| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,279,705 | G→A | 100% | intergenic (+1373/+6659) | mtlD → / ← glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,279,705 | 0 | G | A | 98.6% | 96.4 / ‑6.4 | 38 | intergenic (+1373/+6659) | mtlD/glmM | mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (17/18); minor base T (0/2); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT > CP000730/2279571‑2279841 | tgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttg > 1:24422/1‑141 (MQ=255) ttattaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTCCTGTTGTTTcc > 1:342463/1‑140 (MQ=255) ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 1:277847/141‑1 (MQ=255) tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGGTGTTACTGTTGTTTCACCTGTTGCCGCATc > 1:291637/1‑141 (MQ=255) cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc > 1:148210/1‑141 (MQ=255) cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc > 1:264069/1‑141 (MQ=255) cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc < 1:274332/141‑1 (MQ=255) cTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACttt > 2:159619/1‑139 (MQ=255) tGGTTTACTTGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtatt < 1:160722/141‑1 (MQ=255) ggTTTACTAGTTACATCGTTATCCATTGCCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtcttt > 2:73563/1‑140 (MQ=255) aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTg‑g > 2:124189/1‑141 (MQ=255) tAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑gtg > 1:293261/1‑141 (MQ=255) cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGTTTTATCATTTGCCTCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCTTCCCTTTTCTTTT‑GTTTTGTCGcag > 2:72695/1‑141 (MQ=255) cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGgag > 1:317602/1‑141 (MQ=255) gTTATCCCTTGTCCGACGGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGgaga < 1:157242/141‑1 (MQ=255) tATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAg < 1:184847/141‑1 (MQ=255) tATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAg > 1:297875/1‑141 (MQ=255) gccATTGTTGGCCGGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCg < 2:199716/140‑1 (MQ=255) cATTGTCGGACTGTTTGTTGATGCATATACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTc > 2:213456/1‑141 (MQ=255) aaTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGCAGCGTc > 2:284472/2‑141 (MQ=255) aTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTcg > 1:61109/1‑140 (MQ=255) ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAg < 1:288270/141‑1 (MQ=255) tgttgATGCATGTACGCTAGTCTTGTTATTAGCTTGCGGTTTCTCATTTGGATCATGAGTTGCTGATGTTTCTGTTGTTTCACATGTTGCCGCATCACTATTATTTG‑GTGTGGTCGGAGAAGCGTCTGCTTTGCCATTAGc < 2:78342/141‑1 (MQ=255) tgctgtaTCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgt < 2:19251/135‑1 (MQ=255) tccacacAAGAATTGTTATTAGCTTGCGGTTTATTATTTGCTGCATCAGTAGATACTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCa < 2:207040/138‑1 (MQ=255) cTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTATCGTCTCAg > 2:135314/1‑141 (MQ=255) cacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATa < 2:2645/141‑1 (MQ=255) ttgAATTGTCGTTAGCTTGCGGTTTTTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt < 2:298187/139‑1 (MQ=255) tAGAATGGTTATTTGCTTGCGGTTTTTCTTTTGCCTCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGTTACGt < 2:41008/141‑1 (MQ=255) tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGCCGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc > 1:63372/1‑141 (MQ=255) ttGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtt < 1:25529/141‑1 (MQ=255) tGCGGTGTATTATTTGCATTATTAGTTGCTGATGTTACTGTTGTTGCCCCTGTTGCCGCTTCACTATTATTTG‑GTGGTGTCGGAGAAGCGTCTGCTTTGCCATTGGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAttt < 2:111132/141‑1 (MQ=255) ggTTTATTATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTGACCTGTTGCCCCATCACTATTATTTG‑GTGTTGTCTGAGAAGCGTCGGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt < 2:144357/141‑1 (MQ=255) ttGCTGATGTTTCTTGCGTTTCACCTGTTGCCGCATATCTATTCTATGCGTG‑GGGCGGAGGGTCGTCTGCTTTGCCATTCGCTGTCGTCCCAGATACGTTCGGTTGTCCAGTACTTTCTGGTGTTGCATTAGCATTTGAAt < 2:169839/141‑1 (MQ=255) ttaTGTTACTGTTGTTTCTCCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc < 2:21905/139‑1 (MQ=255) ggtGTTACTTTTGTTTCACCTCTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCt < 1:23414/139‑1 (MQ=255) aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTg > 1:92051/1‑141 (MQ=255) tGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGt < 2:297875/141‑1 (MQ=255) | TGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTG‑GTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT > CP000730/2279571‑2279841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |