Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	39,246	C→T	A630T (GCT→ACT)	mecA ←	penicillin binding protein 2 prime

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	39,246	0	C	T	100.0%	67.6 / NA	22	A630T (GCT→ACT)	mecA	penicillin binding protein 2 prime
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/9);  total (13/9)

CTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCT  >  CP000730/39119‑39382                                                                                                                                |                                                                                                                                         cTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGttt                                                                                                                             >  1:226509/1‑141 (MQ=255)     tttGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGAt                                                                                                                         <  2:88091/141‑1 (MQ=255)        gTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTAt                                                                                                                      <  2:90360/141‑1 (MQ=255)                    tatCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCat                                                                                                             >  2:182872/1‑138 (MQ=255)                    tatCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCat                                                                                                             <  1:182872/138‑1 (MQ=255)                                          ctcATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCt                                                                                    >  2:188479/1‑141 (MQ=255)                                              tataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCa                                                                                >  2:155439/1‑141 (MQ=255)                                               ataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAg                                                                               <  2:274698/141‑1 (MQ=255)                                               ataGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAg                                                                               <  2:88870/141‑1 (MQ=255)                                                     catcatACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTtctc                                                                         >  2:243375/1‑141 (MQ=255)                                                       tcatACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATACGTTTATCTTGTCCATATTTAACATCAAACGTCTTCTTTATCGTTGTATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCt                                                                       <  1:258186/141‑1 (MQ=255)                                                               tttACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCAt                                                               >  2:266099/1‑141 (MQ=255)                                                                   ccTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCATTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTGGTCTGCCAGTTTTTCCTTGTTTCAttttt                                                           >  2:209705/1‑140 (MQ=255)                                                                       agaTTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGtt                                                       >  1:129287/1‑141 (MQ=255)                                                                                          agcCATTCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGcc                                    <  1:40465/141‑1 (MQ=255)                                                                                               ttCCTTTATCTTGTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTa                               <  2:160743/141‑1 (MQ=255)                                                                                                           gTACATCTTTAACATTAATAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAg                   >  1:207295/1‑141 (MQ=255)                                                                                                           gTACATCTTTAACATTAATAGTCAGCATCATGGTTGGGTTATCTTTATCATATGATATTAACCACTCAATTTGTATGTTAGTTTCACCGTGTTTCATCTTTAGGTCTGCATGATCGGAATTGCCATTTAAGTTTTCAttaa                   >  2:49247/1‑138 (MQ=255)                                                                                                                           aaTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtcttc   >  1:136093/1‑141 (MQ=255)                                                                                                                            aTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAatat       >  1:305065/1‑136 (MQ=255)                                                                                                                            aTAGTCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGATTCATTTTAAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATAtcttct  >  1:254612/1‑141 (MQ=255)                                                                                                                            aTAGTCATCATCATGATTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAatat       <  2:305065/136‑1 (MQ=255)                                                                                                                                |                                                                                                                                         CTGTTTTGTTATTCATCTATATCGTATTTTTTATTACCGTTCTCATATAGCTCATCATACACTTTACCTGAGATTTTGGCATTGTAGCTAGCCATTCCTTTATCTTGTACATCTTTAACATTAATAGCCATCATCATGTTTGGATTATCTTTATCATATGATATAAACCACCCAATTTGTCTGCCAGTTTCTCCTTGTTTCATTTTGAGTTCTGCAGTACCGGATTTGCCAATTAAGTTTGCATAAGATCTATAAATATCTTCT  >  CP000730/39119‑39382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑