| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,233,332 | C→A | 57.9% | P413T (CCT→ACT) | prkC → | non‑specific serine/threonine protein kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,233,332 | 0 | C | A | 57.9% | 2.1 / 29.2 | 26 | P413T (CCT→ACT) | prkC | non‑specific serine/threonine protein kinase |
| Reads supporting (aligned to +/- strand): ref base C (3/8); new base A (8/7); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01 | |||||||||||
ATGGCAATGTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGC > CP000730/1233200‑1233468 | atggcaatgTTTGGTAATAAATACGAAGAGACACCTGATGTAAGCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGGAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAAt < 2:311354/141‑1 (MQ=255) gcaatgTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGaa > 1:58574/1‑141 (MQ=255) tGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGTTAAATATACTGAAAATGAAATTATTaa < 2:73509/141‑1 (MQ=255) gTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGa < 1:278112/141‑1 (MQ=255) taataaATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGAc > 1:11711/1‑141 (MQ=255) aaataCGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACt > 1:145573/1‑141 (MQ=255) aaGACAAACCTGATGTAATCGGGAAATCTGTAAAAGAAGAAGAGCAAATATTCAATAAAAACAACCTGAAATCGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATa < 2:281964/141‑1 (MQ=255) gaCACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATAATgg < 1:248515/141‑1 (MQ=255) cacCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTg > 1:152346/1‑141 (MQ=255) aaTCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTCAGACAACTCCTAATACTGGTGAACGTGTTGa > 2:54878/1‑141 (MQ=255) tCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAAc > 2:143802/1‑141 (MQ=255) gAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACgtgg < 1:168704/141‑1 (MQ=255) aTCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGa < 2:11711/141‑1 (MQ=255) tCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGATGAc > 2:65431/1‑141 (MQ=255) tAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAgtg > 1:336709/1‑141 (MQ=255) aaaGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGtt < 1:185404/141‑1 (MQ=255) aaagCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGAt < 2:99328/140‑1 (MQ=255) agagCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAAGATACTGAAAATGAAATTATTAAGACAACTCATAATACTGGTGAACGTGTTGAACGTGTTAGAACTGTTGAtgttgt > 2:201970/1‑141 (MQ=255) acaacaatacAATAAAAACAACCTGAGATTGGGTAAAATTTCTAGAACTAAAAGTGATAAATATCCGGAAAATGAAATTATTAATACAACTCCTAATACTGGTGAACCTGTTGAACGTGGTGACAGTGTTGATGTTGTtat < 2:24439/132‑1 (MQ=255) ttCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAg < 1:247003/141‑1 (MQ=255) aTAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGcc < 1:318251/141‑1 (MQ=255) aTTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAAt < 2:189828/141‑1 (MQ=255) gTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCaa < 2:58574/141‑1 (MQ=255) aaTTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGt < 2:322802/141‑1 (MQ=255) ttATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTAcc > 2:129319/1‑141 (MQ=255) atatACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGc > 1:260205/1‑141 (MQ=255) | ATGGCAATGTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAAATGCCAAATGTCATTGGTTTACCTAAGGAGGAAGC > CP000730/1233200‑1233468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |