| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 496,458 | C→A | 17.0% | S220I (AGC→ATC) | gltC ← | glutamate synthase operon transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 496,458 | 0 | C | A | 17.0% | 74.3 / 6.7 | 28 | S220I (AGC→ATC) | gltC | glutamate synthase operon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (13/10); new base A (2/3); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01 | |||||||||||
AGCACATATCGTTCTTTTTAATTCAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAACTGAATTAATGGTGGGTGTGTCGCCATCGTAATTGGATCGTC > CP000730/496322‑496597 | aGCACATATCGTTCTTTTTAATTCAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACt > 2:108927/1‑141 (MQ=255)aGCACATATCGTTCTTTTTAATTCAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACt > 2:130915/1‑141 (MQ=255) cGTTCTTTTTAATTCAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATAGTGta > 2:240054/1‑141 (MQ=255) tttAATTCAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCt > 1:105668/1‑141 (MQ=255) tCAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACGTATTGTGTATACATCTGATTTa < 2:377540/141‑1 (MQ=255) cAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAg > 1:101530/1‑141 (MQ=255) cAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGATACTGATTGTGTATACATCTGATTTAg < 2:157511/141‑1 (MQ=255) aTGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTCGTTGGCTACTGATTGTGTATACATCTGATTTAGTAAt < 1:272498/141‑1 (MQ=255) aaTTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAAACTTCTTTTTGCCAAAGGAGATACTGCGCAAGTGGTTGGCTACTGCTTGTGTATACATCTGATTTAGTAATCTTgcgc > 1:93542/1‑141 (MQ=255) ttttATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCaa > 2:197490/1‑141 (MQ=255) tataTCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTg < 1:59843/141‑1 (MQ=255) atatCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGATACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGt < 2:162806/141‑1 (MQ=255) tctatcCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGtttt > 1:32310/1‑141 (MQ=255) ctatcCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGttttt > 2:212817/1‑141 (MQ=255) tatcCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGtttttt > 2:157434/1‑141 (MQ=255) tcCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTa < 1:346592/141‑1 (MQ=255) tttAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTc > 1:19050/1‑141 (MQ=255) cTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATCTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATg > 2:360784/1‑141 (MQ=255) tgatgTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCt < 1:77310/141‑1 (MQ=255) gatgTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGATACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTc < 2:24354/141‑1 (MQ=255) atgTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCa < 1:303651/141‑1 (MQ=255) tAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAAc > 1:89351/1‑141 (MQ=255) ttttGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAACTGAATTAAt < 1:333537/141‑1 (MQ=255) gagaTACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAACTGAATTAATGGTGGgtgtgt < 1:108927/141‑1 (MQ=255) cTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAACTGAATTAATGGTGGGTGTGTCGCCAt < 2:37589/141‑1 (MQ=255) ttGGATACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAACTGAATTCATGGTGGGTGTGTCGCCATCGTAATTGGATc > 2:128021/1‑141 (MQ=255) ttGGATACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAACTGAATTAATGGTGGGTGTGTCGCCATCGTAATTGGATc > 2:151792/1‑141 (MQ=255) gCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAACTGAATTAATGGTGGGTGTGTCGCCATCGTAATTGGATCGTc < 1:360784/141‑1 (MQ=255) | AGCACATATCGTTCTTTTTAATTCAGTATGATCTAATTTTATATCTATCCATGATTTAGATTCTGGTAAATGTATATTTTGTGATGAAATGATGTAACCTTCTTTTTGACGAAGGAGATACTGCGCAAGTGGTTGGCTACTGATTGTGTATACATCTGATTTAGTAATCTTGCGCAATTGTTTTTTTACAGTTTCGGCAAATGGTGCCAAGCAATAAATATGACTATGCTCAAACTGAATTAATGGTGGGTGTGTCGCCATCGTAATTGGATCGTC > CP000730/496322‑496597 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |