| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP000730 | 1,064,016 | G→A | A158V (GCA→GTA) | brpA ← | LytR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,064,016 | 0 | G | A | 96.5% | 94.3 / ‑3.9 | 29 | A158V (GCA→GTA) | brpA | LytR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (18/10); total (19/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
AGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGG > CP000730/1063883‑1064143 | aGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCat > 2:95296/1‑141 (MQ=255) aTTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTcac < 2:80603/141‑1 (MQ=255) tttttGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACataata > 2:241648/1‑141 (MQ=255) aCCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAg > 1:196389/1‑141 (MQ=255) cccTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTTACTCTCACATAATAATCAACAgg > 2:298257/1‑141 (MQ=255) cTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTa < 2:113963/141‑1 (MQ=255) tttaatCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGt > 1:82931/1‑141 (MQ=255) ttACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCt > 2:105447/1‑141 (MQ=255) tACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCtc > 1:256518/1‑140 (MQ=255) gaCCAGTATCATCTGGGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGTGACATTCATGGTAGCTTTTa > 2:138601/2‑141 (MQ=255) tcTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCAtt < 1:124668/141‑1 (MQ=255) ttCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTgg > 2:178748/1‑141 (MQ=255) ttCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTgg < 1:83018/141‑1 (MQ=255) aTTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGacc < 1:230826/141‑1 (MQ=255) aGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGAccacca < 2:88606/140‑1 (MQ=255) aGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGAccacca > 1:88606/1‑140 (MQ=255) gTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgc > 1:93044/1‑141 (MQ=255) tATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgca < 1:95296/141‑1 (MQ=255) tATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgca > 2:218957/1‑141 (MQ=255) tatactatgatgtGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgcatgtgcat > 1:130354/13‑141 (MQ=255) atcatAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATACTTTCATGTTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATAtgcatgtgcatg > 1:234093/1‑141 (MQ=255) ccaccaAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTa < 2:140089/67‑1 (MQ=255) ccaccaAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTa > 1:140089/1‑67 (MQ=255) ccaccaAGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCg > 2:170196/1‑141 (MQ=255) ccaccaAGCTCATTTACAGCCTAGACAAATAATTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCTATTGCGGCAATTGGACTACCATATGCATGTGCATGGGTAATATTATCg > 2:44294/1‑141 (MQ=255) aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAg < 1:38912/141‑1 (MQ=255) aGCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAg > 2:166811/1‑141 (MQ=255) gCTCATTTACAGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGc > 2:7254/1‑141 (MQ=255) aGCCTCGACAAATACTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAgg < 1:281601/141‑1 (MQ=255) | AGCCTCATCACCATTTAATTTTTGGTACCCTTTTTTAATTTTAATCTTACCAGTATCATCTGTGTTAGGTTCATTTAAGTCGTATGGCACATCATAGTATATGCCACCAAGCTCATTTACAGCCTCGACAAATGCTTTCATATTGACTCTCACATAATAATCAACAGGTACATTCATGGTAGCTTCTACCGAATCCATTGCGGCAATTGGACCACCATATGCATGTGCATGGGTAATCTTATCGTAATAGCCAACTTTAGG > CP000730/1063883‑1064143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |