                        aagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGc                                                                          >  1:241242/1‑141 (MQ=255)                                                        aagaCTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTAAAGc                                                                          >  1:241452/1‑141 (MQ=255)                                                                 aGAACAATTATTTTTAGTCGTTGAAGAAGTTAACGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTACTAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTaaa                                                                 <  1:230288/141‑1 (MQ=255)                                                                 aGAACAATTAGTCTTACTGGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTaaa                                                                 <  1:928996/141‑1 (MQ=255)                                                                                     ttGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTg                                             <  1:223681/141‑1 (MQ=255)                                                                                     ttGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTg                                             <  1:572543/141‑1 (MQ=255)                                                                                              gTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAt                                    <  1:700823/141‑1 (MQ=255)                                                                                                aaTGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttt                                  <  1:63959/141‑1 (MQ=255)                                                                                                aaTGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttt                                  <  1:925453/141‑1 (MQ=255)                                                                                                aaTGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttt                                  <  1:337018/141‑1 (MQ=255)                                                                                                aaTGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAAttt                                  <  1:314765/141‑1 (MQ=255)                                                                                                 aTGACATCGTTGGCTTTGCTAACTTTATTTACGATGAAGAATGATGTTTATCATCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTg                                 >  1:880595/1‑141 (MQ=255)                                                                                                   taaaacGTTGGCTTTGCTATCGTTATGTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCAGTTTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGaa                               <  1:88314/136‑1 (MQ=255)                                                                                                   gACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTGGAAGATAAATTTGaa                               <  1:689350/141‑1 (MQ=255)                                                                                                               tttGCTAACTTTATTTACGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCACCATACAGGTTATGGTACAGCATTGTTAAAGGACGGATTATAACGTTTGGAAGATAAATTTGAAGGTGTGTCCTTa                   >  1:437537/1‑141 (MQ=255)                                                                                                                    tAACTTTATTTACGGTGAAGAAGGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGt              <  1:878362/141‑1 (MQ=255)                                                                                                                           atttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGatact       >  1:360226/1‑139 (MQ=255)                                                                                                                           atttaCGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATCATCACGTTTTGAGGATAAAGTGGAGGGTGTTTACTGTGAAGTAGttaat       >  1:228938/1‑141 (MQ=255)                                                                                                                                cGGTGAAGAATGATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTCGAAGTAGAAAATaaaaa  >  1:197492/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                  CGACAACAGTTAATCACTTGTTAGAAGCTTCATATAATGAACATCATTTAAAGAAAAGACTTCAAGAACAATTATTCTTAGTCGTTGAAGAAGGTAATGACATCGTTGGCTTTGCTAACTTTATTTACGGTGAAGAATTATATTTATCAGCTCATTATGTTAAACCAGAATCGCAACATACAGGTTATGGTACAGCATTGTTAAATGAAGGATTATCACGTTTTGAAGATAAATTTGAAGGTGTTTACTTAGAAGTAGATAATAAAAA  >  CP000730/665254‑665521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑