Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	71,870	T→C	8.9%	A368A (GCA→GCG)	arcD1 ←	arginine/ornithine APC family amino acid‑polyamine‑organocation transporter, antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	71,870	0	T	C	8.9%	125.2 / 4.1	45	A368A (GCA→GCG)	arcD1	arginine/ornithine APC family amino acid‑polyamine‑organocation transporter, antiporter
Reads supporting (aligned to +/- strand):  ref base T (21/20);  new base C (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

ATCCAGCTGCATAAACCAACCAAATTGTGTAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAAC  >  CP000730/71731‑72005                                                                                                                                            |                                                                                                                                        atcCAGCTGCATAAACAAACCAAATTGTGTAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGGATTAAGATTg                                                                                                                                        >  1:204705/1‑141 (MQ=255)     aGCTGCATAAACCAACCAAATTGTGTAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACt                                                                                                                                    <  1:18955/141‑1 (MQ=255)      gCTGCATAAACCAACCAAATTGTGTAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACtt                                                                                                                                   >  2:91906/1‑141 (MQ=255)          cATAAACCAACCAAATTGTGTAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATg                                                                                                                               >  1:213014/1‑141 (MQ=255)                   accaaATTGGGTAAATAGATTCAGTATTTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGaaa                                                                                                                      <  2:54792/141‑1 (MQ=255)                      aaaTTGTGTAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACg                                                                                                                   >  1:178995/1‑141 (MQ=255)                       aaTTGTGTAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCCAGTAAAAGCga                                                                                                                  >  2:329544/1‑140 (MQ=255)                           gtgtACACAGATAGAAGAATACCAACTAGCCATTGCTGTAAATTAGCTTTAGATTTACTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAAc                                                                                                              <  1:155113/141‑1 (MQ=255)                           gtgtAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAAc                                                                                                              <  1:301875/141‑1 (MQ=255)                            tgtAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACt                                                                                                             <  2:227472/141‑1 (MQ=255)                            ggtAAATAGATGCAATAATTCCGATTATCCATTGTTCGAAATGTGATTTAAAGTTATTTTGAATAGTATATTAAAAAAAGAAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACt                                                                                                             <  1:338857/140‑1 (MQ=255)                             gtAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTg                                                                                                            <  1:329360/141‑1 (MQ=255)                              tAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATCGCACTTGATGCAAGTGAAAACGCAAACTGa                                                                                                           >  2:316931/1‑141 (MQ=255)                              tAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATCGCACTTGATGCAAGTGAAAACGCAAACTGa                                                                                                           >  2:316915/1‑141 (MQ=255)                               aaaTAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGAt                                                                                                          <  2:178995/141‑1 (MQ=255)                                 atggATGCAATAATTCCTTTTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACGGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAg                                                                                                        <  2:117868/138‑1 (MQ=255)                                      tGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACt                                                                                                   <  1:132286/141‑1 (MQ=255)                                        cAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGAGTTATTTTGAATAGTATATTTAACCCGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAATCTGATATGCACTAt                                                                                                 >  1:246601/1‑141 (MQ=255)                                        cAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGAATTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTAt                                                                                                 >  1:246622/1‑141 (MQ=255)                                           taatTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTGGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGCGAAAACGCAAACTGATAGGCACTATCTg                                                                                              >  1:194498/1‑141 (MQ=255)                                                 cTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAaaca                                                                                        >  1:197030/1‑141 (MQ=255)                                                          aTTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTaa                                                                               >  2:108004/1‑141 (MQ=255)                                                           ttGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAAt                                                                              >  1:177068/1‑141 (MQ=255)                                                               tttAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACCTACTAATTaaa                                                                          >  1:9137/1‑141 (MQ=255)                                                                ttAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTATAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTaaaa                                                                         <  1:111737/141‑1 (MQ=255)                                                                 tAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTaaaaa                                                                        <  2:234663/141‑1 (MQ=255)                                                                            tAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAAAATACTAATTAAAAATAACTGAACTa                                                             >  2:123594/1‑141 (MQ=255)                                                                                   atttgaaaTGGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGAACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATAtt                                                      <  2:149176/135‑1 (MQ=255)                                                                                      ttGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGt                                                   >  1:178844/1‑141 (MQ=255)                                                                                                tatTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTaaa                                         >  2:314529/1‑141 (MQ=255)                                                                                                   ttAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCg                                      >  1:215078/1‑141 (MQ=255)                                                                                                   ttAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCg                                      >  1:214134/1‑141 (MQ=255)                                                                                                    tagccTGGTAAAAAGCACTGAGAGTAGATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTACTATCTTAGTCATGTTTaaaccat                                     <  2:246601/138‑5 (MQ=255)                                                                                                    tAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTATTATATTAAtaccaaggaatgact                                     <  2:246622/141‑14 (MQ=255)                                                                                                          ggTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCg                               <  2:15925/141‑1 (MQ=255)                                                                                                          ggTAAAAAGCACTGAGTGTATATGGAATTAAGATCGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCg                               <  1:316931/141‑1 (MQ=255)                                                                                                          ggTAAAAAGCACTGAGTGTATATGGAATTAAGATCGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCg                               <  1:316915/141‑1 (MQ=255)                                                                                                              aaaaGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGc                           <  1:28641/141‑1 (MQ=255)                                                                                                                       gAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCtt                  <  2:161853/141‑1 (MQ=255)                                                                                                                         gtgtATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAGTAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTAt                <  1:263360/141‑1 (MQ=255)                                                                                                                           gtATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTTACCTGAGCTTTATTCTTAttt              >  2:148026/1‑141 (MQ=255)                                                                                                                             atatGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTc            <  1:91906/141‑1 (MQ=255)                                                                                                                                tGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCttt         >  1:105862/1‑141 (MQ=255)                                                                                                                                   aaTTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGc      >  1:83502/1‑141 (MQ=255)                                                                                                                                       aaGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAAc  >  1:97711/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                        ATCCAGCTGCATAAACCAACCAAATTGTGTAAATAGATGCAATAATTCCTATTATCCATTGTTTTAAATTAGCTTTAGATTTATTTTGAATAGTATATTTAACCTGGTAAAAAGCACTGAGTGTATATGGAATTAAGATTGCACTTGATGCAAGTGAAAACGCAAACTGATAGGCACTATCTGTAAACAACATACTAATTAAAAATAACTGAACTAATATATTAGTAATAATTAAAGCGTTGACCGGAGCTTTATTCTTATTTTCTTTAGCAAAC  >  CP000730/71731‑72005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑