Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	849,263	C→A	A130E (GCA→GAA)	yvcJ →	ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	849,263	0	C	A	100.0%	127.7 / NA	38	A130E (GCA→GAA)	yvcJ	ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/20);  total (18/20)

CAAAAGTGAAAGTGACGTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGCAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTATGAAGATGAAGAGTTTGAAACTTTT  >  CP000730/849132‑849399                                                                                                                                    |                                                                                                                                         cAAAAGTGAAAGTGACGTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTTATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAatga                                                                                                                                 >  2:194777/1‑141 (MQ=255)  aaaaGTGAAAGTGACGTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAatgat                                                                                                                                >  2:445625/1‑141 (MQ=255)       tgaaagtgaCGTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCg                                                                                                                           <  1:336812/141‑1 (MQ=255)         aaagtgaCGTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCgag                                                                                                                         >  1:558238/1‑141 (MQ=255)           agtgaCGTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGagag                                                                                                                       <  2:454422/141‑1 (MQ=255)             tgaCGTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCa                                                                                                                     >  2:52264/1‑141 (MQ=255)                 gTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTg                                                                                                                 <  2:660010/141‑1 (MQ=255)                       atTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCaa                                                                                                           <  2:417621/141‑1 (MQ=255)                         tGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAAt                                                                                                         <  1:530202/141‑1 (MQ=255)                         tGATGTTATGTTTTTAGAAGCAAGTACAGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCATTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAAt                                                                                                         <  2:494651/141‑1 (MQ=255)                               tatgttTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAg                                                                                                   >  1:364171/1‑141 (MQ=255)                                atgttTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGt                                                                                                  >  2:516682/1‑141 (MQ=255)                                         gAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAAt                                                                                         >  1:481166/1‑141 (MQ=255)                                           aGCAAGGACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTGTGATGGAACAAGGTAAAAGATCGTTCATCAATGAAATTAATGATGGTCGAGAGCATTTGTCTCAAATTAGAAGTATAGCGAAttt                                                                                       <  2:3364/141‑1 (MQ=255)                                            gCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAAtttt                                                                                      <  2:27563/141‑1 (MQ=255)                                                   cTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAg                                                                               >  1:323438/1‑141 (MQ=255)                                                    tGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGa                                                                              <  2:58502/141‑1 (MQ=255)                                                           ttaattTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACa                                                                       <  1:462324/141‑1 (MQ=255)                                                               tttCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGt                                                                   >  2:651400/1‑141 (MQ=255)                                                               tttCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGt                                                                   >  2:310656/1‑141 (MQ=255)                                                                 tCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTa                                                                 <  2:214106/141‑1 (MQ=255)                                                                              aaaCGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAAt                                                    <  2:155320/141‑1 (MQ=255)                                                                                   cgtcgtGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAAGTAAGTATGAGCGTGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTaaaa                                               >  1:69801/1‑141 (MQ=255)                                                                                    gtcgtGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAg                                              >  2:9392/1‑141 (MQ=255)                                                                                       gtGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAAc                                           <  1:9392/141‑1 (MQ=255)                                                                                           acaTCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCAt                                       >  2:155980/1‑141 (MQ=255)                                                                                                  ttGATGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGa                                >  2:77026/1‑141 (MQ=255)                                                                                                     aTGGAACAAGGTAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATAc                             <  1:52264/141‑1 (MQ=255)                                                                                                             aGGTAAAAGAACGTTACCAAAAGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAAGTAAAAGAACGCATTCGTCGAAACTatgaaga                     >  2:703580/1‑141 (MQ=255)                                                                                                                tAAAAGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTatgaagatga                  >  2:567136/1‑141 (MQ=255)                                                                                                                 aaaaGATCGATAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTatgaagatgaa                 <  1:194777/141‑1 (MQ=255)                                                                                                                  aaaGATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTatgaagatgaag                >  2:596910/1‑141 (MQ=255)                                                                                                                     gATCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTATGAAGATGAAGAGt             <  1:58353/141‑1 (MQ=255)                                                                                                                      aTCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATATATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTATGAAGATGAAGAGtt            <  2:62205/141‑1 (MQ=255)                                                                                                                      aTCGTTAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTATGAAGATGAAGAGtt            <  2:481166/141‑1 (MQ=255)                                                                                                                          ttAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTATGAAGATGAAGAGTTTGaa        <  1:325419/141‑1 (MQ=255)                                                                                                                           tAATCAATGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTATGAAGATGAAGAGTTTGaaa       >  1:646984/1‑141 (MQ=255)                                                                                                                                aaTGAAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTATGAAGATGAAGAGTTTGAAACtttt  <  1:264989/141‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         CAAAAGTGAAAGTGACGTCATCATTGATGTTATGTTTTTAGAAGCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATGGAACAAGGTAAAAGATCGTTAATCAATGCAATTAATGATGAGCGAGAGCATTTGTCTCAAATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAAGAACGCATTCGTCGATACTATGAAGATGAAGAGTTTGAAACTTTT  >  CP000730/849132‑849399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑