| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,028,542 | G→C | 22.2% | L35V (CTC→GTC) | yvqF ← | hypothetical membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,028,542 | 0 | G | C | 22.2% | 80.3 / 22.2 | 36 | L35V (CTC→GTC) | yvqF | hypothetical membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (18/10); new base C (6/2); total (24/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01 | |||||||||||
AGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATAT > CP000730/2028404‑2028678 | aGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTa > 2:123980/1‑141 (MQ=255) gatgatgatTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTaa > 2:278099/1‑141 (MQ=255) tgatTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGc > 1:285795/1‑141 (MQ=255) ttGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTAGGTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCaa > 2:175080/1‑141 (MQ=255) cTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTc < 1:101218/141‑1 (MQ=255) aatGTGAATGCAATTAATAGCGCGCCTATACAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATgtag < 2:251320/141‑1 (MQ=255) tgtgAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGACCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATgtagta < 2:123365/141‑1 (MQ=255) tgtaAATACAATTAATAGCGCGACTATCCAAAACGCCAAAAGGACACATATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCAAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATgtagta < 2:240670/141‑1 (MQ=255) gtgAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTaa > 1:28914/1‑141 (MQ=255) aaTGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTaaaaa > 2:4455/1‑141 (MQ=255) gCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTg < 2:134838/141‑1 (MQ=255) aTTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCa > 2:358115/1‑141 (MQ=255) cgcgcCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCAt > 2:12286/1‑141 (MQ=255) ccTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAAt > 2:184723/1‑141 (MQ=255) tATCCAAAACGCCAAAAGGCAACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTAAAAAGCAAAAAAATTAAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGc < 2:208975/141‑1 (MQ=255) aTCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCa > 2:63097/1‑141 (MQ=255) gacAAAAAGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTAAAAAGACAATATTTTAAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATg < 2:204392/139‑1 (MQ=255) ccAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGa > 1:302845/1‑141 (MQ=255) aaGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGACTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCaa > 2:153405/1‑141 (MQ=255) tATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGata < 1:4455/141‑1 (MQ=255) tatCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTc < 2:39036/141‑1 (MQ=255) atCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCa < 1:301097/141‑1 (MQ=255) ccTACATAAACTCATACACATCCCCATAATAGAACGAGTAAAAAGCCAATTTTCTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTCATATATATTTGCGTGTCATg > 2:303114/1‑141 (MQ=255) tACATAAACTAATACACATCCCAATAATAGACCGACTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGtt > 2:44178/1‑141 (MQ=255) aCATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGttt > 2:20105/1‑141 (MQ=255) cTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCgccgcc > 1:227202/1‑141 (MQ=255) aaTACACATCCCAATAATAGAACGACTAAAAAGCCAATTTTTTTTAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATCTATATTTCTGACTCATGTTACGCAGCCtt > 2:130269/1‑141 (MQ=255) aaTACACATCCCAATAATAGAACGACTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCtt > 1:233613/1‑141 (MQ=255) ccAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcac > 1:63221/1‑141 (MQ=255) cAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATCTATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcaca < 2:319753/141‑1 (MQ=255) cAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcaca > 2:370499/1‑141 (MQ=255) cAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcaca > 1:178838/1‑141 (MQ=255) cAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCcaca > 1:178872/1‑141 (MQ=255) aataatAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg < 1:92699/141‑1 (MQ=255) aataatAGAACGACTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAg > 1:89155/1‑141 (MQ=255) aCGAGTAAAAAGCCAATTTTTTTTTTTTTTTTTTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCTTCTGCGTTCatat > 2:92876/1‑141 (MQ=255) | AGATGATGATTGTATACTTATTAGACAATAATGTGAATGCAATTAATAGCGCGCCTATCCAAAACGCCAAAAGGCCACGTATTTTATGAAAATAAAGATATCCTACATAAACTAATACACATCCCAATAATAGAACGAGTAAAAAGCCAATTTTTTCAAAAAATATGTAGTAAAAATTGGCAATAATCATTAATGCAGTAAAAATGATCAACATTTGCGTTGATATATATTTGTGTGTCATGTTTCGCCGCCTTTCTATGCCACAGCGTTCATAT > CP000730/2028404‑2028678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |