Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,879 A→T 100% L11I (TTA→ATA)  mntB ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,8790AT100.0% 119.2 / NA 39L11I (TTA→ATA) mntBABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (21/18);  total (21/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAT  >  CP000730/707743‑708013
                                                                                                                                        |                                                                                                                                      
ccGGTATCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTataaa                                                                                                                                     <  2:490520/140‑1 (MQ=255)
      caaTTAAATTCCACAATTAAAATCTTGATAAGGGAAGATTTACCAGCACCATTCGGGCTTATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGAtt                                                                                                                               <  1:42186/138‑1 (MQ=255)
       cATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATCCCAATTATTTCCACGCGTCCGAGACGCGATAAGGAAAGGTTTTTAAGTACATGCTTATTACCTCTAAACCGCttt                                                                                                                              >  2:221205/1‑140 (MQ=255)
           aaaTTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAAt                                                                                                                          <  2:658553/140‑1 (MQ=255)
            aaTTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATc                                                                                                                         <  1:295093/140‑1 (MQ=255)
              ttCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCtt                                                                                                                       <  1:125957/140‑1 (MQ=255)
                   cAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGtt                                                                                                                  >  2:390385/1‑140 (MQ=255)
                        aaaGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTaa                                                                                                             <  2:153762/140‑1 (MQ=255)
                          tgtcgtGATAAGGGAAGATCTACACGCACCATTCGGTCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACa                                                                                                           <  2:550850/135‑1 (MQ=255)
                              ttGATATGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACg                                                                                                       <  1:280495/140‑1 (MQ=255)
                                 aTAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGttt                                                                                                    >  2:291912/1‑140 (MQ=255)
                                    aGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTtata                                                                                                 <  2:403076/140‑1 (MQ=255)
                                        aaGAGTTACCAGCACCGTGCGGTCCCAGGATACCAATTATTTCGCCGCGGACTGGTATCGATAAGGAAATGGTTTTAAGTACATTCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACTTTTATAcctc                                                                                             <  2:47978/140‑1 (MQ=255)
                                                ctagtaCCATTTGTGCCCATTATTCTAATTTTTTCGCCTCGTTCTGGTATTGATAATGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCttaattaa                                                                                     <  1:391760/135‑1 (MQ=255)
                                                         tttgggACCAGGATACAAATTATTTCTCCGCTTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAgg                                                                            <  1:208177/137‑1 (MQ=255)
                                                         ttCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAACCGTTTATACCTCCTAATTAAAAGTTTAgg                                                                            <  1:390385/140‑1 (MQ=255)
                                                          tCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAAACTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGc                                                                           >  1:679820/1‑140 (MQ=255)
                                                           cGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCt                                                                          >  1:291893/1‑140 (MQ=255)
                                                               cccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                      <  2:56445/140‑1 (MQ=255)
                                                                      tACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCtaattaa                                                               >  1:254398/1‑140 (MQ=255)
                                                                             tattTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                        >  2:525275/1‑140 (MQ=255)
                                                                               tttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTCTataat                                                      >  1:674840/1‑140 (MQ=255)
                                                                                 tCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTAtaataa                                                    >  1:206209/1‑140 (MQ=255)
                                                                                        gTATTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCGTTTGTTTCTAACCCCCGGTTATACCTCCCAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgaga                                             <  1:316249/140‑1 (MQ=255)
                                                                                        gTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTCCCTATAAACAGATTTAATTCTTTTGTTTCTAATAAACGTTTATACCACCTAATTAAAAATTTAGACTAACCTAATTAATTGTATAATAAACTgaga                                             >  1:194017/1‑140 (MQ=255)
                                                                                        gTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgaga                                             >  2:358819/1‑140 (MQ=255)
                                                                                             ggTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATAtt                                        >  1:187218/1‑140 (MQ=255)
                                                                                              gTATCGATAAGGAAATGTTGTTAAGTACATGCTTATTAACTATAAACAGATTTAACTCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAATTTACGCTAACCTAATTAATTGTATAATAAACTGAGaaagtat                                       >  2:282166/1‑135 (MQ=255)
                                                                                                aTCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTAt                                     <  2:412301/140‑1 (MQ=255)
                                                                                                   gATAAGGAAATGTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTCATTGTATAATAAAATGAGAATATTTATCAt                                  >  2:90519/1‑140 (MQ=255)
                                                                                                       aGGAAATGTTTTTAAGTAAATGTTTATTACCTATAAACAGATTTAATTCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGTATATTTATCATGTCa                              <  1:540750/140‑1 (MQ=255)
                                                                                                              gTTTTTAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACTTAATTAATTGTATAATAAACTGAGAATATTTATTATGTCAAGTAAAt                       >  1:83436/1‑140 (MQ=255)
                                                                                                                  ttAAGTACATGCTTATTCCCTATAACCAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAACAGTTTATGCTAACCTAATTAATTGTATAATAAACTGAGCATATTTATCATGTCAAGTAAATTCGt                   <  1:21227/140‑1 (MQ=255)
                                                                                                                  ttAAGTACATGCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGt                   <  2:340594/140‑1 (MQ=255)
                                                                                                                            gCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataatat         >  2:9801/1‑140 (MQ=255)
                                                                                                                            gCTTATTACCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataatat         >  1:628634/1‑140 (MQ=255)
                                                                                                                                attaCCAATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTAATATAATATAGAc     >  2:403266/1‑140 (MQ=255)
                                                                                                                                   aCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCTTGatataatgtaataaat  >  1:330044/1‑134 (MQ=255)
                                                                                                                                   aCCTATAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAat  >  1:618860/1‑140 (MQ=255)
                                                                                                                                   aCCTATAAACAGATTTAAATCTGTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAat  >  2:172961/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
CCGGTAGCATTAAATTCACCAATTAAAGACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAAT  >  CP000730/707743‑708013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑