Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,440,007 Δ1 bp 100% coding (34/201 nt) cspA ← cold shock protein CspA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,440,0070C.100.0% 63.8 / NA 15coding (34/201 nt)cspAcold shock protein CspA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (8/7);  total (8/7)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAAT  >  CP000730/1439876‑1440140
                                                                                                                                   |                                                                                                                                     
gTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATATGTCATTTTGTCCTTCACCTTCGCCATAGCTGTATCCTTTTTAGCGTTAAAc                                                                                                                             >  1:130124/1‑141 (MQ=255)
gTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGGCATTTTCTGCTTCCACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAAc                                                                                                                             >  1:130138/1‑141 (MQ=255)
                     cTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAACTGTACGAATACGTCATTTTCTCCTTCACCTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACAATTTAACTGTACCTTTTTTc                                                                                                        >  1:113303/1‑141 (MQ=255)
                                       ttttAATGATTTGTAACCATCTTGGTTCATCGTTGAAAAATGTACGAATTCGTAATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTAGCTTGTTTCATAATCTGAAACCTCCaa                                                                                      <  2:54912/138‑1 (MQ=255)
                                            aTGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTa                                                                                 <  2:233590/141‑1 (MQ=255)
                                                   gTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAattca                                                                          >  1:74383/1‑141 (MQ=255)
                                                             tGGTTAATTGCTGCAAAATGTACGAATACGTCATTTTCTCCTTCAACGTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGc                                                                <  2:181587/141‑1 (MQ=255)
                                                                ttAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTa                                                             <  2:273306/141‑1 (MQ=255)
                                                                      gCTGAAAAATGTACGAACACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAGTTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCa                                                       <  1:131628/141‑1 (MQ=255)
                                                                                     aaTACGTCATTTTCTCCTTAAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATaa                                        >  1:254687/1‑140 (MQ=255)
                                                                                         cGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGc                                    <  2:48170/141‑1 (MQ=255)
                                                                                            cATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCaaa                                 >  1:44276/1‑141 (MQ=255)
                                                                                                    ccTTCAACTTCGATACAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTc                         <  1:63860/141‑1 (MQ=255)
                                                                                                                aTAAAGCCGAATCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATATGAAACCACCAAGACGAAAATTCATTCAATATGCTTATTCGCAGATTACAAAAAATCCAGGCAAATTTCACTCAAAAATTCTTTg             >  2:76169/1‑141 (MQ=255)
                                                                                                                           tCCTTTTTAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCGAATATTCTTTGCAATATGGaat  >  1:44441/1‑141 (MQ=255)
                                                                                                                                   |                                                                                                                                     
GTCGCCTTCAACTACTTCAAACTCAACAGCTTGACCTTCTTCTAATGATTTGTAACCATCTTGGTTAATTGCTGAAAAATGTACGAATACGTCATTTTCTCCTTCAACTTCGATAAAGCCGAATCCTTTTTCAGCGTTAAACCATTTAACTGTACCTTGTTTCATAATCTGAAACCTCCAAGACTAAAATTCATTCAATATGCTTATTCGCATATTACAAAAAATACATGCAAATTTCACTCAAATATTCTTTGCAATATGGAAT  >  CP000730/1439876‑1440140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: