| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,013,179 | A→T | 28.0% | Q19H (CAA→CAT) | yfhP → | possible metal‑dependent hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,013,179 | 0 | A | T | 28.0% | 41.3 / 19.2 | 25 | Q19H (CAA→CAT) | yfhP | possible metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (9/9); new base T (4/3); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTTAGTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATAGAGGTATCACGCA > CP000730/2013041‑2013313 | tGGTTAGTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGa < 2:21643/141‑1 (MQ=255)tGGTTAGTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGa < 1:49697/141‑1 (MQ=255) gTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGc < 1:336493/141‑1 (MQ=255) aaGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGc > 2:86044/1‑141 (MQ=255) aaTTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTa < 2:87663/141‑1 (MQ=255) tttAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACg > 1:91789/1‑141 (MQ=255) tttAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACg > 1:91829/1‑141 (MQ=255) ttAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGt > 2:33490/1‑141 (MQ=255) aaaTTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGc > 1:94929/1‑141 (MQ=255) tataATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCt < 1:351558/141‑1 (MQ=255) atatTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGGCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACCGCTACAACCCTTATCGTTGGTTCAttt > 2:131314/1‑140 (MQ=255) ttatGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAAtt > 1:133551/1‑141 (MQ=255) aaaTGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGa > 1:39958/1‑141 (MQ=255) aGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCGTACAGCACTTGCAACTCATGATCCTTCAAGTGCTTCTACTTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATgggg > 1:225490/1‑141 (MQ=255) gAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATa > 2:261717/1‑141 (MQ=255) aaCTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATAc > 2:373245/1‑141 (MQ=255) cTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCttaaattaa < 2:273917/141‑1 (MQ=255) gCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACa < 2:95128/141‑1 (MQ=255) ggggTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATAtt < 1:308508/141‑1 (MQ=255) ggTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTc < 2:119775/141‑1 (MQ=255) gTGGGCCTTACAGCACTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCg < 2:327759/141‑1 (MQ=255) tGGGCCTTACCGCACTTGCAACTCCTGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTCATTCCTGATGGGGATACTCTGCTTAAATTAAAGGACAATGCCACATATATTTCGc < 2:225490/141‑1 (MQ=255) cTTGCAACTCATGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATAGAGGTATc < 2:133551/141‑1 (MQ=255) aaCTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATAGAGGTATCACGCa > 2:371526/1‑141 (MQ=255) aaCTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATAGAGGTATCACGCa > 2:57985/1‑141 (MQ=255) | TGGTTAGTATGTAAGTTAATTTAGTTATTTGCGAAATTGGATTATAATAGTATATATAATATTATGAAATGAGTGAACTGATATGGACACTGCAACACATATCGCAATTGGGGTGGGCCTTACAGCACTTGCAACTCAAGATCCAGCAATGGCTTCTACGTTTGGTGCAACAGCTACAACCCTTATCGTTGGTTCATTAATTCCTGATGGGGATACTGTTCTTAAATTAAAGGACAATGCAACATATATTTCGCATCATAGAGGTATCACGCA > CP000730/2013041‑2013313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |