Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,233,332	C→A	P413T (CCT→ACT)	prkC →	non‑specific serine/threonine protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,233,332	0	C	A	100.0%	90.5 / NA	26	P413T (CCT→ACT)	prkC	non‑specific serine/threonine protein kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/16);  total (10/16)

AATGGCAATGTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAA  >  CP000730/1233199‑1233433                                                                                                                                      |                                                                                                      aatggcaatgTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGaaaa                                                                                                >  2:70706/1‑141 (MQ=255)      caatgTTTGGTAATAAATACGAAGAAACACCTGATGTAATCGGGAAATCTGTAAAATAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGaaa                                                                                           <  2:287659/141‑1 (MQ=255)       aatgTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAat                                                                                          <  2:63555/141‑1 (MQ=255)         tgTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAatta                                                                                        >  2:304259/1‑141 (MQ=255)                taataaATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGAc                                                                                 >  2:113802/1‑141 (MQ=255)                taataaATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGAc                                                                                 <  1:206949/141‑1 (MQ=255)                              agaCACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTg                                                                   <  1:106589/141‑1 (MQ=255)                               gaCACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGGAATTGGATAAAATTTCTAGAAGTTATAGTGATAAATGTACTGAAAATGAAATTATTAAGGCAACTCCTAATACTgg                                                                  <  2:197796/141‑1 (MQ=255)                               gaCACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTgg                                                                  >  1:144935/1‑141 (MQ=255)                                     tGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACg                                                            >  2:96831/1‑141 (MQ=255)                                       aTGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACgtg                                                          <  2:46076/141‑1 (MQ=255)                                               gggAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACgt                                                  <  2:180725/141‑1 (MQ=255)                                                  aaaTCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGGAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACgtggt                                               <  2:202384/141‑1 (MQ=255)                                                   aaTCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACgtggtg                                              <  1:157977/141‑1 (MQ=255)                                                     tCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGAc                                            <  2:49670/141‑1 (MQ=255)                                                          aaaaGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAgtgt                                       <  1:6269/141‑1 (MQ=255)                                                                agCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGAtgt                                 >  1:93745/1‑141 (MQ=255)                                                                    gagCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATgttgtt                             >  2:51809/1‑141 (MQ=255)                                                                     agCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTta                            <  1:128292/141‑1 (MQ=255)                                                                      gCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTtat                           <  1:116869/141‑1 (MQ=255)                                                                        aaaTATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTatat                         <  2:139028/141‑1 (MQ=255)                                                                                   aaaaaCAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCt              <  2:93745/141‑1 (MQ=255)                                                                                         aacCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAg        >  1:217485/1‑141 (MQ=255)                                                                                         aacCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAg        >  2:254878/1‑141 (MQ=255)                                                                                          acCTGAACTTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAgg       >  1:240644/1‑141 (MQ=255)                                                                                               aaaTTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATACTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTaaa  <  1:222975/141‑1 (MQ=255)                                                                                                                                      |                                                                                                      AATGGCAATGTTTGGTAATAAATACGAAGAGACACCTGATGTAATCGGGAAATCTGTAAAAGAAGCAGAGCAAATATTCAATAAAAACAACCTGAAATTGGGTAAAATTTCTAGAAGTTATAGTGATAAATATCCTGAAAATGAAATTATTAAGACAACTCCTAATACTGGTGAACGTGTTGAACGTGGTGACAGTGTTGATGTTGTTATATCAAAGGGCCCTGAAAAGGTTAAA  >  CP000730/1233199‑1233433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑